CR-1C3-HC
BBa_K590049 Version 1 (Component)
ADC-AAR-PSB1C3-High constitutive

BBa_J119405
BBa_J119405 Version 1 (Component)
(pSB1A2-BR) Deletion of the original -35 sequence

BBa_J119310
BBa_J119310 Version 1 (Component)
pSB1A2-BR-HS-G-IT-GGTT-RNA-I

BBa_K1058008
BBa_K1058008 Version 1 (Component)
Cr(VI) sensor with egfp(PchrB+chrB+egfp)

BBa_K1763003
BBa_K1763003 Version 1 (Component)
Major Spidroin Protein 2 (MaSp2) with sticky ends BC

BBa_K1114100
BBa_K1114100 Version 1 (Component)
MoClo format of a modified version of BBa_B0030 with BC fusion sites.

BBa_K1114102
BBa_K1114102 Version 1 (Component)
MoClo format of a modified version of BBa_B0032 with BC fusion sites

BBa_K1114101
BBa_K1114101 Version 1 (Component)
MoClo format of a modified version of BBa_B0031 with BC fusion sites.

BBa_K382033
BBa_K382033 Version 1 (Component)
EcR (Ecdysone Receptor)

BBa_K737000
BBa_K737000 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_K737001
BBa_K737001 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_J119061
BBa_J119061 Version 1 (Component)
pSB1A2-BR

BBa_J119309
BBa_J119309 Version 1 (Component)
pSB1A2-BR-HS-G-RNA-I

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.