BBa_K1114006
BBa_K1114006 Version 1 (Component)
The MoClo format of BBa_J23104 with EB fusion sites.

BBa_K1510624
BBa_K1510624 Version 1 (Component)
Truncated lysostaphin coding sequence plus strong promoter and yebf

AraC_TEV-F
BBa_K627008 Version 1 (Component)
Fusion part of arabinose-inducible induction system and the TEV protease

AraC_TEV-F
BBa_K627010 Version 1 (Component)
Fusion of AraC induction system and TEV protease 3

BBa_K783056
BBa_K783056 Version 1 (Component)
Level 0 MoClo Destination Vector with EB

BBa_K315035
BBa_K315035 Version 1 (Component)
pTet-loxBri(F)-RBS-RFP-lox2272

BBa_K315025
BBa_K315025 Version 1 (Component)
pTet-loxBri(F)-RBS-RFP-loxN

BBa_K315018
BBa_K315018 Version 1 (Component)
pTet-loxP-RBS-RFP-loxBri(F)

BBa_K1114019
BBa_K1114019 Version 1 (Component)
The MoClo format of BBa_J23116 with EB fusion sites.

BBa_K1114026
BBa_K1114026 Version 1 (Component)
The MoClo format of BBa_R0040 with EB fusion sites.

BBa_K1114030
BBa_K1114030 Version 1 (Component)
The MoClo format of BBa_R0063 with EB fusion sites.

BBa_K1114017
BBa_K1114017 Version 1 (Component)
The MoClo format of BBa_J23115 with EB fusion sites.

BBa_K1114015
BBa_K1114015 Version 1 (Component)
The MoClo format of BBa_J23112 with EB fusion sites.

BBa_K1114008
BBa_K1114008 Version 1 (Component)
The MoClo format of BBa_J23105 with EB fusion sites.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.