BBa_M39401
BBa_M39401 Version 1 (Component)
Actin

ygiV
BBa_K200002 Version 1 (Component)
Colanic acid global regulator -> ygiV (B3023)

BBa_I725023
BBa_I725023 Version 1 (Component)
RBS + Butyryl coa Dehydrogenase

BBa_I725022
BBa_I725022 Version 1 (Component)
RBS + Enoyl-coa hydratase

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K612005
BBa_K612005 Version 1 (Component)
Actin Promoter

BBa_I737001
BBa_I737001 Version 1 (Component)
protein generator for XXXX for making YYYY

GRE3
BBa_K1602004 Version 1 (Component)
Aldose reductase (GRE3) from Saccharomyces cerevisiae S288c

BBa_K929302
BBa_K929302 Version 1 (Component)
AID in Potsdam Standard

BBa_K316003
BBa_K316003 Version 1 (Component)
XylE - catechol 2,3-dioxygenase from P.putida with terminator

BBa_K883404
BBa_K883404 Version 1 (Component)
PLRV Coat Protein gene - Isolated from Marfona potato

BBa_K883402
BBa_K883402 Version 1 (Component)
PLRV Coat Protein gene - Isolated from Bildstar potato

BBa_K2007002
BBa_K2007002 Version 1 (Component)
Drosophila melanogaster Juvenile Hormone Acid O-methyltransferase (DmJHAMT)

BBa_K499595
BBa_K499595 Version 1 (Component)
luxC gene encoding acyl coA reductase

BBa_I725003
BBa_I725003 Version 1 (Component)
B-hydroxybutyryl coA dehydrogenase (2)

BBa_I725002
BBa_I725002 Version 1 (Component)
B-hydroxybutyryl coA dehydrogenase (1)

BBa_K499999
BBa_K499999 Version 1 (Component)
luxC gene encoding acyl coA reductase

FUS1 pr
BBa_K1154001 Version 1 (Component)
Saccharomyces cerevisiae FUS1 promoter (induced by mating pheromone)

ClpX
BBa_K365004 Version 1 (Component)
ClpX from E.coli (aa 61-425)

BBa_K1181000
BBa_K1181000 Version 1 (Component)
Kaurenoic Acid Hydroxylase

BBa_K638001
BBa_K638001 Version 1 (Component)
Reflectin A1 from Loligo

BBa_K1529972
BBa_K1529972 Version 1 (Component)
oxalic acid

p_qrr4
BBa_K131017 Version 1 (Component)
p_qrr4 from <I>Vibrio harveyi</I>

BBa_M39012
BBa_M39012 Version 1 (Component)
Sialic Acid Receptor

BBa_K1403009
BBa_K1403009 Version 1 (Component)
Benzoic acid/salicylic acid carboxyl methyltransferase 1 (BSMT1) expression cassette

BBa_K1472608
BBa_K1472608 Version 1 (Component)
Oleic acid (18:0) -> alpha-Linolenic acid (18:3-9,12,15)

BBa_M1679
BBa_M1679 Version 1 (Component)
Fatty acid synthetase promoter

BBa_I746365
BBa_I746365 Version 1 (Component)
PLL promoter from P4 phage

SAMT
BBa_J45001 Version 1 (Component)
SAM:salicylic acid carboxyl methyltransferase; converts salicylic acid to methyl salicylate (winter

BBa_K137006
BBa_K137006 Version 1 (Component)
pabB (from pABA synthesis)

BBa_K1211001
BBa_K1211001 Version 1 (Component)
Clostridum propionicum propionate CoA transferase

BBa_K203106
BBa_K203106 Version 1 (Component)
HMG-CoA synthase promoter (sterol regulated)

BBa_I757000
BBa_I757000 Version 1 (Component)
Feruloy CoA hydratase 4 vanillin biosynthesis

BBa_K223043
BBa_K223043 Version 1 (Component)
RALDH II gene: Converts retinal into retinoic acid

BBa_B0023
BBa_B0023 Version 1 (Component)
TE from coliphage T7, reversed

BBa_I11031
BBa_I11031 Version 1 (Component)
excisionase from E. coli phage P22 (removes prophage from host genome)

BBa_K1216006
BBa_K1216006 Version 1 (Component)
Acetyl esterase (aes) from Escherichia Coli with TEV and poly-HIS tags

BBa_I744002
BBa_I744002 Version 1 (Component)
feruloyl-CoA hydratase for biosynthesis of vanillin

BBa_K177026
BBa_K177026 Version 1 (Component)
listeriolysin O 399G->C - locus from Listeria monocytogenes

BBa_I725004
BBa_I725004 Version 1 (Component)
Enoyl-coA hydratase (crotonase) (16)

BBa_K797000
BBa_K797000 Version 1 (Component)
tatABCD from E.coli

BBa_K883403
BBa_K883403 Version 1 (Component)
PLRV Coat Protein gene - Isolated from Desir??e potato

pabA
BBa_K137005 Version 1 (Component)
pabA (from pABA synthesis)

BBa_K2088001
BBa_K2088001 Version 1 (Component)
copA promoter

BBa_R0001
BBa_R0001 Version 1 (Component)
HMG-CoA Dependent RBS Blocking Segment

dCas 9
BBa_K1051800 Version 1 (Component)
protein dCas9.Two mutations,D10A and H841A,from Cas9 gene without stop codon.

BBa_K654071
BBa_K654071 Version 1 (Component)
psbA2 controlled Fatty Acyl-CoA Synthetase (Ligase)

CMV AID pA
BBa_K929000 Version 1 (Component)
AID with CMV promoter and hGH-polyadenylation signal sequence

BBa_K218001
BBa_K218001 Version 1 (Component)
LuxOU from Vibrio harveyi