BBa_J120030
BBa_J120030 Version 1 (Component)
Composite part with Yeast promoter,CH1 domain of human Ab, ADAM17 cleavage sequence & Phoratoxin.

BBa_K1363604
BBa_K1363604 Version 1 (Component)
key of no of RNA logic gates

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1861020
BBa_K1861020 Version 1 (Component)
Fusion protein of the TALE constant domain and a SpyTag in an expression cassette

BBa_K079021
BBa_K079021 Version 1 (Component)
LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La

BBa_K1994022
BBa_K1994022 Version 1 (Component)
Multiple dCas9 binding sites with weak omega promoter upstream of GFP + dCas9 expression cassette

BBa_K2055015
BBa_K2055015 Version 1 (Component)
Promoter + Inverter with Ferric Uptake Regulator & LacI. Activates gene expression in presence of Fe

BBa_M45101
BBa_M45101 Version 1 (Component)
Composite biobrick intended to induce biofilm formation in the presence of lactose. Made from parts

LaLaLa
BBa_K1479001 Version 1 (Component)
0.0 Testing the UE of the bio-brick upload page, not designing a real one

BBa_K2055019
BBa_K2055019 Version 1 (Component)
Part designed for verification and characterization of the heat the shock promoter and Inverter.

BBa_K880001
BBa_K880001 Version 1 (Component)
Asymmetrically digestible reporter to assay the activity of DNA recombinases FimE K137007 and HbiF K

BBa_K2176001
BBa_K2176001 Version 1 (Component)
Cassette for the constitutive production of GAL4-KaiCp and LexA-SasA, linked by a self-cleaving P2A

BBa_K1216014
BBa_K1216014 Version 1 (Component)
BBa_K1216014 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL.

BBa_K1216012
BBa_K1216012 Version 1 (Component)
BBa_K1216012 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL.

BBa_K1216013
BBa_K1216013 Version 1 (Component)
BBa_K1216013 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL.

BBa_K1216011
BBa_K1216011 Version 1 (Component)
BBa_K1216007 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL.

BBa_K1216010
BBa_K1216010 Version 1 (Component)
BBa_K1216010 is a variant of the wild-type luxR promoter with a lowered sensitivity for LuxR-AHL. (

BBa_K1361004
BBa_K1361004 Version 1 (Component)
Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag

BBa_K2082237
BBa_K2082237 Version 1 (Component)
RFP and Tetracycline resistence(tetA) under the control of a modified lacZ promoter; Fusion protein

BBa_K1994060
BBa_K1994060 Version 1 (Component)
Multiple dCas9 binding sites with weak sigma 54 promoter upstream of GFP + dCas9 expression cassette

BBa_K079049
BBa_K079049 Version 1 (Component)
GFP reporter protein under the control of the J23118 constitutive promoter and LexA 2 operator

Altered Sequence Activities
AlteredSequenceActivities_collection Version 1 (Collection)
A set of activities to point to ways in which sequences have been altered between source and upload

BBa_K079020
BBa_K079020 Version 1 (Component)
GFP reporter under the control of J23118 promoter and Lac 2 operator auto-regulated by LacI protein

BBa_K1109007
BBa_K1109007 Version 1 (Component)
FepA L8T Mutant -composite of Large Diffusion pore for E. coli outer membrane coding sequence transc

BBa_K1778005
BBa_K1778005 Version 1 (Component)
eGFP:enhanced Green Fluorescent Protein. It???s the mutant of GFP. It is widely used as report gene

BBa_M11410
BBa_M11410 Version 1 (Component)
Type 2 promoter of sigE gene. Sigma factor regulates light and nitrogen responses, and has been obse

BBa_J31016
BBa_J31016 Version 1 (Component)
part produces the RNA construct crRNA-RBS-GFPLVA-tt that can only be translated in the presence of t

Bm3R1
BBa_K1401000 Version 1 (Component)
TetR homolog. This is a MoClo part of the Bm3R1 repressor gene with CD fusion sites ('AATG', 'AGGT')

Devices from the iGEM 2016 interlab
iGEM_2016_interlab_collection Version 1 (Collection)
This is a collection of devices that were used in the 2016 iGEM interlab study

BBa_K1189029
BBa_K1189029 Version 1 (Component)
TALE-A with a his tag linked to a K coil under the control of a LacI promoter

BBa_M45102
BBa_M45102 Version 1 (Component)
Cobalt detection Biobrick. RFP made in presence of Cobalt. Note the receptor also works in the prese

BBa_K1799017
BBa_K1799017 Version 1 (Component)
NsrR open reading frame

BBa_K737000
BBa_K737000 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_M31513
BBa_M31513 Version 1 (Component)
Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III

BBa_M31516
BBa_M31516 Version 1 (Component)
Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III

YFP_SPfer
BBa_K809314 Version 1 (Component)
YFP + signal peptide of FER

BBa_K987000
BBa_K987000 Version 1 (Component)
This part is a coding part that produces Vip3Ca3, a protein that can deals with different forms of p

BBa_I13035
BBa_I13035 Version 1 (Component)
3OC₆HSL Receiver Device with Inducible Control of LuxR and a YFP Output device

BBa_K189060
BBa_K189060 Version 1 (Component)
Gp15 of Bacteriophage Mu

BBa_M11085
BBa_M11085 Version 1 (Component)
E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o

BBa_K737001
BBa_K737001 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig