BBa_M1529
BBa_M1529 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP

BBa_M1541
BBa_M1541 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL3) with 8-His tag with secretion tag and GFP

BBa_M1531
BBa_M1531 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP

PrtDEF
BBa_K258008 Version 1 (Component)
Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi

BBa_M1542
BBa_M1542 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL3) with 8-His tag with secretion tag and GFP

BBa_M1535
BBa_M1535 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP

BBa_K2150029
BBa_K2150029 Version 1 (Component)
pCon+RBS+tetR+DT+pTet+RBS+T7RNAP+Ter+RBS+pT7+tetX-GFP(fusion protein)+DT

BBa_M1536
BBa_M1536 Version 1 (Component)
Thermal Hysteresis (Antifreeze) Protein (variant YL4) with 8-His tag with secretion tag and GFP

BBa_K563010
BBa_K563010 Version 1 (Component)
Tor2 gene from the genome of the S. cerevisiae, central protein in TOR(target of rapamycin) pathway

BBa_K2027009
BBa_K2027009 Version 1 (Component)
Control Sequence Peptide for Melanin Binding (Nonpolar-Protic)

BBa_K1051116
BBa_K1051116 Version 1 (Component)
Actin Targeting Protein

BBa_M1174
BBa_M1174 Version 1 (Component)
protein1

BBa_K1790001
BBa_K1790001 Version 1 (Component)
biosensor detect food allergens. A proteins conformation change in response to ligand binding couple

BBa_K1790005
BBa_K1790005 Version 1 (Component)
biosensor detect food allergens. A proteins conformation change in response to ligand binding couple

BBa_K1790000
BBa_K1790000 Version 1 (Component)
biosensors detect food allergens. A proteins conformation change in response to ligand binding coupl

BBa_K1790003
BBa_K1790003 Version 1 (Component)
biosensor detect food allergens. A proteins conformation change in response to ligand binding couple

BBa_K1790004
BBa_K1790004 Version 1 (Component)
biosensor detect food allergens. A proteins conformation change in response to ligand binding couple

BBa_K1790002
BBa_K1790002 Version 1 (Component)
biosensor detect food allergens. A proteins conformation change in response to ligand binding couple

PcotYZ
BBa_K823030 Version 1 (Component)
P_<i>cotYZ</i>: <i>B. subtilis</i> promoter regulating expression of spore crust proteins

BBa_M45091
BBa_M45091 Version 1 (Component)
AGA1: Agglutinins, mating type specific cell surface Proteins, are synthesized by haploid cell of Sa

BBa_K2018034
BBa_K2018034 Version 1 (Component)
phaCAB genes with additional promoter and RBS, that encodes proteins that can create PHB.

BBa_K2018000
BBa_K2018000 Version 1 (Component)
phaCAB genes with additional promoter and RBS, that encodes proteins that can create PHB.

BBa_K2018036
BBa_K2018036 Version 1 (Component)
phaCAB genes with additional promoter and RBS, that encodes proteins that can create PHB.

BBa_K2018033
BBa_K2018033 Version 1 (Component)
phaCAB genes with additional promoter and RBS, that encodes proteins that can create PHB.

BBa_K987000
BBa_K987000 Version 1 (Component)
This part is a coding part that produces Vip3Ca3, a protein that can deals with different forms of p

GFP-AID
BBa_K812110 Version 1 (Component)
GFP fusion with the ubuquitinase E3 OsTirI recoginition domain for PSC2+ plasmid

enhanced
BBa_K2075011 Version 1 (Component)
enhanced Green fluorescenc protein (GFP)

enhanced G
BBa_K2075012 Version 1 (Component)
enhanced Green fluorescenc protein (GFP)

BBa_J58009
BBa_J58009 Version 1 (Component)
Fusion protein Trg-EnvZ

BBa_M11085
BBa_M11085 Version 1 (Component)
E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o

BBa_K1123017
BBa_K1123017 Version 1 (Component)
DNA Coding Sequence for EGFP Protein

BBa_I721004
BBa_I721004 Version 1 (Component)
Lead Binding Protein + Promoter (version a)

BBa_K1014016
BBa_K1014016 Version 1 (Component)
HrpS Protein Generator

BBa_K125550
BBa_K125550 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_K079021
BBa_K079021 Version 1 (Component)
LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_J133568
BBa_J133568 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133564
BBa_J133564 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133573
BBa_J133573 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133563
BBa_J133563 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133572
BBa_J133572 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133579
BBa_J133579 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133566
BBa_J133566 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133570
BBa_J133570 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133569
BBa_J133569 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133578
BBa_J133578 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133580
BBa_J133580 Version 1 (Component)
Protein Linker SSGSSG Library

BBa_J133565
BBa_J133565 Version 1 (Component)
Protein Linker SSGSSG Library