BBa_J100099BBa_J100099 Version 1 (Component)A promoter (CydAB) activated by the FNR enzyme
BBa_K748008BBa_K748008 Version 1 (Component)P2 promoter. P2 promoter can be activated by phosphorylated AgrA.
BBa_K748009BBa_K748009 Version 1 (Component)P3 promoter. P3 can be activated by phosphorylated AgrA.
BBa_K823041BBa_K823041 Version 1 (Component)PydfG promoter regulated by ecf41
BBa_K823042BBa_K823042 Version 1 (Component)PsspK promoter regulated by sigma G
BBa_K823048BBa_K823048 Version 1 (Component)PspoIVB promoter regulated by sigma G
BBa_K748004BBa_K748004 Version 1 (Component)P3+RBS+GFP+T. P3 can be activated by phosphorylated AgrA.
BBa_K748005BBa_K748005 Version 1 (Component)P2+RBS+GFP+T. P2 can be activated by phosphorylated AgrA.
BBa_K887011BBa_K887011 Version 1 (Component)DNA program : specific DNA sequence which can be recognized by zinc fingers
TBYBBa_K897720 Version 1 (Component)Antisense FtsZ protected by paired termini structure under T7 promoter
BBa_K750001BBa_K750001 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 1.0 strength)
BBa_K750002BBa_K750002 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 0.6 strength)
BBa_K750003BBa_K750003 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 0.3 strength)
BBa_K750004BBa_K750004 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 0.07 strength)
BBa_K750000BBa_K750000 Version 1 (Component)pBADGLT:unstable gfp expression device activated by arabinose
BBa_K750006BBa_K750006 Version 1 (Component)LuxR expression device activated by arabinose
BBa_K737023BBa_K737023 Version 1 (Component)Promoter that can be induced by phosphate.
BBa_K737026BBa_K737026 Version 1 (Component)Promoter that can be induced by phosphate and carbon starvation.
BBa_K737035BBa_K737035 Version 1 (Component)Spot42 generator controlled by IPTG
BBa_K737036BBa_K737036 Version 1 (Component)Spot42 generator controlled by aTc
BBa_K750005BBa_K750005 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 0.01 strength)
BBa_K737038BBa_K737038 Version 1 (Component)galK::GFP generator ligated to sRNA device controlled by IPTG
BBa_K737039BBa_K737039 Version 1 (Component)galK::GFP generator ligated to sRNA device controlled by aTc
P-alsTBBa_K818300 Version 1 (Component)Promoter alsT, repressed by TnrA during conversion of NH4 to Glutamine in B. subtilis.
BBa_K759001BBa_K759001 Version 1 (Component)Aggregation Module inducible by arabinose in E.coli.
BBa_K908015BBa_K908015 Version 1 (Component)Microcin B17, Gene A flanked by rbs and Attc sequence
BBa_K908016BBa_K908016 Version 1 (Component)Microcin B17, Gene B flanked by Attc sequence and rbs
BBa_K908017BBa_K908017 Version 1 (Component)Microcin B17, Gene C flanked by Attc sequence and rbs
BBa_K908018BBa_K908018 Version 1 (Component)Microcin B17, Gene F flanked by Attc sequence and rbs
BBa_K908019BBa_K908019 Version 1 (Component)Microcin C7, Gene E flanked by Attc sequence and rbs
BBa_K908020BBa_K908020 Version 1 (Component)Microcin C7, Gene C flanked by Attc sequence and rbs
BBa_K908021BBa_K908021 Version 1 (Component)Microcin C7, Gene F flanked by Attc sequence and rbs
BBa_K818100BBa_K818100 Version 1 (Component)Promoter sboA, upregulated by rotten meat volatiles in B. subtilis
BBa_K818200BBa_K818200 Version 1 (Component)Regulator fnr, upregulated by rotten meat volatiles in B. subtilis
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_K098986BBa_K098986 Version 1 (Component)lac QPI driven by high promoter
BBa_K098985BBa_K098985 Version 1 (Component)lac QPI driven by low promoter
BBa_K098981BBa_K098981 Version 1 (Component)tet QPI driven by high promoter
BBa_K098980BBa_K098980 Version 1 (Component)tet QPI driven by low promoter
cI 434BBa_R0052 Version 1 (Component)Promoter (434 cI regulated)
BBa_K098991BBa_K098991 Version 1 (Component)cI promoter with GFP reporter
BBa_S04138BBa_S04138 Version 1 (Component)cI QPI with GFP reporter system
BBa_K098988BBa_K098988 Version 1 (Component)temperature sensitive cI inducible system with GFP reporter and high promoter
BBa_K098987BBa_K098987 Version 1 (Component)temperature sensitive cI inducible system with GFP reporter and low promoter
BBa_K098982BBa_K098982 Version 1 (Component)lac inducible system with low promoter and GFP reporter
BBa_K086001BBa_K086001 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter
BBa_K086002BBa_K086002 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP
BBa_K086003BBa_K086003 Version 1 (Component)modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter
BBa_K082034BBa_K082034 Version 1 (Component)lacI regulated GFP generator