BBa_K085006
BBa_K085006 Version 1 (Component)
pTet->lock3d->GFP->Ter

BBa_K175022
BBa_K175022 Version 1 (Component)
PTet+Lock3c+GFP+TT

BBa_K1695000
BBa_K1695000 Version 1 (Component)
Tightly-regulated lactose-inducible promoter

BBa_E7103
BBa_E7103 Version 1 (Component)
Reporter Device Powered by the Virtual Machine (VM1.0)

BBa_K543032
BBa_K543032 Version 1 (Component)
assay system of killing by conjugation

BBa_K1395003
BBa_K1395003 Version 1 (Component)
sqr gene (sulfide quinone reductase) and cysI gene (sulfur reductase) under constitutive promoter

Kust2860
BBa_K1952001 Version 1 (Component)
Hydrazine Synthase beta subunit

BBa_K1952002
BBa_K1952002 Version 1 (Component)
Hydrazine Synthase alpha subunit

BBa_K103020
BBa_K103020 Version 1 (Component)
omega_linker under PT7

BBa_K103019
BBa_K103019 Version 1 (Component)
alpha_linker under PT7

BBa_K395160
BBa_K395160 Version 1 (Component)
RBS-CmR(no promoter and terminator)

BBa_K1947020
BBa_K1947020 Version 1 (Component)
This polypeptide will be fused with the recombinant protein which we want to purify.

BBa_J29051
BBa_J29051 Version 1 (Component)
Pinene synthase

BBa_K2034000
BBa_K2034000 Version 1 (Component)
Ocimene Synthase

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1956020
BBa_K1956020 Version 1 (Component)
RIBOSOFF with plasmid control

BBa_K1106000
BBa_K1106000 Version 1 (Component)
mRFP generator under hybrid promoter: HSL-LuxR activated, P22 C2 repressed

BBa_K1605013
BBa_K1605013 Version 1 (Component)
Ptet mRFP nifB RBS

BBa_K1605003
BBa_K1605003 Version 1 (Component)
Ptet mRFP nifZ RBS

BBa_K1605005
BBa_K1605005 Version 1 (Component)
Ptet mRFP nifE RBS

BBa_K1212013
BBa_K1212013 Version 1 (Component)
pTet + TBS1 + RBS + GFP

BBa_K1605015
BBa_K1605015 Version 1 (Component)
Ptet mRFP nifD RBS

BBa_K1605009
BBa_K1605009 Version 1 (Component)
Ptet mRFP nifS RBS

BBa_S04145
BBa_S04145 Version 1 (Component)
The Partial Butanol Operon (Parts I725021+I725022+I725023)

BBa_K177033
BBa_K177033 Version 1 (Component)
pcI lam RBS.3 LacI pcl lam RBS mRFP1 terminator

SspDnaB(N*
BBa_K1362142 Version 1 (Component)
RBS + SspDnaB N-intein RFC[105] non-splicing control construct (with His6)

BBa_K152006
BBa_K152006 Version 1 (Component)
frdBCD operon from E. coli (missing the first gene fumarate oxidase)

BBa_K880003
BBa_K880003 Version 1 (Component)
Asymmetrically digestible reporter, same orientation of inverted repeats as K137058 (external sites

BBa_K873003
BBa_K873003 Version 1 (Component)
conjugative transfer of RFG

BBa_K1952000
BBa_K1952000 Version 1 (Component)
Hydrazine Synthase gamma subunit

BBa_J10061
BBa_J10061 Version 1 (Component)
pTet+BD18+RFP_withoutATG no scars

BBa_S10000
BBa_S10000 Version 1 (Component)
CheY under plac

BBa_J202039
BBa_J202039 Version 1 (Component)
Ptet Characterization (Mutant#1)

BBa_J202041
BBa_J202041 Version 1 (Component)
Ptet Characterization (Mutant#3)

BBa_J202042
BBa_J202042 Version 1 (Component)
Ptet Characterization (Mutant#4)

BBa_J202043
BBa_J202043 Version 1 (Component)
Ptet Characterization (Mutant#5)

BBa_J202047
BBa_J202047 Version 1 (Component)
Ptet Characterization (Wild-type)

BBa_J202040
BBa_J202040 Version 1 (Component)
Ptet Characterization (Mutant#2)

BBa_J202045
BBa_J202045 Version 1 (Component)
Ptet Characterization (Mutant#7)

BBa_K204096
BBa_K204096 Version 1 (Component)
ptet+crtE+crtB+crtZ

BBa_J202046
BBa_J202046 Version 1 (Component)
Ptet Characterization (Mutant#8)

BBa_J202044
BBa_J202044 Version 1 (Component)
Ptet Characterization (Mutant#6)

BBa_K1825008
BBa_K1825008 Version 1 (Component)
Stilbene synthase

BBa_K103017
BBa_K103017 Version 1 (Component)
OmpA_linker_alpha_linker under Plac

BBa_K1854001
BBa_K1854001 Version 1 (Component)
GFP under QS

BBa_K103018
BBa_K103018 Version 1 (Component)
OmpA_linker_omega_linker under Plac

BBa_I12019
BBa_I12019 Version 1 (Component)
Test Construct of BBa_I12007 promoter

B-M
BBa_K774000 Version 1 (Component)
Bacterial-Mammalian Promoter: BBaK216005 + BHN + CArG promoter sequence E9-ns2

BBa_K1613009
BBa_K1613009 Version 1 (Component)
A composite part that can detect the recA.

BBa_K1613010
BBa_K1613010 Version 1 (Component)
A composite part that can detect the recA.