Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jackson Harris
Date created: 2016-10-20 11:00:00
Date modified: 2016-10-21 01:26:48
pLamdaR-LacI-GFP-DAS-pLac-ClpXP-CI
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1911006_sequence (Version 1) |
Description
This construct is an example of the switch system that was used in our project. Once the E. coli cells containing our construct was induced with IPTG, the pLamdaR pathway would be shut down while the pLac pathway would be turned on. This allows for the degradation of the gene of interest in which this case is the eGFP. This construct is the finished product of our project after using 3A Assembly to assemble the two different promoter pathways. This construct is switchable because IPTG can be added to switch the system from one pathway to another. NOTE: There is no Biobrick scar between Bba_K1911005 and Bba_M0050. The Sequence given will contain a scar of, "tactagag" which is to be ignored.Notes
NoSource
Individual Pieces come from genomic sequences. The overall part is a composite created via AAA Assembly| Access | Instance | Definition |
| public public public public public public public public public public public public public public public | BBa_R0051 BBa_B0033 BBa_C0012 BBa_B0034 BBa_K1911005 BBa_M0051 BBa_B0010 BBa_B0012 BBa_R0011 BBa_B0033 BBa_K1911003 BBa_B0033 BBa_K1911002 BBa_B0033 BBa_C0051 | cI lam BBa_B0033 lacI BBa_B0034 BBa_K1911005 BBa_M0051 BBa_B0010 BBa_B0012 lacI+pL BBa_B0033 BBa_K1911003 BBa_B0033 BBa_K1911002 BBa_B0033 cI lam |