ChZ N-fusi
BBa_K537007 Version 1 (Component)
RBS CheZ N-Fusion

SgsE|LigA
BBa_K525005 Version 1 (Component)
Fusion Protein of S-Layer SgsE and LigA

SgsE | Luc
BBa_K525311 Version 1 (Component)
Fusion Protein of S-Layer SgsE and Firefly-Luciferase

SbpA | Luc
BBa_K525411 Version 1 (Component)
Fusion Protein of S-Layer SbpA and Firefly-Luciferase

BisdAB
BBa_K525517 Version 1 (Component)
Fusion protein of BisdA and BisdB behind constitutive promoter

AAR-ADC
BBa_K590051 Version 1 (Component)
Acyl-ACP Reductase to Aldehyde Decarbonylase (AAR-ADC) Fusion

ADC-AAR
BBa_K590052 Version 1 (Component)
Aldehyde Decarbonylase to Acyl-ACP Reductase (ADC-AAR) Fusion

cph8
BBa_K592000 Version 1 (Component)
cph8 (Cph1/EnvZ fusion)

BBa_K638301
BBa_K638301 Version 1 (Component)
Arabinose inducible Reflectin A1-sfGFP fusion generator

CspB | RFP
BBa_K525234 Version 1 (Component)
Fusion Protein of mRFP, S-layer cspB from Corynebacterium halotolerans with TAT-sequence, PT7, RBS

mdnA-myc-g
BBa_K627006 Version 1 (Component)
Fusion part of mdnA gene (from mdn-cluster) with myc-tag and gene III

myc-gene I
BBa_K627007 Version 1 (Component)
Fusion of c-myc-tag and gene III

AraC_TEV-F
BBa_K627008 Version 1 (Component)
Fusion part of arabinose-inducible induction system and the TEV protease

AraC_TEV-F
BBa_K627010 Version 1 (Component)
Fusion of AraC induction system and TEV protease 3

AraC_14_3C
BBa_K627011 Version 1 (Component)
Fusion part of pBAD arabinose-inducible induction system and the HRV 14_3C protease

ssTorA_CS-
BBa_K627012 Version 1 (Component)
Fusion of TorA sig-seq, TEV protease cleavage site and b-lactamase

ssTorA_CS-
BBa_K627013 Version 1 (Component)
Fusion of ssTorA, cleavage site of 14_3C protease and b-lactamase

BBa_K615001
BBa_K615001 Version 1 (Component)
Zinc finger-omega subunit fusion protein

BBa_K654096
BBa_K654096 Version 1 (Component)
E. coli Reference Promoter (fusion of Anderson 0.51 and 1.00 promoters)

BBa_K737011
BBa_K737011 Version 1 (Component)
gvpC-GFP fusion protein with medium constitutive promoter

BBa_K737021
BBa_K737021 Version 1 (Component)
gvpC-20 coding gene with GFP mut 3.1 fusion gene

BBa_K737012
BBa_K737012 Version 1 (Component)
gvpC-GFP fusion protein UH with medium constitutive promoter

BBa_K737022
BBa_K737022 Version 1 (Component)
This part is gvpC-20 coding gene with GFP mut 3.1 fusion gene

BBa_K737013
BBa_K737013 Version 1 (Component)
gvpC-GFP fusion protein

BBa_K737014
BBa_K737014 Version 1 (Component)
gvpC-GFP fusion protein UH

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K125810
BBa_K125810 Version 1 (Component)
slr2016 signal sequence + GFP fusion for secretion of GFP

BBa_K105000
BBa_K105000 Version 1 (Component)
pSB1A2_fusion

BBa_J70032
BBa_J70032 Version 1 (Component)
A Composite BioScaffold Part (PpiI and PsrI) for Protein Fusions (see Design Page)

BBa_J70033
BBa_J70033 Version 1 (Component)
A BioMortar Part for Creating GSGS Protein Fusions (see Design page)

BBa_J70060
BBa_J70060 Version 1 (Component)
BBa_J70010 (PpiI) protein fusion test part

BBa_J70070
BBa_J70070 Version 1 (Component)
BBa_J70012 (PsrI) protein fusion test part

BBa_J70080
BBa_J70080 Version 1 (Component)
BBa_J70030 (PpiI) protein fusion test part

BBa_J70090
BBa_J70090 Version 1 (Component)
BBa_J70032 (PpiI/PsrI) protein fusion test part

BBa_J70061
BBa_J70061 Version 1 (Component)
BBa_J70009 (PpiI) protein fusion test part

BBa_E0051
BBa_E0051 Version 1 (Component)
lacZa.GFP fusion

BBa_J70121
BBa_J70121 Version 1 (Component)
R0040 regulated expression of a lacZa.GFP fusion protein

BBa_K257002
BBa_K257002 Version 1 (Component)
ClyA_Fusion_C-Term

tolRII
BBa_K257005 Version 1 (Component)
TolR domain II N-term fusion

BBa_K190027
BBa_K190027 Version 1 (Component)
MBP-ArsR fusion protein

BBa_K249004
BBa_K249004 Version 1 (Component)
N-terminal Arginine fusion vector

BBa_K249005
BBa_K249005 Version 1 (Component)
C-terminal Arginine Fusion Vector

BBa_K249006
BBa_K249006 Version 1 (Component)
Fusion EYFP with C-terminal Arginine Tag

BBa_K249007
BBa_K249007 Version 1 (Component)
CFP with C-terminal Arganine Fusion Tag

BBa_K249009
BBa_K249009 Version 1 (Component)
CFP with N-terminal Arganine Fusion Tag

clyA_Nter
BBa_K257007 Version 1 (Component)
ClyA_Fusion_N-Term

RFP_Nter
BBa_K257008 Version 1 (Component)
RFP_Fusion_NTerm

RFP_CTer
BBa_K257009 Version 1 (Component)
RFP_fusion_CTerm

BBa_K223061
BBa_K223061 Version 1 (Component)
hIL-6 + HlyAs Fusion protein (Freiburg Compatible)

TE3
BBa_K257022 Version 1 (Component)
TE3_fusion, domain of the colicin