BBa_K145278
BBa_K145278 Version 1 (Component)
T7 DNA polymerase regulated by [lock3d]

LigA
BBa_K525720 Version 1 (Component)
DNA ligase from Escherichia coli (LigA)

BBa_K1520013
BBa_K1520013 Version 1 (Component)
Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter

BBa_K1316007
BBa_K1316007 Version 1 (Component)
yqjF promoter coupled to mKate2 reporter gene and Rhamnose promoter coupled to N-genes

BBa_K1316008
BBa_K1316008 Version 1 (Component)
ybiJ promoter coupled to mKate2 reporter gene and Rhamnose promoter coupled to N-genes

agaA
BBa_J26006 Version 1 (Component)
Beta Agarase A (raw DNA)

BBa_K1673202
BBa_K1673202 Version 1 (Component)
Formamidopyrimidine DNA glycosylase (Mutation Optimized)

BBa_K563054
BBa_K563054 Version 1 (Component)
Vector pYD, and can transfer into nucles of S.cerevisivae

BBa_K1159120
BBa_K1159120 Version 1 (Component)
Red light triggered TEV Protease with FRET Reporter for plants translation unit (PhyB/PIF6 version)

BBa_K1493560
BBa_K1493560 Version 1 (Component)
pRHA+cIλ+pcI/tet+GFP

BBa_I712059
BBa_I712059 Version 1 (Component)
CMVp - myri - TEVP - T7 RNAP - NLS

BBa_K1677801
BBa_K1677801 Version 1 (Component)
IPTG inducible promoter with TGV pseudoknot

BBa_K196008
BBa_K196008 Version 1 (Component)
Lux cassette + LacI promoter + cI434 + ter

BBa_B0022
BBa_B0022 Version 1 (Component)
TE from coliphageT7, reversed

BBa_I718020
BBa_I718020 Version 1 (Component)
ptet-lox71-rbs-gfp-ter-lox66-mRFP

BBa_K176129
BBa_K176129 Version 1 (Component)
TetR repressible AHL->PoPS Receiver (Low LuxR): pCon 0.04->luxR+pLux/Tet->PoPS

BBa_K1976050
BBa_K1976050 Version 1 (Component)
Colicin E2 DNase domain with B0034 RBS

BBa_I13712
BBa_I13712 Version 1 (Component)
CheR behind a tet promoter and Elowitz RBS

BBa_K165040
BBa_K165040 Version 1 (Component)
Gli1 binding sites + TEF constitutive yeast promoter

BBa_J70634
BBa_J70634 Version 1 (Component)
Upstream genomic DNA fragment of Mesoplasma florum for recombination experiment

BBa_K1179007
BBa_K1179007 Version 1 (Component)
(Acyl-TyA-TCS-rtTA3-His) A membrane/exosome targeting rtTA3 fusion with a TEV clevage site

T7+RBS+Ter
BBa_I716118 Version 1 (Component)
1106H RBS + T7rnap GTG + 1092 Term

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_I715002
BBa_I715002 Version 1 (Component)
pLac-lox-RBS-Tet (in pSB3K3)

BBa_K624002
BBa_K624002 Version 1 (Component)
pT7+RBS+tetR+RBS+GFP+Ter

BBa_M1207
BBa_M1207 Version 1 (Component)
Temperature Sensitive Hairpin Loop + Tet Repressor

BBa_K1997025
BBa_K1997025 Version 1 (Component)
P+R->sHRP-C->dCas9->Ter

BBa_K1040007
BBa_K1040007 Version 1 (Component)
toggle switch construction kit ( tet promoter_rbs_LacI_rbs )

BBa_K146001
BBa_K146001 Version 1 (Component)
AOX1 ethanol sensitive promoter cloned into RFP vector BBa_J61002

Prefix-R
BBa_G1003 Version 1 (Component)
Reverse primer for amplifying BioBrick plasmid backbones by PCR (Prefix-r)

BBa_K142020
BBa_K142020 Version 1 (Component)
tet-controlled lacI IS mutant (R197A, T276A) generator

BBa_K142021
BBa_K142021 Version 1 (Component)
tet-controlled lacI IS mutant (R197A, T276F) generator

BBa_K142022
BBa_K142022 Version 1 (Component)
tet-controlled lacI IS mutant (R197F, T276A) generator

BBa_K142023
BBa_K142023 Version 1 (Component)
tet-controlled lacI IS mutant (R197F, T276F) generator

BBa_K1159119
BBa_K1159119 Version 1 (Component)
Red light triggered TEV Protease with FRET Reporter for plants translation unit (PhyB/PIF3 version)

BBa_K273014
BBa_K273014 Version 1 (Component)
RBS - pdc - RBS - adh2(Y) - Ter

BBa_K327002
BBa_K327002 Version 1 (Component)
Polycistronic Delta, Tet, and 434 cI

BBa_K273013
BBa_K273013 Version 1 (Component)
RBS - pdc - RBS - adh2(Z) - Ter

BBa_I14056
BBa_I14056 Version 1 (Component)
Tet Toggle (strong RBS, weaker RBS)

BBa_I13306
BBa_I13306 Version 1 (Component)
R0040.I13279 Tet-Regulated Las Amplifier

BBa_I14030
BBa_I14030 Version 1 (Component)
Tet Toggle (strong RBS, weak RBS)

O2
BBa_K2054002 Version 1 (Component)
Oligo 2 of DNA Tetrahedral Nanostructure

O4
BBa_K2054004 Version 1 (Component)
Oligo 4 of DNA Tetrahedral Nanostructure

O3
BBa_K2054003 Version 1 (Component)
Oligo 3 of DNA Tetrahedral Nanostructure

O5
BBa_K2054005 Version 1 (Component)
Oligo 5 of DNA Tetrahedral Nanostructure

BBa_K103013
BBa_K103013 Version 1 (Component)
linker fused to omega fragment of TEM beta-lactamase

BBa_K103009
BBa_K103009 Version 1 (Component)
linker fused to alpha fragment of TEM beta-lactamase

Bxb1_Xis
BBa_K907001 Version 1 (Component)
Mycobacterium Phage Bxb1 gp47, DNA excisionase

IBMc325
IBMc325 Version 1 (Component)
pSB3T5(BsaI-)-PBAD(SapI-)-B30-mCherry*(1-192)-G-37R3-2-S-mCherry*(193-236)-H6-Ter

BBa_Z0271
BBa_Z0271 Version 1 (Component)
T7.2 TE transcriptional terminator