BBa_K1059006
BBa_K1059006 Version 1 (Component)
DNA segment whose transcript can prevent mRNA degradation by RNaseE in two state .

BBa_K1179017
BBa_K1179017 Version 1 (Component)
A mutant DNA binding Cas9 with an N-terminal linker and BsaI site for ligation

BBa_K1179008
BBa_K1179008 Version 1 (Component)
A mutant DNA binding Cas9 with a C-terminal linker and BsaI site for ligation

iGEM 2019 Plates
iGEM_2019_Plates Version 1 (Collection)
384-well plates of dried DNA distributed by iGEM in Spring 2019

iGEM 2018 Plates
iGEM_2018_Plates Version 1 (Collection)
384-well plates of dried DNA distributed by iGEM in Spring 2018

BBa_K880001
BBa_K880001 Version 1 (Component)
Asymmetrically digestible reporter to assay the activity of DNA recombinases FimE K137007 and HbiF K

BBa_J22000
BBa_J22000 Version 1 (Component)
DnaA binding sequence - Px

BBa_K323151
BBa_K323151 Version 1 (Component)
DNA program 123456

BBa_K323152
BBa_K323152 Version 1 (Component)
DNA Program 12346

BBa_K801032
BBa_K801032 Version 1 (Component)
GAL4 DNA binding domain

BBa_K105101
BBa_K105101 Version 1 (Component)
Gal4 - DNA binding domain

BBa_K105100
BBa_K105100 Version 1 (Component)
LacI - DNA binding domain

BBa_K887011
BBa_K887011 Version 1 (Component)
DNA program : specific DNA sequence which can be recognized by zinc fingers

BBa_K1441013
BBa_K1441013 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag INSERT

BBa_K187254
BBa_K187254 Version 1 (Component)
folD, ORF, forward primer

BBa_K1441012
BBa_K1441012 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag In pGAPz alpha A

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.