BBa_K185033BBa_K185033 Version 1 (Component)An inverter of a special lactose operon system based on J23110-rbs34-lacI-dter-plac-rbs31
BBa_K1361004BBa_K1361004 Version 1 (Component)Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag
BBa_K2082237BBa_K2082237 Version 1 (Component)RFP and Tetracycline resistence(tetA) under the control of a modified lacZ promoter; Fusion protein
BBa_K1994060BBa_K1994060 Version 1 (Component)Multiple dCas9 binding sites with weak sigma 54 promoter upstream of GFP + dCas9 expression cassette
pCMV-ECFP-BBa_I763023 Version 1 (Component)LacI coding device with ECFP as a reporter regulated by pCMV
BBa_K079049BBa_K079049 Version 1 (Component)GFP reporter protein under the control of the J23118 constitutive promoter and LexA 2 operator
Altered Sequence ActivitiesAlteredSequenceActivities_collection Version 1 (Collection)A set of activities to point to ways in which sequences have been altered between source and upload
BBa_K079020BBa_K079020 Version 1 (Component)GFP reporter under the control of J23118 promoter and Lac 2 operator auto-regulated by LacI protein
BBa_K1109007BBa_K1109007 Version 1 (Component)FepA L8T Mutant -composite of Large Diffusion pore for E. coli outer membrane coding sequence transc
BBa_K1778005BBa_K1778005 Version 1 (Component)eGFP:enhanced Green Fluorescent Protein. It???s the mutant of GFP. It is widely used as report gene
BBa_J31016BBa_J31016 Version 1 (Component)part produces the RNA construct crRNA-RBS-GFPLVA-tt that can only be translated in the presence of t
BBa_K2088006BBa_K2088006 Version 1 (Component)It encodes a kind of protein named 2Fe-2S ferredoxin, a 2Fe-2S iron-sulfur cluster binding domain. I
Bm3R1BBa_K1401000 Version 1 (Component)TetR homolog. This is a MoClo part of the Bm3R1 repressor gene with CD fusion sites ('AATG', 'AGGT')
BBa_K1641024BBa_K1641024 Version 1 (Component)Reporter of Invertase activity of Cre, pInv-rep-100LoxM
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_K542012BBa_K542012 Version 1 (Component)Catechol 2,3-dioxygenase with a C-terminal Arg-tag and Double Terminator (no promoter/RBS)
BBa_K1069001BBa_K1069001 Version 1 (Component)-- No description --
BBa_S03736BBa_S03736 Version 1 (Component)pLac-lox-RBS-Tet (in pSB1A2)
BBa_K1189029BBa_K1189029 Version 1 (Component)TALE-A with a his tag linked to a K coil under the control of a LacI promoter
BBa_I763000BBa_I763000 Version 1 (Component)-- No description --
BBa_M45102BBa_M45102 Version 1 (Component)Cobalt detection Biobrick. RFP made in presence of Cobalt. Note the receptor also works in the prese
BBa_K737000BBa_K737000 Version 1 (Component)We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig
BBa_M31513BBa_M31513 Version 1 (Component)Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III
BBa_M31516BBa_M31516 Version 1 (Component)Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III
YFP_SPferBBa_K809314 Version 1 (Component)YFP + signal peptide of FER
BBa_J04431BBa_J04431 Version 1 (Component)GFP Coding Device with promoter, RBS, GFP with LVA tag, and Terminator
BBa_K987000BBa_K987000 Version 1 (Component)This part is a coding part that produces Vip3Ca3, a protein that can deals with different forms of p
BBa_M30002BBa_M30002 Version 1 (Component)-- No description --
BBa_M30004BBa_M30004 Version 1 (Component)-- No description --
BBa_K2184029BBa_K2184029 Version 1 (Component)SNP for non taster NaCl & Sour -TAS1R1 rs17492553
BBa_K189060BBa_K189060 Version 1 (Component)Gp15 of Bacteriophage Mu
BBa_J06652BBa_J06652 Version 1 (Component)Test of BBa_R0010
BBa_M11085BBa_M11085 Version 1 (Component)E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o
BBa_K737001BBa_K737001 Version 1 (Component)We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig
BBa_M30005BBa_M30005 Version 1 (Component)-- No description --
BBa_M30008BBa_M30008 Version 1 (Component)-- No description --
BBa_M30007BBa_M30007 Version 1 (Component)-- No description --
BBa_M30010BBa_M30010 Version 1 (Component)-- No description --
BBa_K1676120BBa_K1676120 Version 1 (Component)Mutant 16 of Lactate Dehydrogenase
BBa_K1676114BBa_K1676114 Version 1 (Component)Mutant 10 of Lactate Dehydrogenase
BBa_K1361007BBa_K1361007 Version 1 (Component)Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative