BBa_J70560
BBa_J70560 Version 1 (Component)
GFP, with N-terminal 6 his

BBa_K1845001
BBa_K1845001 Version 1 (Component)
Miraculin (Yeast codon optimised + His-tag)

BBa_K1732010
BBa_K1732010 Version 1 (Component)
pSB1C3-J23100-B0034-E0040-His-B0015

BBa_K2150010
BBa_K2150010 Version 1 (Component)
This part consists of a gene encoding toxin 134 with a LacI gene, a Ptac promoter included

BBa_K792000
BBa_K792000 Version 1 (Component)
Yeast exportable His-rich peptide w/enhanced import (nonStd)

BBa_K1441013
BBa_K1441013 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag INSERT

BBa_S03737
BBa_S03737 Version 1 (Component)
pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB1A2)

BBa_K1974011
BBa_K1974011 Version 1 (Component)
T7 Promoter+RBS+Hv1a+linker+6X His-Tag

BBa_K1974013
BBa_K1974013 Version 1 (Component)
T7 Promoter+RBS+OAIP+linker+6X His-Tag

BBa_K737001
BBa_K737001 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_K1989037
BBa_K1989037 Version 1 (Component)
Triplespytag with mRFP, His-tag , FlAsH-tag and signal peptide PhoA

BBa_K1974022
BBa_K1974022 Version 1 (Component)
T7Promoter+RBS+Sf1a+linker+snowdrop-lectin+linker+6X His-Tag

BBa_K1974021
BBa_K1974021 Version 1 (Component)
T7Promoter+RBS+Hv1a+linker+snowdrop-lectin+linker+6X His-Tag

BBa_K1974023
BBa_K1974023 Version 1 (Component)
T7Promoter+RBS+OAIP+linker+snowdrop-lectin+linker+6X His-Tag

BBa_J70084
BBa_J70084 Version 1 (Component)
Adds 6 his suffix, using BioScaffold part J70030 (PpiI) in pSB1AT3

BBa_J70604
BBa_J70604 Version 1 (Component)
J70589 cut with BsaXI and with rbs, 6his inserts (J70559-f1i,f2i,r2i,r2i) added

BBa_K1441012
BBa_K1441012 Version 1 (Component)
DNA ligase from Escherichia coli with His-tag In pGAPz alpha A

BBa_K1974033
BBa_K1974033 Version 1 (Component)
T7 Promoter+RBS+Hv1a+GS linker+snowdrop-lectin+linker+6X His-Tag

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.