BBa_J72161BBa_J72161 Version 1 (Component)ColE2 origin of replication
BBa_J119123BBa_J119123 Version 1 (Component)Promoter-tryptophan, Ptrp, repressible promoter of the tryptophan operon
BBa_K812013BBa_K812013 Version 1 (Component)GFP-AID OsTirI polysistronic system for auxin detection in tadpole
BBa_J72210BBa_J72210 Version 1 (Component)Kanamycin resistant BAC with R6K origin, pGlpT 5' of cloning site
BBa_K737000BBa_K737000 Version 1 (Component)We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig
BBa_K737001BBa_K737001 Version 1 (Component)We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig
BBa_K758003BBa_K758003 Version 1 (Component)UAS, this part consists of five UAS sequences and hsp70 TATA.
UAS-LacZBBa_K758005 Version 1 (Component)UAS LacZ, this part expresses LacZ in the presence of GAL4
UAS-EGFPBBa_K758006 Version 1 (Component)UAS EGFP, this part expresses EGFP in the presence of GAL4 protein.
BBa_K812012BBa_K812012 Version 1 (Component)OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin
BBa_K750001BBa_K750001 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 1.0 strength)
BBa_K750002BBa_K750002 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 0.6 strength)
BBa_K750003BBa_K750003 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 0.3 strength)
BBa_K750004BBa_K750004 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 0.07 strength)
BBa_K737026BBa_K737026 Version 1 (Component)Promoter that can be induced by phosphate and carbon starvation.
BBa_K750005BBa_K750005 Version 1 (Component)LuxI expression device activated by arabinose(Regulated by RBS of 0.01 strength)
CBDcexBBa_K863101 Version 1 (Component)Cellulose binding Domain of Cellulomonas Fimi Exoglucanse (Freiburg-Standard)
BBa_K737058BBa_K737058 Version 1 (Component)Spot42 is a multitarget small RNA that mediates the discoordinate expression of the E.coli galactose
CBDclosBBa_K863111 Version 1 (Component)Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)
BBa_K737033BBa_K737033 Version 1 (Component)galK is the leader sequence of galactokinase (GalK)
BBa_K739003BBa_K739003 Version 1 (Component)promoter of raxRH + RBS + mRFP
BBa_K873003BBa_K873003 Version 1 (Component)conjugative transfer of RFG
BBa_K737048BBa_K737048 Version 1 (Component)The leading sequence involved in the nanC transcript which can act as the target of the sRNA, spot42
BBa_K737049BBa_K737049 Version 1 (Component)The leading sequence involved in the YtfJ transcript which can act as the target of the sRNA, spot42
BBa_K737050BBa_K737050 Version 1 (Component)The leading sequence involved in the SrlA transcript which can act as the target of the sRNA, spot42
BBa_K777101BBa_K777101 Version 1 (Component)flhDC operon under the control of constitutive promoter J23100
BBa_K750008BBa_K750008 Version 1 (Component)Quorum sensing system based on LuxI and LuxR to control the expression of parts behind
P-alsTBBa_K818300 Version 1 (Component)Promoter alsT, repressed by TnrA during conversion of NH4 to Glutamine in B. subtilis.
BBa_K777102BBa_K777102 Version 1 (Component)flhDC operon under the control of constitutive promoter J23104
BBa_K777103BBa_K777103 Version 1 (Component)flhDC operon under the control of constitutive promoter J23105
BBa_K777104BBa_K777104 Version 1 (Component)flhDC operon under the control of constitutive promoter J23106
BBa_K777105BBa_K777105 Version 1 (Component)flhDC operon under the control of constitutive promoter J23109
BBa_K777106BBa_K777106 Version 1 (Component)flhDC operon under the control of constitutive promoter J23112
BBa_K777107BBa_K777107 Version 1 (Component)flhDC operon under the control of constitutive promoter J23113
BBa_K777108BBa_K777108 Version 1 (Component)flhDC operon under the control of constitutive promoter J23114
BBa_K769002BBa_K769002 Version 1 (Component)Kinase domain of EnvZ
CBDcex(T7)BBa_K863102 Version 1 (Component)Cellulose binding Domain of C. Fimi Exoglucanase with T7, RBS, GS-Linker (Freiburg-Standard)
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_K098984BBa_K098984 Version 1 (Component)lac inducible mtrB (high expression of QPI)
BBa_K157002BBa_K157002 Version 1 (Component)Transmembrane region of the EGF-Receptor (ErbB-1)
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
BBa_K098983BBa_K098983 Version 1 (Component)lac inducible mtrB (low expression of QPI)
BBa_K125810BBa_K125810 Version 1 (Component)slr2016 signal sequence + GFP fusion for secretion of GFP
OriTRBBa_J01003 Version 1 (Component)OriT-R (Origin of transfer for the R-plasmid nic region)
BBa_K156009BBa_K156009 Version 1 (Component)OFP (orange fluorescent protein)
BBa_K116510BBa_K116510 Version 1 (Component)GFP under control of pOmpf promoter(K116500+E0240)
BBa_K142024BBa_K142024 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (R197A) and TetR expression cassette
BBa_K142025BBa_K142025 Version 1 (Component)IPTG-on tetracycline-off pulse generator with LacI mutant (R197F) and TetR expression cassette