BBa_J22136
BBa_J22136 Version 1 (Component)
The mRFP gene under the Rec A(SOS) promoter

BBa_I714030
BBa_I714030 Version 1 (Component)
OriT-F (Origin of Transfer for the F-plasmid nic region)

BBa_K1861020
BBa_K1861020 Version 1 (Component)
Fusion protein of the TALE constant domain and a SpyTag in an expression cassette

BBa_C0099
BBa_C0099 Version 1 (Component)
cviR regulatory protein from Chromobacterium Violaceum

BBa_K1362060
BBa_K1362060 Version 1 (Component)
OmpA surface protein as RFC[105] N-terminal insert

SCVE
BBa_K1593666 Version 1 (Component)
SARS Coronavirus Envelope Protein

BBa_K1316001
BBa_K1316001 Version 1 (Component)
mKate2 protein with double transcription terminator

BBa_K2082209
BBa_K2082209 Version 1 (Component)
Fusion protein Gal11P:cI(434)

BBa_K1179009
BBa_K1179009 Version 1 (Component)
(Cas9-VP16) A non-nuclease Cas9 with a C-terminal VP16 fusion for activation

BBa_K1217009
BBa_K1217009 Version 1 (Component)
Localizing polyphosphate kinase (ppk1) into Histag modified Eut microcompartment (BBa_K311004)

BBa_M36624
BBa_M36624 Version 1 (Component)
Antifungal protein from Aspergillus giganteus (active)

BBa_K777001
BBa_K777001 Version 1 (Component)
Tar receptor under the control of constitutive promoter J23100

BBa_K1159105
BBa_K1159105 Version 1 (Component)
Mature Nuclease NucA from <i>Staphylococcus aureus</i> (Thermonuclease) in RFC[25]

BBa_M1538
BBa_M1538 Version 1 (Component)
Antifreeze protein - YL2 variant

BBa_K258015
BBa_K258015 Version 1 (Component)
Vhb protein

BBa_K1315005
BBa_K1315005 Version 1 (Component)
The <i>clp</i> gene from <i>Xanthomonas campestris</i>

BBa_K1315003
BBa_K1315003 Version 1 (Component)
The <i>rpfG</i> gene from <i>Xanthomonas campestris</i>

BBa_K371024
BBa_K371024 Version 1 (Component)
10*GS linker(for RFC 53 protein fusion)

BBa_K1658004
BBa_K1658004 Version 1 (Component)
Limonene Synthase LS

BBa_J29005
BBa_J29005 Version 1 (Component)
PoPs->CrtI protein generator

BBa_J29004
BBa_J29004 Version 1 (Component)
PoPS ->CrtB protein generator

BBa_I11024
BBa_I11024 Version 1 (Component)
lambda Int composite

BBa_K931010
BBa_K931010 Version 1 (Component)
Red Fluorescent Protein Domain -Starch Binding Protein Domain

BBa_K831009
BBa_K831009 Version 1 (Component)
Inducible HipB antitoxin under the control of lac promoter

BBa_J03210
BBa_J03210 Version 1 (Component)
malE [Maltose-binding Protein]

BBa_K2082208
BBa_K2082208 Version 1 (Component)
Fusion protein Gal4:RpoZ

BBa_J64019
BBa_J64019 Version 1 (Component)
invI SPI-1 Protein

BBa_K2082201
BBa_K2082201 Version 1 (Component)
Fusion protein HA4:cMyc:RpoZ

BBa_K1725302
BBa_K1725302 Version 1 (Component)
Member of the RBS library - derived from BBa_B0032

BBa_K1054000
BBa_K1054000 Version 1 (Component)
protein E of Phage phiX174

Smt3-mDNMT
BBa_K1362500 Version 1 (Component)
Smt3-Murine DNMT1(731-1602) fusion protein (with His6)

BBa_K396011
BBa_K396011 Version 1 (Component)
LuxR protein generator

BBa_K1510016
BBa_K1510016 Version 1 (Component)
red florescence protein RFC23

BBa_K1315006
BBa_K1315006 Version 1 (Component)
The <i>manA</i> promoter from <i>Xanthomonas campestris</i>

BBa_M1891
BBa_M1891 Version 1 (Component)
green fluorescent protein

TetR-RBS-G
BBa_K079004 Version 1 (Component)
TetR protein with GFP reporter protein

CI-RBS-GFP
BBa_K079009 Version 1 (Component)
CI protein with GFP reporter protein

LacI-RBS-G
BBa_K079001 Version 1 (Component)
LacI protein with GFP reporter protein

BBa_J16102
BBa_J16102 Version 1 (Component)
HngR - Gene encodes Hunger protein

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K339001
BBa_K339001 Version 1 (Component)
Mutant Maltose Binding Protein (malE31)

BBa_K515100
BBa_K515100 Version 1 (Component)
IAA biosynthetic genes under control of the Pveg2 promoter

BBa_I10009
BBa_I10009 Version 1 (Component)
Repressor Protein

BBa_K364166
BBa_K364166 Version 1 (Component)
Zinc Finger Protein fixed C-terminus

BBa_K364165
BBa_K364165 Version 1 (Component)
Zinc Finger Protein fixed N-terminus

BBa_K331008
BBa_K331008 Version 1 (Component)
Outer Membrane Protein OmpA

BBa_K258014
BBa_K258014 Version 1 (Component)
Vitreoscilla hemoglobin (VHb) protein

BBa_K1113400
BBa_K1113400 Version 1 (Component)
Targeting sequence for the delivery of the LacZ gene to the Carboxysome.

BBa_K1113410
BBa_K1113410 Version 1 (Component)
Targeting sequence for the delivery of the LacZ gene to the Carboxysome.