BBa_K750004BBa_K750004 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 0.07 strength)
TBYBBa_K897720 Version 1 (Component)Antisense FtsZ protected by paired termini structure under T7 promoter
CBDcexBBa_K863101 Version 1 (Component)Cellulose binding Domain of Cellulomonas Fimi Exoglucanse (Freiburg-Standard)
BBa_K737058BBa_K737058 Version 1 (Component)Spot42 is a multitarget small RNA that mediates the discoordinate expression of the E.coli galactose
CBDclosBBa_K863111 Version 1 (Component)Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)
BBa_K737033BBa_K737033 Version 1 (Component)galK is the leader sequence of galactokinase (GalK)
BBa_K739003BBa_K739003 Version 1 (Component)promoter of raxRH + RBS + mRFP
BBa_K873003BBa_K873003 Version 1 (Component)conjugative transfer of RFG
BBa_K737048BBa_K737048 Version 1 (Component)The leading sequence involved in the nanC transcript which can act as the target of the sRNA, spot42
BBa_K737049BBa_K737049 Version 1 (Component)The leading sequence involved in the YtfJ transcript which can act as the target of the sRNA, spot42
BBa_K737050BBa_K737050 Version 1 (Component)The leading sequence involved in the SrlA transcript which can act as the target of the sRNA, spot42
BBa_K750005BBa_K750005 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 0.01 strength)
BBa_K750006BBa_K750006 Version 1 (Component)LuxR expression device activated by arabinose
BBa_K737023BBa_K737023 Version 1 (Component)Promoter that can be induced by phosphate.
BBa_K737026BBa_K737026 Version 1 (Component)Promoter that can be induced by phosphate and carbon starvation.
BBa_K737035BBa_K737035 Version 1 (Component)Spot42 generator controlled by IPTG
BBa_K737036BBa_K737036 Version 1 (Component)Spot42 generator controlled by aTc
BBa_K737038BBa_K737038 Version 1 (Component)galK::GFP generator ligated to sRNA device controlled by IPTG
BBa_K737039BBa_K737039 Version 1 (Component)galK::GFP generator ligated to sRNA device controlled by aTc
BBa_K777101BBa_K777101 Version 1 (Component)flhDC operon under the control of constitutive promoter J23100
BBa_K750008BBa_K750008 Version 1 (Component)Quorum sensing system based on LuxI and LuxR to control the expression of parts behind
BBa_K777102BBa_K777102 Version 1 (Component)flhDC operon under the control of constitutive promoter J23104
BBa_K777103BBa_K777103 Version 1 (Component)flhDC operon under the control of constitutive promoter J23105
BBa_K777104BBa_K777104 Version 1 (Component)flhDC operon under the control of constitutive promoter J23106
BBa_K777105BBa_K777105 Version 1 (Component)flhDC operon under the control of constitutive promoter J23109
BBa_K777106BBa_K777106 Version 1 (Component)flhDC operon under the control of constitutive promoter J23112
BBa_K777107BBa_K777107 Version 1 (Component)flhDC operon under the control of constitutive promoter J23113
BBa_K777108BBa_K777108 Version 1 (Component)flhDC operon under the control of constitutive promoter J23114
BBa_K769002BBa_K769002 Version 1 (Component)Kinase domain of EnvZ
CBDcex(T7)BBa_K863102 Version 1 (Component)Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)
BBa_K818100BBa_K818100 Version 1 (Component)Promoter sboA, upregulated by rotten meat volatiles in B. subtilis
BBa_K818200BBa_K818200 Version 1 (Component)Regulator fnr, upregulated by rotten meat volatiles in B. subtilis
P-alsTBBa_K818300 Version 1 (Component)Promoter alsT, repressed by TnrA during conversion of NH4 to Glutamine in B. subtilis.
BBa_K759001BBa_K759001 Version 1 (Component)Aggregation Module inducible by arabinose in E.coli.
BBa_K908015BBa_K908015 Version 1 (Component)Microcin B17, Gene A flanked by rbs and Attc sequence
BBa_K908016BBa_K908016 Version 1 (Component)Microcin B17, Gene B flanked by Attc sequence and rbs
BBa_K908017BBa_K908017 Version 1 (Component)Microcin B17, Gene C flanked by Attc sequence and rbs
BBa_K908018BBa_K908018 Version 1 (Component)Microcin B17, Gene F flanked by Attc sequence and rbs
BBa_K908019BBa_K908019 Version 1 (Component)Microcin C7, Gene E flanked by Attc sequence and rbs
BBa_K908020BBa_K908020 Version 1 (Component)Microcin C7, Gene C flanked by Attc sequence and rbs
BBa_K908021BBa_K908021 Version 1 (Component)Microcin C7, Gene F flanked by Attc sequence and rbs
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
BBa_K098984BBa_K098984 Version 1 (Component)lac inducible mtrB (high expression of QPI)
BBa_K098983BBa_K098983 Version 1 (Component)lac inducible mtrB (low expression of QPI)
BBa_K157002BBa_K157002 Version 1 (Component)Transmembrane region of the EGF-Receptor (ErbB-1)
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_K098986BBa_K098986 Version 1 (Component)lac QPI driven by high promoter
BBa_K098985BBa_K098985 Version 1 (Component)lac QPI driven by low promoter
BBa_K098981BBa_K098981 Version 1 (Component)tet QPI driven by high promoter