BBa_K1819008BBa_K1819008 Version 1 (Component)Promoter test circuit to analyze the kill switch efficiency
BBa_K654030BBa_K654030 Version 1 (Component)Anderson 1.0 Promoter - Promoter only part
BBa_K875007BBa_K875007 Version 1 (Component)T5 LacO-PelB-SIP (scFv 54.6 + alpha isotype CH3 antinorovirus)-6HIS-TT
BBa_K875005BBa_K875005 Version 1 (Component)T5LacO-LPP-OmpA-SIP (scFv 54.6 + alpha isotype CH3 antinorovirus)-6HIS-TT
BBa_K2117011BBa_K2117011 Version 1 (Component)ECFP under regulation of the Y. lipolytica native promoter TEF1
BBa_K199070BBa_K199070 Version 1 (Component)I13453:K199016 Pbad promotor with the suppressor tRNA of CUAGC
BBa_K1440002BBa_K1440002 Version 1 (Component)part1 or our whole design,SV40>PTRE>EYFP>loxP>Ribozyme>SV40
BBa_K1058003BBa_K1058003 Version 1 (Component)Promoter of penicillinase blaZ as well as promoter of blaR1
BBa_J70596BBa_J70596 Version 1 (Component)J70577 cut with BsaXI and with 6his inserts (J70564-fi) added
BBa_K1162005BBa_K1162005 Version 1 (Component)Scygonadin antimicrobial peptide from the horseshoe crab (<i>Scylla serrata</i>)
BBa_K1893001BBa_K1893001 Version 1 (Component)Las receiver with GFP reporter (LasR+pLas+GFP)
BBa_I13211BBa_I13211 Version 1 (Component)Biobricked version of the natural Lux quorum sensing system
BBa_K748007BBa_K748007 Version 1 (Component)Biofilm formation device. yddV gene with the IPTG inducible promoter.
BBa_K188006BBa_K188006 Version 1 (Component)Expression of ccdB for self-destruction of bacteria. Since promoter lux/cIIp22, this sequence can be
BBa_M1678BBa_M1678 Version 1 (Component)a construct to detect the presence/absence of glucose
BBa_K112210BBa_K112210 Version 1 (Component){< int >} The integrase gene without start and stop codons, BBb format
BBa_K112214BBa_K112214 Version 1 (Component){< ihfA!} The ihf alpha gene without a start codon, BBb format
BBa_K112215BBa_K112215 Version 1 (Component){ihfA>} The ihf alpha gene without a stop codon, BBb format
BBa_K112220BBa_K112220 Version 1 (Component){< ihfB!} The ihf beta gene without a start codon, BBb format
BBa_K112221BBa_K112221 Version 1 (Component){ihfB>} The ihf beta gene without a stop codon, BBb format
BBa_K112207BBa_K112207 Version 1 (Component){int!} The integrase gene with start and stop codon, BBb format
BBa_K133121BBa_K133121 Version 1 (Component)CMV-HA-UreB
Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_K327004BBa_K327004 Version 1 (Component)pBad Promoter+ RBS + Las Membrane Protein + double terminator
BBa_K1334020BBa_K1334020 Version 1 (Component)a signal complex to test whether the lux system works
BBa_I715076BBa_I715076 Version 1 (Component)pLac promoter upstream of the Ec86 retron msr/msd sequence
BBa_I715075BBa_I715075 Version 1 (Component)pLac promoter upstream of the Ec83 retron msr/msd sequence
BBa_K2100038BBa_K2100038 Version 1 (Component)pEXPR TRE - TALER21
BBa_K2100069BBa_K2100069 Version 1 (Component)pEXPR TRE: L7Ae
BBa_K2100075BBa_K2100075 Version 1 (Component)pEXPR TRE: mKate
BBa_K2100079BBa_K2100079 Version 1 (Component)pEXPR TRE: tagBFP
BBa_I733002BBa_I733002 Version 1 (Component)big toe meter
BBa_K2100037BBa_K2100037 Version 1 (Component)pEXPR TRE - TALER14
BBa_K2100036BBa_K2100036 Version 1 (Component)pEXPR TRE-BM3R1
BBa_K1911006BBa_K1911006 Version 1 (Component)pLamdaR-LacI-GFP-DAS-pLac-ClpXP-CI
BBa_K1781004BBa_K1781004 Version 1 (Component)T18 - one of two fragments of the T25/T18 BACTH system
BBa_M45689BBa_M45689 Version 1 (Component)Constitutive promoter with a retinoic acid response element at the end
BBa_K363003BBa_K363003 Version 1 (Component)A calcium dependent response element binding site for the Crz1 activator
BBa_K363005BBa_K363005 Version 1 (Component)A calcium dependent response element binding site for the Crz1 activator
BBa_K363007BBa_K363007 Version 1 (Component)A calcium dependent response element binding site for the Crz1 activator
BBa_K363004BBa_K363004 Version 1 (Component)A calcium dependent response element binding site for the Crz1 activator
BBa_K112229BBa_K112229 Version 1 (Component){a~pelB} The pelB leader ready to attach rbs, BBb format
BBa_K1465227BBa_K1465227 Version 1 (Component)sRNA of phosphofructokinase A under the control of T7 promoter (T7_sRNA:pfkA)
BBa_K112226BBa_K112226 Version 1 (Component){a~ompT} The ompT gene ready to attach rbs, BBb site
BBa_K1465226BBa_K1465226 Version 1 (Component)sRNA of phosphofructokinase A under the control of ptac promoter (ptac_sRNA:pfkA)
BBa_K363012BBa_K363012 Version 1 (Component)Cnb1, the calcium sensitive regulatory subunit for calcineurin from S. cerevisiae
BBa_K1781003BBa_K1781003 Version 1 (Component)T25 - one of two fragments of the T25/T18 BACTH system
BBa_K935005BBa_K935005 Version 1 (Component)Two E. coli ArsR generators coupled to pArs-ArsR-RFP
BBa_K1778005BBa_K1778005 Version 1 (Component)eGFP:enhanced Green Fluorescent Protein. It???s the mutant of GFP. It is widely used as report gene