BBa_I3422BBa_I3422 Version 1 (Component)Test: Switch response to aspartate input (I3410.E0422.I3411.E0432.I3100.I3400.I3301.I3400.I3102.I340
BBa_K987001BBa_K987001 Version 1 (Component)This is a composite part which has the function to invert the temperature activation by the part: BB
BBa_K2044000BBa_K2044000 Version 1 (Component)Based on our project, <2-6-8> is the optimal pathway scheme from Site No. 2 to Site No. 8
BBa_M11085BBa_M11085 Version 1 (Component)E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o
CMV + GFPBBa_K1852000 Version 1 (Component)GFP + CMV Promoter used for expression in oocyte cells. This part was planned to be used as a report
BBa_K1334028BBa_K1334028 Version 1 (Component)IPTG activated promoter similar to R0011
BBa_K2044006BBa_K2044006 Version 1 (Component)Based on our project,<3,6> is the direct pathway from Site No.3 to Site No.6 in the map we design.
BBa_K2044004BBa_K2044004 Version 1 (Component)Based on our project, <2,4> is the direct pathway from Site No.2 to Site No.4 in the map we design.
BBa_K2044009BBa_K2044009 Version 1 (Component)Based on our project, <4,8> is the direct pathway from Site No.4 to Site No.8 in the map we design.
BBa_K2044014BBa_K2044014 Version 1 (Component)Based on our project, <1,4> is the direct pathway from Site No.1 to Site No.4 in the map we design.
BBa_K2044007BBa_K2044007 Version 1 (Component)Based on our project, <4,5> is the direct pathway from Site No.4 to Site No.5 in the map we design.
BBa_K2044013BBa_K2044013 Version 1 (Component)Based on our project,<7,8> is the direct pathway from Site No.7 to Site No.8 in the map we design.
BBa_K2044008BBa_K2044008 Version 1 (Component)Based on our project, <4,6> is the direct pathway from Site No.4 to Site No.6 in the map we design.
BBa_K2044003BBa_K2044003 Version 1 (Component)Based on our project, <2,3> is the direct pathway from Site No.2 to Site No.3 in the map we design.
BBa_K2044002BBa_K2044002 Version 1 (Component)Based on our project, <2,1> is the direct pathway from Site No.2 to Site No.1 in the map we design.
BBa_K2044005BBa_K2044005 Version 1 (Component)Based on our project, <2,6> is the direct pathway from Site No.2 to Site No.6 in the map we design.
BBa_K2044012BBa_K2044012 Version 1 (Component)Based on our project, <6,8> is the direct pathway from Site No.6 to Site No.8 in the map we design.
BBa_K2044011BBa_K2044011 Version 1 (Component)Based on our project,<6,4> is the direct pathway from Site No.6 to Site No.4 in the map we design.
VVD-YCBBa_K1616021 Version 1 (Component)VVD linked to YC155 (YFP Cter split)
BBa_K1616004BBa_K1616004 Version 1 (Component)VVD linked to YN155 (YFP Nter split)
BBa_J04795BBa_J04795 Version 1 (Component)Riboswitch designed to turn "ON" a protein
BBa_K1321362BBa_K1321362 Version 1 (Component)sfGFP fused to CBDcex driven by LacI
BBa_K936012BBa_K936012 Version 1 (Component)Leader sequence that brings protein to periplasm
BBa_I733004BBa_I733004 Version 1 (Component)Produce LacZ alpha in response to AHL
BBa_K1615108BBa_K1615108 Version 1 (Component)mRFP fused to CBDclos driven by LacI promoter
BBa_I758600BBa_I758600 Version 1 (Component)Screen for binding affinity of mutant cI lambda to promotor sites
BBa_K1657006BBa_K1657006 Version 1 (Component)It is called GAB. It have the resistance to glyphosate and glufosinate
[OriTR]+[RBBa_K1439002 Version 1 (Component)This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.
BBa_K1113411BBa_K1113411 Version 1 (Component)Targeting sequence for the delivery of the LacZ gene to the Carboxysome
BBa_K1412088BBa_K1412088 Version 1 (Component)A combination of theophylline aptamer and taRNA that can response theophylline to regulate circuit
BBa_K541715BBa_K541715 Version 1 (Component)Multi-host vector pTG262 converted to BioBrick vector wtih LALF protein and SacB signal peptide
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
SBOLDesigner CAD ToolSBOLDesigner Version 3.0 (Agent)SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.