BBa_K1431301
BBa_K1431301 Version 1 (Component)
TRE-3G promoter+SV40 PolyA, an ideal controller of mammalian gene expression with Tet-On 3G protein

BBa_J06612
BBa_J06612 Version 1 (Component)
Construction intermediate: LacI ts (mut 265) QPI with Lambda cI regulated promoter (R0051.J06801)

BBa_J06611
BBa_J06611 Version 1 (Component)
Construction intermediate: LacI ts (mut 241) QPI with Lambda cI regulated promoter (R0051.J06800)

BBa_K1638030
BBa_K1638030 Version 1 (Component)
Leucine zipper fused to T25 domain of <i>cyaA</i> with cAMP-induced RFP generator

BBa_K1638031
BBa_K1638031 Version 1 (Component)
Leucine zipper fused to T18 domain of <i>cyaA</i> with cAMP-induced RFP generator

BBa_K1159309
BBa_K1159309 Version 1 (Component)
19 AA Linker with N-terminal <i>Strep</i>-tag II and C-terminal TEV cleavage site in RFC[25]

BBa_K1985014
BBa_K1985014 Version 1 (Component)
Sequence coding for Sup35(residues 1-61) and Cytochrome b562 with an arabinose inducible promoter

BBa_K175050
BBa_K175050 Version 1 (Component)
aTc inducible GFP-LVA generator with integrated I-SceI RS + I-SceI RS + TetR generator

BBa_K2082231
BBa_K2082231 Version 1 (Component)
RFP under the control of an optimized lacZ promoter combined with the protein SH2:cI434

BBa_J37017
BBa_J37017 Version 1 (Component)
AiiA Expression Assay (can be used in conjunction with AHL assay to detect activity)

BBa_J120030
BBa_J120030 Version 1 (Component)
Composite part with Yeast promoter,CH1 domain of human Ab, ADAM17 cleavage sequence & Phoratoxin.

BBa_K606012
BBa_K606012 Version 1 (Component)
RFP mCherry with double terminator

BBa_K1159120
BBa_K1159120 Version 1 (Component)
Red light triggered TEV Protease with FRET Reporter for plants translation unit (PhyB/PIF6 version)

GFPLVA
BBa_K411207 Version 1 (Component)
Green Fluorescent Protein with LVA tag (GFPLVA)

BBa_K1994022
BBa_K1994022 Version 1 (Component)
Multiple dCas9 binding sites with weak omega promoter upstream of GFP + dCas9 expression cassette

BBa_K2055015
BBa_K2055015 Version 1 (Component)
Promoter + Inverter with Ferric Uptake Regulator & LacI. Activates gene expression in presence of Fe

BBa_K1362106
BBa_K1362106 Version 1 (Component)
RBS + NpuDnaE N-intein RFC[105] assembly construct with additional C-teminal insertion site

BBa_K1362107
BBa_K1362107 Version 1 (Component)
RBS + NpuDnaE C-intein RFC[105] assembly construct with additional N-teminal insertion site

BBa_K1039022
BBa_K1039022 Version 1 (Component)
PhiC31 integrase and PhiC31 RDF under lock and key control with lacI Expression Cassette

BBa_K1039021
BBa_K1039021 Version 1 (Component)
Bxb1 integrase and Recombination Directionality Factor (RDF) Under ???Lock and Key??? Control with L

BBa_K1159119
BBa_K1159119 Version 1 (Component)
Red light triggered TEV Protease with FRET Reporter for plants translation unit (PhyB/PIF3 version)

BBa_J06550
BBa_J06550 Version 1 (Component)
Construction intermediate: lambda cI with RBS (B0034.C0051)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1362061
BBa_K1362061 Version 1 (Component)
N-terminal chitin binding domain with triglycine linker and His-tag as RFC[105] insert

BBa_K901010
BBa_K901010 Version 1 (Component)
AatA with dT

BBa_K1361004
BBa_K1361004 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag

BBa_K1994060
BBa_K1994060 Version 1 (Component)
Multiple dCas9 binding sites with weak sigma 54 promoter upstream of GFP + dCas9 expression cassette

BBa_K1088009
BBa_K1088009 Version 1 (Component)
B. subtilis dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG ind

BBa_K1088010
BBa_K1088010 Version 1 (Component)
E. coli dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG inducib

BBa_K258012
BBa_K258012 Version 1 (Component)
AI2-dependent KGF synthesis with RFP reporter and AHL production for Quaroum Sensin Death Mechanism

BBa_K1362166
BBa_K1362166 Version 1 (Component)
RBS + Gp41-1 N-intein RFC[105] assembly construct with additional C-teminal insertion site

BBa_K176249
BBa_K176249 Version 1 (Component)
Low input score automatic selector w/o lethal device(with reporter): PoPS->mRFP->CI-LVA+pCI->K176240

BBa_K1362167
BBa_K1362167 Version 1 (Component)
RBS + Gp41-1 C-intein RFC[105] assembly construct with additional N-teminal insertion site

BBa_I742118
BBa_I742118 Version 1 (Component)
CrtZ with native ribosome binding site

luxR-ndh
BBa_K1036000 Version 1 (Component)
lux pL controlled luxR with lux pR controlled ndh (LVA-tag) coding for NADH dehydrogenase II

BBa_K175035
BBa_K175035 Version 1 (Component)
Constitutive expression of GFP with medium RBS lock and inducible production of key for the lock

BBa_K175034
BBa_K175034 Version 1 (Component)
Constitutive expression of GFP with weak RBS lock and inducible production of key for the lock

BBa_K737000
BBa_K737000 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5&#945;,using PCR with the primers we desig

BBa_K238028
BBa_K238028 Version 1 (Component)
ech with RBS

RFPLVA
BBa_K411208 Version 1 (Component)
Red Fluorescent Protein with LVA tag (RFPLVA)

BBa_M11085
BBa_M11085 Version 1 (Component)
E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o

rbs+crtY
BBa_I742155 Version 1 (Component)
crtY (lycopene cyclase) with native rbs

BBa_K077020
BBa_K077020 Version 1 (Component)
ptet with LasR

BBa_K1773010
BBa_K1773010 Version 1 (Component)
pLuxcI right promoter with medium RBS

BBa_K1412999
BBa_K1412999 Version 1 (Component)
GFP generator with J23115

BBa_K1412716
BBa_K1412716 Version 1 (Component)
GFP generator with J23101

BBa_I742106
BBa_I742106 Version 1 (Component)
Sam8 gene with ribosome binding site

BBa_I742105
BBa_I742105 Version 1 (Component)
Sam5 gene with ribosome binding site

BBa_K648031
BBa_K648031 Version 1 (Component)
RBS with cI repressor

BBa_K1834846
BBa_K1834846 Version 1 (Component)
Xylene Sensor! With AmilCP!