GFPGFP Version 1 (Unknown)Organism: Cryptococcus neoformans serotype A H99S
iGEM Feature Librariesigem_feature_libraries_collection Version 1 (Unknown)A collection of iGEM feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
Free Genes Feature Librariesfree_genes_feature_libraries_collection Version 1 (Unknown)A collection of Free Genes feature libraries for use with Synthetic Biology Curation Tools (SYNBICT).
HYGHygromicin_Resistance Version 1 (Unknown)Organism: Cryptococcus neoformans serotype A strain H99O
mCherrymCherry Version 1 (Unknown)Organism: Cryptococcus neoformans serotype A H99S strain
amdSamdS Version 1 (Unknown)Organism: Cryptococcus neoformans serotype A strain H99O
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnDatabase_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
Cryptococcus neoformans Database in Synthetic Biology Open LanguageCnD_collection Version 1 (Unknown)This is a collection of Biological Genetic Parts of Cryptococcus neoformans containing 23 different promoters, coding sequences and terminators, which were previously used and whose efficacy has already been proven in this organism. This work was made in order to facilitate the build and assembly of genetic circuits and plasmids for the study of this pathogen.
APP1GenBank_CP047908_Region_1244167_1244926 Version 1 (Unknown)Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)
ACT1GenBank_CP003820_Region_1242235_1243067 Version 1 (Unknown)Organism: Cryptococcus neoformans var. grubii H99 (Cryptococcus neoformans serotype A)
ACS Synbio GenerationACS_Synbio_Generation Version 1 (Activity)Sequences pulled from the supplementals of the ACS Synthetic Biology journal as part of the SBKS project
SBOLDesigner CAD ToolSBOLDesigner Version 3.1 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.
Direct Sequence Pulldirect Version 1 (Activity)Used to indicate that a sequence was pulled directly from a source with no alterations made
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
Sequence Cut Out of Plasmidcut_out_of_plasmid Version 1 (Activity)Used to indicate that a sequence was cut out of the plasmid referenced as the source
Unknown Alteration Sequence PullUnknown Version 1 (Activity)Used to indicate that a sequence alteration was made between the source and the submission but the nature of the alteration is unknown
Codon Optimised Sequence Pullcodon_optimisation Version 1 (Activity)Used to indicate that a sequence was codon optimised after pulling from the source and before submission
Sequence Pull with Mutationsmutations Version 1 (Activity)Used to indicate that a sequence had mutations added after pulling from the source and before submission
GFPBBa_E0040 Version 1 (Component)green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
Reverse Orientation Sequence Pullreverse_orientation Version 1 (Activity)Used to indicate that a sequence's orientation was reversed (e.g. 3' to 5' becomes 5' to 3') after pulling from the source and before submission
SBOLDesigner CAD ToolSBOLDesigner Version 2.2 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2.0 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.
ATGATG Version 1 (Component) BBa_B0032BBa_B0032 Version 1 (Component)RBS.3 (medium) -- derivative of BBa_0030
eyfpBBa_E0030 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
Scar25 ATGBBa_K1319106 Version 1 (Component)RFC 25 Translation Start Scar Sequence
mRFP1BBa_E1010 Version 1 (Component)**highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
YFPBBa_E0032 Version 1 (Component)enhanced yellow fluorescent protein derived from A. victoria GFP
BBa_K1333005BBa_K1333005 Version 1 (Component)M13 bacteriophage g8p with a FLAG tag on C-terminal
BBa_K1333002BBa_K1333002 Version 1 (Component)M13 bacteriophage g3p with start codon of "ATG" and a FLAG tag on C-terminal
ecfpBBa_E0020 Version 1 (Component)engineered cyan fluorescent protein derived from A. victoria GFP
BBa_B0031BBa_B0031 Version 1 (Component)RBS.2 (weak) -- derivative of BBa_0030
GFP reportBBa_E0240 Version 1 (Component)GFP generator
BBa_I712074BBa_I712074 Version 1 (Component)T7 promoter (strong promoter from T7 bacteriophage)
RFC105 ABBa_K1362414 Version 1 (Component)N-terminal start overhang (T)(A)-(G)ATG=RBS+Start RFC[105] A
BBa_K844000BBa_K844000 Version 1 (Component)10x-Histidine (10x-His) Tag with double stop codon (TAATAA)
BBa_J04031BBa_J04031 Version 1 (Component)GFP w/LVA tag
ECFPBBa_E0022 Version 1 (Component)enhanced cyan fluorescent protein derived from A. victoria GFP
BBa_K809601BBa_K809601 Version 1 (Component)GAL promoter (kozak) without start codon ATG
BBa_K1333004BBa_K1333004 Version 1 (Component)M13 bacteriophage g5p with a FLAG tag on C-terminal
BBa_J04500BBa_J04500 Version 1 (Component)IPTG inducible promoter with RBS
His-StopBBa_K133133 Version 1 (Component)His affinity tag-stop
BBa_K249006BBa_K249006 Version 1 (Component)Fusion EYFP with C-terminal Arginine Tag
GFP generaBBa_E0840 Version 1 (Component)GFP generator
BBa_K209402BBa_K209402 Version 1 (Component)AarI A-B part, signal sequence-FLAG-YFP [SSFYFP]