BBa_K908017
BBa_K908017 Version 1 (Component)
Microcin B17, Gene C flanked by Attc sequence and rbs

BBa_K908018
BBa_K908018 Version 1 (Component)
Microcin B17, Gene F flanked by Attc sequence and rbs

BBa_K908019
BBa_K908019 Version 1 (Component)
Microcin C7, Gene E flanked by Attc sequence and rbs

BBa_K908020
BBa_K908020 Version 1 (Component)
Microcin C7, Gene C flanked by Attc sequence and rbs

BBa_K908021
BBa_K908021 Version 1 (Component)
Microcin C7, Gene F flanked by Attc sequence and rbs

BBa_K750008
BBa_K750008 Version 1 (Component)
Quorum sensing system based on LuxI and LuxR to control the expression of parts behind

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K098986
BBa_K098986 Version 1 (Component)
lac QPI driven by high promoter

BBa_K098985
BBa_K098985 Version 1 (Component)
lac QPI driven by low promoter

BBa_K098981
BBa_K098981 Version 1 (Component)
tet QPI driven by high promoter

BBa_K098980
BBa_K098980 Version 1 (Component)
tet QPI driven by low promoter

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_S04138
BBa_S04138 Version 1 (Component)
cI QPI with GFP reporter system

BBa_K098988
BBa_K098988 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and high promoter

BBa_K098987
BBa_K098987 Version 1 (Component)
temperature sensitive cI inducible system with GFP reporter and low promoter

BBa_K098982
BBa_K098982 Version 1 (Component)
lac inducible system with low promoter and GFP reporter

rTT+pR
BBa_K093003 Version 1 (Component)
Convergent Promoter System: Forward Module: rTT+lambda pR

BBa_K086001
BBa_K086001 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

BBa_K086002
BBa_K086002 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP

BBa_K086003
BBa_K086003 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP reporter

rPlac+TT
BBa_K093004 Version 1 (Component)
Convergent Promoter System: Reverse Module: rPlac+TT

BBa_K116500
BBa_K116500 Version 1 (Component)
OmpF promoter that is activated or repressesed by OmpR according to osmolarity.

BBa_K142046
BBa_K142046 Version 1 (Component)
tet-controlled LacI generator

BBa_K142016
BBa_K142016 Version 1 (Component)
tet-controlled lacI IS mutant (R197A) generator

BBa_K142017
BBa_K142017 Version 1 (Component)
tet-controlled lacI IS mutant (R197F) generator

BBa_K142018
BBa_K142018 Version 1 (Component)
tet-controlled lacI IS mutant (T276A) generator

BBa_K142019
BBa_K142019 Version 1 (Component)
tet-controlled lacI IS mutant (T276F) generator

BBa_K142020
BBa_K142020 Version 1 (Component)
tet-controlled lacI IS mutant (R197A, T276A) generator

BBa_K142021
BBa_K142021 Version 1 (Component)
tet-controlled lacI IS mutant (R197A, T276F) generator

BBa_K082017
BBa_K082017 Version 1 (Component)
general recombine system

BBa_K142022
BBa_K142022 Version 1 (Component)
tet-controlled lacI IS mutant (R197F, T276A) generator

BBa_K142023
BBa_K142023 Version 1 (Component)
tet-controlled lacI IS mutant (R197F, T276F) generator

BBa_K142047
BBa_K142047 Version 1 (Component)
tet-controlled LacI generator with constitutive TetR expression cassette

BBa_K116001
BBa_K116001 Version 1 (Component)
nhaA promoter, that can be regulated by pH and nhaR protein.

BBa_J04430
BBa_J04430 Version 1 (Component)
GFP coding device switched on by IPTG

pLac+GFP
BBa_I763004 Version 1 (Component)
GFP coding device switched on by IPTG

BBa_K142048
BBa_K142048 Version 1 (Component)
promotorless lacI expression cassette followed by constitutively active TetR generator

BBa_K142041
BBa_K142041 Version 1 (Component)
arabinose controlled RMF generator

BBa_K142073
BBa_K142073 Version 1 (Component)
lac-controlled GFP generator with constitutive lacI expression cassette

BBa_K142074
BBa_K142074 Version 1 (Component)
lac-controlled GFP-LVA generator with constitutive lacI expression cassette

BBa_K142075
BBa_K142075 Version 1 (Component)
lac-controlled CFP generator with constitutive lacI expression cassette

BBa_K142076
BBa_K142076 Version 1 (Component)
lac-controlled RFP generator with constitutive lacI expression cassette

BBa_K079051
BBa_K079051 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac 1 operator

BBa_K079052
BBa_K079052 Version 1 (Component)
LacI repressor and GFP reporter proteins controlled by the J23118 promoter and Lac symmetric operato

BBa_K086004
BBa_K086004 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ24 followed by YFP promoter

BBa_K086005
BBa_K086005 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28 followed by YFP

BBa_K086006
BBa_K086006 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28 followed by YFP

BBa_K086007
BBa_K086007 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28 followed by YFP reporter

BBa_K086008
BBa_K086008 Version 1 (Component)
modified Lutz-Bujard LacO promoter,with alternative sigma factor σ28 followed by YFP