pLacIQ1-RB
BBa_K193401 Version 1 (Component)
cph8 under the control of Plac

CRP
BBa_J58112 Version 1 (Component)
CRP protein

BBa_J61112
BBa_J61112 Version 1 (Component)
Ribosome Binding Site Family Member

BBa_K539641
BBa_K539641 Version 1 (Component)
a RBS (B0032)+ilvC with a RBS (B0032)+ilvD

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1431301
BBa_K1431301 Version 1 (Component)
TRE-3G promoter+SV40 PolyA, an ideal controller of mammalian gene expression with Tet-On 3G protein

BBa_K173019
BBa_K173019 Version 1 (Component)
adhB protein generator

RBS-cph8
BBa_K193405 Version 1 (Component)
RBS is located upstream of cph8

BBa_K1325016
BBa_K1325016 Version 1 (Component)
small RNAs regulatory system

BBa_K1072015
BBa_K1072015 Version 1 (Component)
GAL1 + Brho +Flag + odr-10 +GFP +ADH1

BBa_K1431201
BBa_K1431201 Version 1 (Component)
NLS, lead protein into the nucleus

BBa_K1332005
BBa_K1332005 Version 1 (Component)
The 3' side of the intron(+exon fragment) from td gene of T4 phage

BBa_K2027010
BBa_K2027010 Version 1 (Component)
Repetitive Insertion of 4D Melanin-Binding Heptapeptide

BBa_K1736000
BBa_K1736000 Version 1 (Component)
Harmonised B. subtilis Falvin Binding Fluorescent Protein (BsFP)

BBa_K310014
BBa_K310014 Version 1 (Component)
GolB (gold-binding protein) with RBS and terminator

BBa_K1033239
BBa_K1033239 Version 1 (Component)
Signal peptide from Lactobacillus reuteri collagen binding protein

BBa_K1947016
BBa_K1947016 Version 1 (Component)
The test of the Pmsp3 promoter.

BBa_K1947015
BBa_K1947015 Version 1 (Component)
The test of the Pmsp3 promoter.

BBa_J100029
BBa_J100029 Version 1 (Component)
The promoter of rpoDPhs

AraC_14_3C
BBa_K627011 Version 1 (Component)
Fusion part of pBAD arabinose-inducible induction system and the HRV 14_3C protease

BBa_K539621
BBa_K539621 Version 1 (Component)
a RBS (B0030)+ilvC

epsE 5 IS
BBa_K143005 Version 1 (Component)
5??? Integration Sequence for the epsE locus of B. subtilis

epsE 3 IS
BBa_K143006 Version 1 (Component)
3??? Integration Sequence for the epsE locus of B. subtilis

BBa_K156009
BBa_K156009 Version 1 (Component)
OFP (orange fluorescent protein)

BBa_K1151010
BBa_K1151010 Version 1 (Component)
GFP nickel regulated 2

PalkM-tetA
BBa_K1020010 Version 1 (Component)
TetA gene under the control of PalkM

BBa_K189042
BBa_K189042 Version 1 (Component)
Integration protein of lambda genome

BBa_K812012
BBa_K812012 Version 1 (Component)
OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin

BBa_K574006
BBa_K574006 Version 1 (Component)
3OC12HSL regulated by pTet and pBad

BBa_K153008
BBa_K153008 Version 1 (Component)
CqsA - the CAI-1 producer

BBa_K817036
BBa_K817036 Version 1 (Component)
J23119-RBS-SP3-Penetratin-Flag-xx

BBa_K817035
BBa_K817035 Version 1 (Component)
J23119-RBS-SP2-Penetratin-Flag-xx

LacIm
BBa_K092800 Version 1 (Component)
Coding sequence for LacI modified with a different rate of translation than LacI, RBS

RQE
BBa_I744104 Version 1 (Component)
tetracycline sensor with ptet promoter

06QE
BBa_I744103 Version 1 (Component)
Tetracycline sensor with strong promoter

13QE
BBa_I744101 Version 1 (Component)
Tetracycline sensor with weak promoter

BBa_J24671
BBa_J24671 Version 1 (Component)
Tri-Stable Toggle (Tetracycline induced component)

BBa_K362004
BBa_K362004 Version 1 (Component)
sodA promoter is controlled by some transcription factors including SoxS.

BBa_K1323022
BBa_K1323022 Version 1 (Component)
Ribosome binding site from the sodA Staphylococcus aureus gene

BBa_K1228004
BBa_K1228004 Version 1 (Component)
A fragment of loctoferrin

BBa_K1072019
BBa_K1072019 Version 1 (Component)
pGAL1 + Brho + Flag + odr-10 + GFP +ADH1 Te + pFUS1 + BFP + ADH1 Te

BBa_K362005
BBa_K362005 Version 1 (Component)
sufA , this promoter is controlled by OxyR.

TRM
BBa_K1145001 Version 1 (Component)
A transporter which is involved in transporting atrazine.