BBa_K1824559BBa_K1824559 Version 1 (Component)RNA Thermometer FourU (Specially designed for J23119)
BBa_K624030BBa_K624030 Version 1 (Component)pYMB+[13-R]+[CHAMP-Y]
BBa_K209442BBa_K209442 Version 1 (Component)AarI B-C part, M3/M2 chimera
BBa_K1679017BBa_K1679017 Version 1 (Component)Promoter+RNA thermometer (FourU) +mRFP
BBa_K1368011BBa_K1368011 Version 1 (Component)Spinach Aptamer 13-2 RNA with Stabilizing tRNA Scaffold
BBa_K797016BBa_K797016 Version 1 (Component)Plasmid Backbone including M-13 primer region
BBa_K808026BBa_K808026 Version 1 (Component)pNB-Est 13: Esterase PET cleaving enzyme
BBa_K624017BBa_K624017 Version 1 (Component)pYMB essentials+[Y-13-R]+[CHAMP]
BBa_K624019BBa_K624019 Version 1 (Component)pYMB essentials+[13-R]+[Y-CHAMP]
BBa_K1875008BBa_K1875008 Version 1 (Component)Two repeated guide operators to make double operator 13
BBa_K1875009BBa_K1875009 Version 1 (Component)Three repeated guide operators to make triple operator 13
BBa_M302009BBa_M302009 Version 1 (Component)Region between HpaI site in gene II and BamHI site in gene III
BBa_K1875010BBa_K1875010 Version 1 (Component)Base 10 target mutation to make mutated guide operator 13
BBa_K624018BBa_K624018 Version 1 (Component)pYMB essentials+[YN-13-R]+[YC-CHAMP]
BBa_K624031BBa_K624031 Version 1 (Component)pYMB essentials+[YN-13-R]+[CHAMP-YC]
mdnA-myc-gBBa_K627006 Version 1 (Component)Fusion part of mdnA gene (from mdn-cluster) with myc-tag and gene III
BBa_K1676089BBa_K1676089 Version 1 (Component)Mutant 13 of LDH and wildtype LDP
BBa_M31517BBa_M31517 Version 1 (Component)Refactored M13KO7 genome from HpaI site in gene II through BamHI site in gene III
BBa_K1368010BBa_K1368010 Version 1 (Component)Spinach Aptamer 13-2 RNA driven by c1 promoter
BBa_M31114BBa_M31114 Version 1 (Component)M13, gene II from HpaI to the end [1-831bp]
BBa_K1875007BBa_K1875007 Version 1 (Component)A 20 bp target sequence and PAM to make guide operator 13
BBa_K137085BBa_K137085 Version 1 (Component)optimized (TA) repeat constitutive promoter with 13 bp between -10 and -35 elements
BBa_K2036028BBa_K2036028 Version 1 (Component)placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-ompA-iLDH-TT-pRM-RBS-beta-galactosidase
BBa_K2036027BBa_K2036027 Version 1 (Component)pRE-RBS-Cro-RBS-CII-TT-ptrp-RBS-CI-TT-pR-RBS-CIII-RBS-RFP-LAAssrAtag-TT-pRM-RBS-GFP-LVAssrAtag
M3 TTLBBa_K202003 Version 1 (Component)Hybrid promoter having multiple operator sites.
BBa_K2036024BBa_K2036024 Version 1 (Component)placm-pRE-RBS-Cro-RBS-CII-TT-patp2-RBS-CI-TT-pR-RBS-CIII-RBS-iLDH-TT-pRM-RBS-beta-galactosidase
BBa_K2050419BBa_K2050419 Version 1 (Component)RNA Polimerase III Promoter (H1 promoter)
BBa_M31786BBa_M31786 Version 1 (Component)1st half of Gene III- preBamHI cut
BBa_K2050422BBa_K2050422 Version 1 (Component)Mouse H1 promoter (RNA Polymerase III promoter)
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.