SspGyrB(N)
BBa_K1362120 Version 1 (Component)
RBS + SspGyrB N-intein RFC[105] assembly construct (with His6)

SspGyrB(C)
BBa_K1362121 Version 1 (Component)
RBS + SspGyrB C-intein RFC[105] assembly construct (with His6)

SspDnaE(C)
BBa_K1362411 Version 1 (Component)
SspDnaE C-Intein cloning piece

BBa_K195200
BBa_K195200 Version 1 (Component)
Human alpha-V integrin gene

1NpuE(C*)
BBa_K1362103 Version 1 (Component)
RBS + NpuDnaE C-intein RFC[105] non-slicing control construct (with His6)

BBa_K1362111
BBa_K1362111 Version 1 (Component)
RBS + SspDnaX C-intein RFC[105] assembly construct (with His6)

BBa_K300084
BBa_K300084 Version 1 (Component)
Phasins with intein downstream (separated by a flexible protein domain linker)

Gp41-1(C)
BBa_K1362413 Version 1 (Component)
Gp41-1 C-Intein cloning piece

BBa_K1707029
BBa_K1707029 Version 1 (Component)
thermosensitive cI repressor controlling the expression of CFP-ssRA

BBa_K1491025
BBa_K1491025 Version 1 (Component)
T7 RNA Polymerase with ER Intein

BBa_K1362444
BBa_K1362444 Version 1 (Component)
GGG linker with RGK (extein)

BBa_K300095
BBa_K300095 Version 1 (Component)
Phasin and intein separated by a flexible protein domain linker

BBa_K1916011
BBa_K1916011 Version 1 (Component)
CBCR2 + intein-CBD tag

BBa_K1916022
BBa_K1916022 Version 1 (Component)
CBCR11 + intein-CBD tag

BBa_K1916005
BBa_K1916005 Version 1 (Component)
Ga0100955_11928g3 + intein-CBD tag

BBa_K1916008
BBa_K1916008 Version 1 (Component)
Osc7112_5903g3 + intein-CBD tag

BBa_K1916010
BBa_K1916010 Version 1 (Component)
CBCR2 + intein-CBD tag

BBa_K1916023
BBa_K1916023 Version 1 (Component)
CBCR11 + intein-CBD tag

BBa_K1916017
BBa_K1916017 Version 1 (Component)
CBCR6 + intein-CBD tag

BBa_K1916015
BBa_K1916015 Version 1 (Component)
CBCR4 + intein-CBD tag

BBa_K1916020
BBa_K1916020 Version 1 (Component)
CBCR9 + intein-CBD tag

BBa_K1916019
BBa_K1916019 Version 1 (Component)
CBCR8 + intein-CBD tag

BBa_K1916009
BBa_K1916009 Version 1 (Component)
Osc7112_5903g3 + intein-CBD tag

BBa_K1916001
BBa_K1916001 Version 1 (Component)
NpF2164g5 + intein-CBD tag

BBa_K1916018
BBa_K1916018 Version 1 (Component)
CBCR8 + intein-CBD tag

BBa_K1916013
BBa_K1916013 Version 1 (Component)
CBCR3 + intein-CBD tag

BBa_K1916007
BBa_K1916007 Version 1 (Component)
Ga0039498_104087g2 + intein-CBD tag

BBa_K1916014
BBa_K1916014 Version 1 (Component)
CBCR4 + intein-CBD tag

BBa_K1916000
BBa_K1916000 Version 1 (Component)
NpF2164g5 + intein-CBD tag

BBa_K1916003
BBa_K1916003 Version 1 (Component)
Cha6605_4344g2 + intein-CBD tag

BBa_K1916006
BBa_K1916006 Version 1 (Component)
Ga0039498_104087g2 + intein-CBD tag

BBa_K1435000
BBa_K1435000 Version 1 (Component)
4-HT inducible intein

BBa_K1916002
BBa_K1916002 Version 1 (Component)
Cyan7427_1390g3 + intein-CBD tag

BBa_K1916021
BBa_K1916021 Version 1 (Component)
CBCR9 + intein-CBD tag

BBa_K1916004
BBa_K1916004 Version 1 (Component)
Ga0100955_11928g3 + intein-CBD tag

BBa_K1916016
BBa_K1916016 Version 1 (Component)
CBCR6 + intein-CBD tag

BBa_K1916012
BBa_K1916012 Version 1 (Component)
CBCR3 + intein-CBD tag

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1362160
BBa_K1362160 Version 1 (Component)
RBS + Gp41-1 N-intein RFC[105] assembly construct (with His6)

A
BBa_G0009 Version 1 (Component)
The nucleotide A

RGK
BBa_K1362434 Version 1 (Component)
RGK (extein)

BBa_K1679022
BBa_K1679022 Version 1 (Component)
KIG, kcoil+intein+gfp

3xSWAG(N)
BBa_K1362900 Version 1 (Component)
N-terminal tripleSWAGtag (N-intein)

BBa_K1362901
BBa_K1362901 Version 1 (Component)
C-terminal tripleSWAGtag (C-intein)

BBa_K300083
BBa_K300083 Version 1 (Component)
Phasins (separated by a flexible protein domain linker) with intein downstream

BBa_K1491027
BBa_K1491027 Version 1 (Component)
T7 RNA Polymerase with Dead ER Intein

SspDnaB(N*
BBa_K1362142 Version 1 (Component)
RBS + SspDnaB N-intein RFC[105] non-splicing control construct (with His6)

BBa_K1638035
BBa_K1638035 Version 1 (Component)
hTrx-based scaffold fused to T18 through intein and a flexible linker

BBa_K1051704
BBa_K1051704 Version 1 (Component)
Mer2 Intron