BBa_K1456021
BBa_K1456021 Version 1 (Component)
Glutathione Peroxidase-1 (GPx-1) enzyme forward primer-2 with restriction site and kozak sequence

BBa_K1456022
BBa_K1456022 Version 1 (Component)
Glutathione Peroxidase(GPx1) enzyme reverse primer-2 with restriction site, two stop codon and 6xHis

BBa_M36998
BBa_M36998 Version 1 (Component)
Coenzyme B-12 Riboswitch for E. coli (nucleotides 1-240)

pBAD-AB-DT
BBa_K323135 Version 1 (Component)
VioA and VioB enzymes fused with zinc fingers under pBAD promoter

BBa_K1614025
BBa_K1614025 Version 1 (Component)
Ketamin-dependent self cleaving F8 - readout DNAzyme

BBa_J29021
BBa_J29021 Version 1 (Component)
This is 4 enzymes that is used to produce a β-carotene.

BBa_K1613012
BBa_K1613012 Version 1 (Component)
QsrR Binding Site inhibits the transcription and translation of Red Fluorescent Protein.

BBa_K1655001
BBa_K1655001 Version 1 (Component)
This GFP can be fused into any protein's aminoterminal end with the BioBrick enzyme assembly method.

pBAD-CDE-D
BBa_K323132 Version 1 (Component)
VioC, VioD and VioE enzymes fused with zinc fingers under pBAD promoter

BBa_M36979
BBa_M36979 Version 1 (Component)
Coenzyme B-12 Biosensor for E. coli (

BBa_M36980
BBa_M36980 Version 1 (Component)
Coenzyme B-12 Biosensor for E. coli (

BclI
BBa_M31987 Version 1 (Component)
BclI restriction enzyme site

PspOMI
BBa_M31984 Version 1 (Component)
PspOMI restriction enzyme site

Acc651
BBa_M31997 Version 1 (Component)
Acc65I restriction enzyme site

NheI
BBa_M31993 Version 1 (Component)
NheI restriction enzyme site

AvrII
BBa_M31994 Version 1 (Component)
AvrII restriction enzyme site

BBa_K1796115
BBa_K1796115 Version 1 (Component)
acetyl-Coenzyme A synthase Alpha subunit C-terminal domain

Propane1
BBa_K1655000 Version 1 (Component)
Propane 1 codes three of the ten enzymes necessary to produce propane in Escherichia coli.

BBa_K1961000
BBa_K1961000 Version 1 (Component)
CYP2A13 is a member of the Cytochrome P450 (CYP) enzymes family, which are critical for the metaboli

MfeI
BBa_B0047 Version 1 (Component)
MfeI restriction enzyme site (EcoRI compatible overhang)

AvrII
BBa_B0048 Version 1 (Component)
AvrII restriction enzyme site (XbaI, SpeI compatible overhang)

BBa_M36914
BBa_M36914 Version 1 (Component)
Codes for the benzylalcohol acetyltransferase enzyme

BBa_K1395000
BBa_K1395000 Version 1 (Component)
nrfA gene (Nitrite reductase enzyme) under constitutive promoter

BBa_K323164
BBa_K323164 Version 1 (Component)
VioA and VioB enzymes fused with zinc fingers under pBAD promoter in pSB4K5

BBa_K323163
BBa_K323163 Version 1 (Component)
VioC, VioD and VioE enzymes fused with zinc fingers under pBAD promoter in pSB4C5

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.