BBa_K1510103
BBa_K1510103 Version 1 (Component)
MicC scaffold from E.coli MG1655

BBa_K1497027
BBa_K1497027 Version 1 (Component)
Scaffold (G-P)

BBa_K1497028
BBa_K1497028 Version 1 (Component)
Scaffold (P-P)

BBa_K1497029
BBa_K1497029 Version 1 (Component)
Scaffold (S-P)

BBa_K1497030
BBa_K1497030 Version 1 (Component)
Scaffold (S-S)

BBa_K1497031
BBa_K1497031 Version 1 (Component)
Scaffold (G-S-P-His)

BBa_K1497032
BBa_K1497032 Version 1 (Component)
Scaffold (G-S-P-His-Cys)

BBa_K1497033
BBa_K1497033 Version 1 (Component)
Scaffold (G-S-P)

BBa_K1329002
BBa_K1329002 Version 1 (Component)
Insertion of the Metallothionein Cup-1-1 domain into Bacillus subtilis flagellin

BBa_K1431201
BBa_K1431201 Version 1 (Component)
NLS, lead protein into the nucleus

BBa_K1831002
BBa_K1831002 Version 1 (Component)
T3-10 Self-Assembling Scaffold with K-coil & His tag

His-Scaffo
BBa_K1602027 Version 1 (Component)
Protein scaffold with N-terminal HisTag

RBS-Scaffo
BBa_K1602028 Version 1 (Component)
Protein scaffold with N-terminal HisTag and RBS BBa_B0034

Scaffold g
BBa_K1602029 Version 1 (Component)
Inducible generator of protein scaffold with N-terminal HisTag

His-Sc_Si4
BBa_K1602030 Version 1 (Component)
HisTag-Scaffold_Si4-Tag fusion protein

RBS-His-Sc
BBa_K1602031 Version 1 (Component)
HisTag-Scaffold_Si4-Tag fusion protein with RBS BBa_B0034

Sc-Si4 gen
BBa_K1602032 Version 1 (Component)
Inducible generator of HisTag-Scaffold_Si4-Tag fusion protein

BBa_K1979010
BBa_K1979010 Version 1 (Component)
Promoter (BBa_J23101)+PBP5+T7 terminator

BBa_K1979003
BBa_K1979003 Version 1 (Component)
PBP5 coding device (promoter+PBP5+T7 terminator)

BBa_K2066520
BBa_K2066520 Version 1 (Component)
F30 scaffold / 2xBroccoli

BBa_K2066050
BBa_K2066050 Version 1 (Component)
F30 scaffold / 2x Broccoli

BBa_K2155012
BBa_K2155012 Version 1 (Component)
Synthetic Protein Scaffold

BBa_K2140002
BBa_K2140002 Version 1 (Component)
StarScaffold Prototype 1

BBa_K2140003
BBa_K2140003 Version 1 (Component)
StarScaffold Prototype 2

BBa_K2140004
BBa_K2140004 Version 1 (Component)
StarScaffold Double Helix 1

BBa_K1937002
BBa_K1937002 Version 1 (Component)
OriKan: a composite part to turn any pSB1C3 into a <i>Bacillus</i> plasmid

BBa_K1934030
BBa_K1934030 Version 1 (Component)
Streptavidin with CBD_CipA (cellulosomal scaffolding protein A)

BBa_K2066536
BBa_K2066536 Version 1 (Component)
Spinach Aptamer w/ tRNA Scaffold

BBa_K1923002
BBa_K1923002 Version 1 (Component)
Scaffold msr-Scaffold msd-Reverse transcriptase encoding gene

BBa_K2109103
BBa_K2109103 Version 1 (Component)
&#955;cl-Nb (Scaffold)

Digitalizer
Digitalizer_collection Version 1 (Collection)
A genetic device to digitalize gene expression into a sharp on/off signal.

BBa_M12100
BBa_M12100 Version 1 (Component)
PLambda : B0034 : RFP in BioScaffold Form, a (0,5;7,2] BioScaffold part

BBa_M12101
BBa_M12101 Version 1 (Component)
PTet : B0034 : GFP in BioScaffold Form, a [0,5;7,2) BioScaffold

BBa_J70663
BBa_J70663 Version 1 (Component)
Left BioScaffold part for Grant's project

BBa_M1051
BBa_M1051 Version 1 (Component)
DNA Scaffold for Binding Domains

BBa_M1055
BBa_M1055 Version 1 (Component)
DNA Scaffold for Binding Domains

BBa_M1026
BBa_M1026 Version 1 (Component)
DNA Scaffold for Binding Domains

BBa_K656002
BBa_K656002 Version 1 (Component)
Cohesin region of Scaffolding Protein A from Clostridium thermocellum

BBa_K656009
BBa_K656009 Version 1 (Component)
C. cellulolyticum scaffoldin linker

BBa_K581008
BBa_K581008 Version 1 (Component)
RBS+ccdB+Termintor

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K112245
BBa_K112245 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!} inserted into K112995 plasmid

BBa_K112246
BBa_K112246 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112995 plasmid

BBa_K112247
BBa_K112247 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!} inserted into K112950 plasmid

BBa_K112248
BBa_K112248 Version 1 (Component)
{pAH57 promoter}{rbs.xis.int!}{rbs.ihfA!}{rbs.ihfB!} inserted into K112950 plasmid

BBa_K146001
BBa_K146001 Version 1 (Component)
AOX1 ethanol sensitive promoter cloned into RFP vector BBa_J61002

BBa_J70030
BBa_J70030 Version 1 (Component)
A BioScaffold Part (Uses PpiI), Protein Tail Remover see Part Design Page

BBa_J70031
BBa_J70031 Version 1 (Component)
Linker for BioScaffold Parts, example of Gamma BioScaffold part