NAT (Nourseothricin Resistance/NrsR)
Nourseothricin_resistance Version 1 (Unknown)
Organism: Cryptococcus neoformans serotype A H99S strain

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnDatabase_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Cryptococcus neoformans Database in Synthetic Biology Open Language
CnD_collection Version 1 (Unknown)
This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.

Direct Sequence Pull
direct Version 1 (Activity)
Used to indicate that a sequence was pulled directly from a source with no alterations made

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Sequence Cut Out of Plasmid
cut_out_of_plasmid Version 1 (Activity)
Used to indicate that a sequence was cut out of the plasmid referenced as the source

Sequence Pull with Mutations
mutations Version 1 (Activity)
Used to indicate that a sequence had mutations added after pulling from the source and before submission

Reverse Orientation Sequence Pull
reverse_orientation Version 1 (Activity)
Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission

Unknown Alteration Sequence Pull
Unknown Version 1 (Activity)
Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown

Codon Optimised Sequence Pull
codon_optimisation Version 1 (Activity)
Used to indicate that a sequence was codon optimised after pulling from the source and before submission

HA
BBa_I712008 Version 1 (Component)
HA affinity tag

RGD-His-St
BBa_K133036 Version 1 (Component)
RGD-His affinity tag-Stop

His-Stop
BBa_K133133 Version 1 (Component)
His affinity tag-stop

Scar25 ATG
BBa_K1319106 Version 1 (Component)
RFC 25 Translation Start Scar Sequence

Cat
BBa_I14033 Version 1 (Component)
P(Cat)

Truncated Sequence Pull
Truncated Version 1 (Activity)
Used to indicate that a sequence was truncated after pulling from the source and before submission

BBa_J04031
BBa_J04031 Version 1 (Component)
GFP w/LVA tag

BBa_K1150016
BBa_K1150016 Version 1 (Component)
HA tag

NLS
BBa_J176008 Version 1 (Component)
NLS

His
BBa_K157011 Version 1 (Component)
His affinity tag; Freiburg standard

BBa_K105012
BBa_K105012 Version 1 (Component)
10 aa flexible protein domain linker

PEST
BBa_J176018 Version 1 (Component)
PEST

linker
BBa_K133132 Version 1 (Component)
8 aa protein domain linker

BBa_K157012
BBa_K157012 Version 1 (Component)
Strep affinity tag II; Freiburg standard

BBa_K844000
BBa_K844000 Version 1 (Component)
10x-Histidine (10x-His) Tag with double stop codon (TAATAA)

HAT
BBa_M0033 Version 1 (Component)
HAT affinity tag

c-Myc
BBa_I712007 Version 1 (Component)
c-Myc affinity tag

NLSmycSTOP
BBa_J176031 Version 1 (Component)
NLS myc stop

HA
BBa_K128001 Version 1 (Component)
HA affinity tag

G-His6(N)
BBa_K1362430 Version 1 (Component)
C-terminal His6 (N-Intein)

3x FLAG
BBa_J64005 Version 1 (Component)
3x FLAG affinity tag

BBa_K416003
BBa_K416003 Version 1 (Component)
Yeast Secretion Tag

RBS-GFP-Te
BBa_I763020 Version 1 (Component)
RBS-GFP (+LVA Tag)-Terminators

T7
BBa_M0028 Version 1 (Component)
T7 affinity tag

BBa_J70042
BBa_J70042 Version 1 (Component)
6his part for use with BioScaffold parts ATG-6his-GGATCC (glycine serine)

Tat PROM
BBa_K895000 Version 1 (Component)
Eco_Ptat2

IMPACT
BBa_M0026 Version 1 (Component)
IMPACT affinity tag

Biotin
BBa_M0024 Version 1 (Component)
Biotin affinity tag

FLAG
BBa_M0022 Version 1 (Component)
FLAG affinity tag

His
BBa_K128005 Version 1 (Component)
His affinity tag

polyA
BBa_J176009 Version 1 (Component)
polyA2

Strep-tag
BBa_M0031 Version 1 (Component)
Strep-tag II affinity tag

BBa_K1125001
BBa_K1125001 Version 1 (Component)
Tandem Affinity Purification (TAP) tag

c-Myc
BBa_M0032 Version 1 (Component)
c-Myc affinity tag

Poly-Arg
BBa_M0030 Version 1 (Component)
Poly-Arginine affinity tag

S-protein
BBa_M0029 Version 1 (Component)
S-protein affinity tag

BBa_K1583060
BBa_K1583060 Version 1 (Component)
Hydroxyapatite affinity tag

FLAG
BBa_K128000 Version 1 (Component)
FLAG affinity tag