IBMc388
IBMc388 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-174)-GGG-M86N-cat-Plux2-B32-M86C-S-mCherry*(175-236)-H6-T

IBMc389
IBMc389 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-176)-HYG-M86N-cat-Plux2-B32-M86C-S-mCherry*(177-236)-H6-T

IBMc390
IBMc390 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-185)-YNG-M86N-cat-Plux2-B32-M86C-S-mCherry*(186-236)-H6-T

IBMc398
IBMc398 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-187)-ANG-M86N-cat-Plux2-B32-M86C-S-mCherry*(188-236)-H6-T

IBMc399
IBMc399 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-188)-KNG-M86N-cat-Plux2-B32-M86C-S-mCherry*(189-236)-H6-T

IBMc400
IBMc400 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-190)-PVG-M86N-cat-Plux2-B32-M86C-S-mCherry*(191-236)-H6-T

IBMc401
IBMc401 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-191)-VHG-M86N-cat-Plux2-B32-M86C-S-mCherry*(192-236)-H6-T

IBMc402
IBMc402 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-QLG-M86N-cat-Plux2-B32-M86C-S-mCherry*(193-236)-H6-T

IBMc403
IBMc403 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-194)-PGG-M86N-cat-Plux2-B32-M86C-S-mCherry*(195-236)-H6-T

BBa_K424006
BBa_K424006 Version 1 (Component)
Rhamnosiltransferase 1 gene (rhlAB)

rhlAB
BBa_K424018 Version 1 (Component)
Rhamnosiltransferase BioBrick (Rh1AB_BB)

BBa_K424017
BBa_K424017 Version 1 (Component)
Test plataform for rhamnosyltransferase BioBrick (Rh1AB_BB) expression in E. coli

BBa_K395603
BBa_K395603 Version 1 (Component)
alcohol acetyltransferase; converts butanol or 2-methylbutanol to butyl acetate or 2-methylbutyl ace

BBa_M36006
BBa_M36006 Version 1 (Component)
glucose1phosphate adenylyltransferase

BSMT1
BBa_J45004 Version 1 (Component)
SAM:benzoic acid/salicylic acid carboxyl methyltransferase I; converts salicylic acid to methyl sali

BBa_J100017
BBa_J100017 Version 1 (Component)
TT+pLux+RBS+LuxI(2-SAT 2 clause)+RBS+GFP+pLac+RBS+LuxR+tRNAs

BBa_J85003
BBa_J85003 Version 1 (Component)
3OC6HSL -> with non-functional RBS, RFP

BBa_J85004
BBa_J85004 Version 1 (Component)
3OC6HSL -> non-functional RBS, RFP; constitutive GFP

BBa_J85005
BBa_J85005 Version 1 (Component)
3OC6HSL -> non-functional RBS, RFP, terminator

BBa_J85006
BBa_J85006 Version 1 (Component)
3OC6HSL -> non-functional RBS, RFP, terminator; constitutive GFP

BBa_K549020
BBa_K549020 Version 1 (Component)
glutamine aminotransferase

mdnED
BBa_K627000 Version 1 (Component)
ABC transporter and N-acetyltransferase from mdn-cluster

mdnD
BBa_K627004 Version 1 (Component)
N-acetyltransferase from the mdn-cluster

BBa_K654096
BBa_K654096 Version 1 (Component)
E. coli Reference Promoter (fusion of Anderson 0.51 and 1.00 promoters)

BBa_K640002
BBa_K640002 Version 1 (Component)
oriT - Interspecies origin of transfer

BBa_K581015
BBa_K581015 Version 1 (Component)
pBAD-supD-plux_inv-T7ptag

BBa_K764023
BBa_K764023 Version 1 (Component)
Cyclodextrin glycosyltransferase

BBa_K764026
BBa_K764026 Version 1 (Component)
Cyclodextrin glycosyltransferase + BBa_B0014

BBa_K764030
BBa_K764030 Version 1 (Component)
BBa_J13002 + cyclodextrin glycosyltransferase + BBa_B0014

BBa_K784013
BBa_K784013 Version 1 (Component)
pLux+Theophylline riboswitch+mCherry

BBa_K784014
BBa_K784014 Version 1 (Component)
pLux+Spacer2+RBS+mCherry

BBa_K934022
BBa_K934022 Version 1 (Component)
Plux-LasI

BBa_K934024
BBa_K934024 Version 1 (Component)
Plux/tet hybrid promoter

BBa_K874000
BBa_K874000 Version 1 (Component)
M.ScaI Methyltransferase

BBa_K934025
BBa_K934025 Version 1 (Component)
Plux/tet-GFP

BBa_K934026
BBa_K934026 Version 1 (Component)
Plux-LacI

DGAT
BBa_K836001 Version 1 (Component)
O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus)

DGAT
BBa_K836002 Version 1 (Component)
O-acyltransferase WSD from Acinetobacter sp. (codon usage optimized for R. opacus) as used

BBa_K873003
BBa_K873003 Version 1 (Component)
conjugative transfer of RFG

BBa_K873013
BBa_K873013 Version 1 (Component)
cujugative transfer death gene

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

OriTR
BBa_J01003 Version 1 (Component)
OriT-R (Origin of transfer for the R-plasmid nic region)

BBa_K156011
BBa_K156011 Version 1 (Component)
aadA (streptomycin 3'-adenyltransferase)

Bioplastic
BBa_K156012 Version 1 (Component)
phaA (acetyl-CoA acetyltransferase)

BBa_K116617
BBa_K116617 Version 1 (Component)
pLux + BBa_E0240

BBa_K116637
BBa_K116637 Version 1 (Component)
pLux + RBS + CII + T

BBa_K116658
BBa_K116658 Version 1 (Component)
pLux tester