| Types | DnaRegion
|
| Roles | engineered_region
Project
|
| Sequences | BBa_I3422_sequence (Version 1)
|
Description
Tests ability of switch to respond to aspartate input by connecting the Tar-EnvZ receptor (I3301 truncated ompC upstream version) to one of the switch inputs. The remaining testbed is analogous to the switch functionality testbed (I3420).
Notes
The layout of the switch mimics the layout of the proposed Polkadorks E. colibrator (I3000). Two switch outputs are insulated by appearing first. Hk022cI is attached to the CFP reporter (I3410.E0422). Lambda cI is attached to the YFP reporter (I3411.E0432). The three individually controlled trigger inputs follow the outputs in the layout. Lac trigger controls one hk022cI input (I3100.I3400), and Tar-EnvZ receptor (I3301 truncated upstream ompC version) controls the other (I3301.I3400). The dual control forms the built-in OR gate in one of the switch inputs. Finally, AraC trigger controls the lambda cI input (I3102.I3401).
