BBa_J06551
BBa_J06551 Version 1 (Component)
Construction intermediate: lambda cI with RBS and hybrid LacI promoter (R0011.B0034.C0051)

BBa_J04795
BBa_J04795 Version 1 (Component)
Riboswitch designed to turn "ON" a protein

BBa_I0414
BBa_I0414 Version 1 (Component)
RBS Test R0040.B0032.E0030.B0015 RBS Test

BBa_J58008
BBa_J58008 Version 1 (Component)
Periplasmic binding protein that docks a vanillin molecule

BBa_K091119
BBa_K091119 Version 1 (Component)
LacI protein generator with a pTet promoter

BBa_K783048
BBa_K783048 Version 1 (Component)
This is a MoClo converted version of BBa_B0031

BBa_K783043
BBa_K783043 Version 1 (Component)
This is a MoClo converted version of BBa_J23102

BBa_K783039
BBa_K783039 Version 1 (Component)
This is a MoClo converted version of BBa_J23101

BBa_K783049
BBa_K783049 Version 1 (Component)
This is a MoClo converted version of BBa_B0032

BBa_K783040
BBa_K783040 Version 1 (Component)
This is a MoClo converted version of BBa_J23110

BBa_K1413045
BBa_K1413045 Version 1 (Component)
A fusion of Universal Transposon Plasmid and pSB1C3

BBa_K783034
BBa_K783034 Version 1 (Component)
This is a MoClo converted version of BBa_J23114

BBa_K783051
BBa_K783051 Version 1 (Component)
This is a MoClo converted version of BBa_B0034

BBa_K783047
BBa_K783047 Version 1 (Component)
This is a MoClo converted version of BBa_B0030

BBa_K783050
BBa_K783050 Version 1 (Component)
This is a MoClo converted version of BBa_B0033

iGEM Parts Registry
igem_collection Version 1 (Collection)
The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.

placIQ RBS
BBa_K193604 Version 1 (Component)
GFP behind a constitutive promoter (placIQ) on pSB4A5

BBa_K594012
BBa_K594012 Version 1 (Component)
a ECFP reporter having RBS and double terminator

BBa_K1405007
BBa_K1405007 Version 1 (Component)
A Kill Switch with "memory" time repressed by IPTG

BBa_K812130
BBa_K812130 Version 1 (Component)
Citrine reporter with a Kozak sequence for expression in Xenopus

BBa_K2092004
BBa_K2092004 Version 1 (Component)
alcR (incl RBS), ethanol-activated transcription factor from A. nidulans

BBa_K346025
BBa_K346025 Version 1 (Component)
PmerT promoter mutant 88+RBS(B0030)+GFP(E0040)Terminator(B0010)+Terminator(B0012)

BBa_K539521
BBa_K539521 Version 1 (Component)
heat sensitive cI QPI with high promoter and a RBS

pCMV-ECFP-
BBa_I763023 Version 1 (Component)
LacI coding device with ECFP as a reporter regulated by pCMV

BBa_J58011
BBa_J58011 Version 1 (Component)
Promoter which is activated by cI and CRP, using a transcription logic function type AND

BBa_K1796201
BBa_K1796201 Version 1 (Component)
An unloaded sgRNA that contains BbsI cutting site, with a promoter and terminator.

BBa_K300096
BBa_K300096 Version 1 (Component)
Double phasin and intein separed by a flexible protein domain linker

BBa_K079016
BBa_K079016 Version 1 (Component)
RecA promoter with GFP reporter protein on a medium copy number plasmid

BBa_J22121
BBa_J22121 Version 1 (Component)
Lac Y gene under the rec A(SOS) promoter in plasmid pSB2K3

BBa_K371054
BBa_K371054 Version 1 (Component)
MPF(meta-prefix)+[GFP+10*GS+A] fusion protein+MSF(meta-suffix))

BBa_K1412088
BBa_K1412088 Version 1 (Component)
A combination of theophylline aptamer and taRNA that can response theophylline to regulate circuit

BBa_R4030
BBa_R4030 Version 1 (Component)
PoPS/RiPS Generator composed of the Tet promoter and a strong RBS (R0040.E0030)

BBa_K1778002
BBa_K1778002 Version 1 (Component)
TRE-CYC1TATA is a recombinant promoter, which is constructed in order to make the Tet-on system func

BBa_K1942001
BBa_K1942001 Version 1 (Component)
This part is a short RNA sequence designed for KRAS gene silencing. It is used for down-regulating K

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.