BBa_K105008
BBa_K105008 Version 1 (Component)
EYFP, yeast optimized for fusion proteins

BBa_K1114003
BBa_K1114003 Version 1 (Component)
The MoClo format of BBa_J23103 with AB fusion sites.

cln2
BBa_K105014 Version 1 (Component)
cln2 PEST destabilization domain for rapid protein turnover

BBa_K351004
BBa_K351004 Version 1 (Component)
Modified Human FGF receptor for MPT51 antigen with fission yeast surface signal peptide

BBa_K1051355
BBa_K1051355 Version 1 (Component)
K1051300 (cln2 promoter) + K1051053(K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 ter

BBa_K1051357
BBa_K1051357 Version 1 (Component)
K1051302 (clb5 promoter) + K1051053(K1051001 (non stop codon ECFP + K1051006 (Stop codon + TBY-1 ter

BBa_I20293
BBa_I20293 Version 1 (Component)
Constitutive expression of GFP-LacZalpha fusion

BBa_K1520509
BBa_K1520509 Version 1 (Component)
PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter

BBa_K1413045
BBa_K1413045 Version 1 (Component)
A fusion of Universal Transposon Plasmid and pSB1C3

BBa_K1088057
BBa_K1088057 Version 1 (Component)
T25 domain of bacterial two-hybrid system (IPTG inducible)

BBa_K748000
BBa_K748000 Version 1 (Component)
AgrA protein coding sequence. AgrA is the main component of S.aureus agr quorum sensing system.

BBa_K748001
BBa_K748001 Version 1 (Component)
AgrC protein coding sequence. AgrC is main component of S.aureus agr quorum sensing system.

AraC_TEV-F
BBa_K627008 Version 1 (Component)
Fusion part of arabinose-inducible induction system and the TEV protease

BBa_K300096
BBa_K300096 Version 1 (Component)
Double phasin and intein separed by a flexible protein domain linker

ssTorA_CS-
BBa_K627012 Version 1 (Component)
Fusion of TorA sig-seq, TEV protease cleavage site and b-lactamase

BBa_K371054
BBa_K371054 Version 1 (Component)
MPF(meta-prefix)+[GFP+10*GS+A] fusion protein+MSF(meta-suffix))

BBa_K774102
BBa_K774102 Version 1 (Component)
Multi sensor - for calculation of specific concentrations of nitrates nitrites and nitric oxide usin

BBa_K196013
BBa_K196013 Version 1 (Component)
Hybrid promoter (Lux cassette + c2 P22 promoter) + RBS + LuxR + ter

BBa_K351000
BBa_K351000 Version 1 (Component)
Signal peptide for cell surface of fission yeast

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.