BBa_K1963008
BBa_K1963008 Version 1 (Component)
Device for rhamnose-dependent expression of an OsmY fusion to E. coli Hfq..

BBa_K809716
BBa_K809716 Version 1 (Component)
GAL promoter + DNase + ADH1 terminator

BBa_J16104
BBa_J16104 Version 1 (Component)
HG-BDOM - Hunger generator with built in Banana-Dependant Output

BBa_K112111
BBa_K112111 Version 1 (Component)
Ligase without a start with a stop codon.

cin8
BBa_K105013 Version 1 (Component)
cin8 - cell cycle specific degradation tag in yeast

BBa_K2170211
BBa_K2170211 Version 1 (Component)
Secretory biotin ligase BirA (IRES_Kozak_IgKappa_BirA_StrepDEL) in RFC[10]

mdnC
BBa_K627003 Version 1 (Component)
ATP-grasp-type ligase from the mdn-cluster

BBa_K1952007
BBa_K1952007 Version 1 (Component)
Hydrazine Synthase subunit beta (Kust-2860) with strong RBS under lactose-inducible promoter

BBa_K1952008
BBa_K1952008 Version 1 (Component)
Hydrazine Synthase subunit alpa (Kust-2861) with strong RBS under Lac promoter control

LigA
BBa_K525740 Version 1 (Component)
DNA ligase from Escherichia coli (LigA) with His-tag

cI-Cro+
BBa_K1846005 Version 1 (Component)
cI-Cro bacteriophage lambda lytic cycle regulatory circuit

BBa_M31201
BBa_M31201 Version 1 (Component)
Yeast CLB1 promoter region, G2/M cell cycle specific

BBa_K142204
BBa_K142204 Version 1 (Component)
lac Promoter + RBS + T4 ligase + double terminator

TALEA-dife
BBa_K1189021 Version 1 (Component)
TALE-A ferritin di-subunit direct fusion w/ hisTag under lacI promoter, and linker

BBa_K1631013
BBa_K1631013 Version 1 (Component)
Pconst(Medium) - rbs - Colcin E3 - rbs - Colicin Immunity protein - dterm

BBa_K606047
BBa_K606047 Version 1 (Component)
PVeg SpoVG KinA

BBa_K1753000
BBa_K1753000 Version 1 (Component)
the omega subunit of E.coli RNA polymerase with T7 promoter, RBS and terminator(BBa_B0014)

BBa_K112112
BBa_K112112 Version 1 (Component)
Ligase with a start codon without a stop codon

BBa_K809717
BBa_K809717 Version 1 (Component)
GAL promoter + ZIM17 + DNase + ADH1 terminator

LigA
BBa_K525730 Version 1 (Component)
DNA ligase from Escherichia coli (LigA) with PT7 and RBS

BBa_K896599
BBa_K896599 Version 1 (Component)
Strophurus rankini voucher AMS R140490 NADH dehydrogenase subunit 4 (ND4) gene, partial cds; tRNA-Hi

BBa_K1129037
BBa_K1129037 Version 1 (Component)
4-coumarate-CoA ligase (4CL) under arabinose promoter

BBa_K809702
BBa_K809702 Version 1 (Component)
SP of ZIM17 + Bovine Pancreatic DNase I

BBa_K654059
BBa_K654059 Version 1 (Component)
Fatty Acyl-CoA Synthetase (Ligase) (fadD from E. coli) with 8-His Tag

BBa_K1976050
BBa_K1976050 Version 1 (Component)
Colicin E2 DNase domain with B0034 RBS

BBa_K258012
BBa_K258012 Version 1 (Component)
AI2-dependent KGF synthesis with RFP reporter and AHL production for Quaroum Sensin Death Mechanism

BBa_K142203
BBa_K142203 Version 1 (Component)
T4 ligase with RBS (BBa_B0030) and double terminator (BBa_B0015)

BBa_K1129042
BBa_K1129042 Version 1 (Component)
4-coumarate-CoA ligase (4CL) under pTET constitutive promoter

BBa_K1976054
BBa_K1976054 Version 1 (Component)
Colicin E2 DNase with B0034 and T7 promoter

BBa_K606052
BBa_K606052 Version 1 (Component)
PVeg SpoVG KinA double terminator

BBa_K174011
BBa_K174011 Version 1 (Component)
IPTG inducable kinA Bacillus sporulation trigger

BBa_I13563
BBa_I13563 Version 1 (Component)
BioBrick site flanked by RNase sites and hairpins for use in Screening Plasmids

BBa_K1976052
BBa_K1976052 Version 1 (Component)
Colicin E2 DNase domain and immunity Protein with Anderson Promoters

BBa_K809715
BBa_K809715 Version 1 (Component)
T7 promoter + yeast mt RBS(AI1)+ Bovine Pancreatic DNase I (mt)

BBa_K1976055
BBa_K1976055 Version 1 (Component)
T7 generator of the trypsin fragment of the Colicin E2 DNase

BBa_K1766008
BBa_K1766008 Version 1 (Component)
EnvZ_V5 osmoregulatory histidine kinase from <i>E.coli</i>.

BBa_K1766014
BBa_K1766014 Version 1 (Component)
EnvZ osmoregulatory histidine kinase from <i>E.coli.</i>

BBa_J92006
BBa_J92006 Version 1 (Component)
M. Ruminantium RNA polymerase Subunit A"

BBa_K549004
BBa_K549004 Version 1 (Component)
LacI promotor fused with the iron dependent regulator fur

BBa_K242999
BBa_K242999 Version 1 (Component)
Colicin E3 rRNAse domain

BBa_K1952012
BBa_K1952012 Version 1 (Component)
Hydrazine Synthase subunit alpha (Kust-2861) with LacZ reporter

BBa_K606046
BBa_K606046 Version 1 (Component)
KinA, sporulation gene for B. Subtilis

BBa_K809603
BBa_K809603 Version 1 (Component)
Bovine Pancreatic DNase I (in yeast mt codon table)

BBa_K1441013
BBa_K1441013 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag INSERT

BBa_K1441012
BBa_K1441012 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag In pGAPz alpha A

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.