BBa_K584025
BBa_K584025 Version 1 (Component)
Constitutive Promotor (J23119) + GFP generator

BBa_K1157007
BBa_K1157007 Version 1 (Component)
Quorum sensing receptor pqsR generator

BBa_K831014
BBa_K831014 Version 1 (Component)
Inducible istR (inhibitor of SOS-induced toxicity by RNA) under the control of lac promoter

BBa_J06201
BBa_J06201 Version 1 (Component)
Tet repressible rhI generator (LVA+)

BBa_J06101
BBa_J06101 Version 1 (Component)
Tet repressible lasI generator ( LVA+ )

BBa_K235010
BBa_K235010 Version 1 (Component)
[K145303] (ribokey-controlled GFP generator)

BBa_K127002
BBa_K127002 Version 1 (Component)
constitutive GFP generator with J23102

BBa_K1189030
BBa_K1189030 Version 1 (Component)
TALE-B with a his6 tag linked to a K coil under an inducible lacI promoter

BBa_K1106013
BBa_K1106013 Version 1 (Component)
pag activator from PSP3 phage generator

BBa_E1040
BBa_E1040 Version 1 (Component)
RiPS-based Protein Generator: E0040.B0015

BBa_I754001
BBa_I754001 Version 1 (Component)
ech protein generator for vanillin biosynthesis

BBa_I769106
BBa_I769106 Version 1 (Component)
ech protein generator for vanillin biosynthesis

BBa_K175049
BBa_K175049 Version 1 (Component)
GFP generator with "normal" RBS (B0034)

BBa_K206005
BBa_K206005 Version 1 (Component)
GFP generator for testing pBAD strong

BBa_K175035
BBa_K175035 Version 1 (Component)
Constitutive expression of GFP with medium RBS lock and inducible production of key for the lock

BBa_K175034
BBa_K175034 Version 1 (Component)
Constitutive expression of GFP with weak RBS lock and inducible production of key for the lock

BBa_K091119
BBa_K091119 Version 1 (Component)
LacI protein generator with a pTet promoter

BBa_I534160
BBa_I534160 Version 1 (Component)
aiiA (LVA-) protein generator driven by pBad

BBa_I13035
BBa_I13035 Version 1 (Component)
3OC₆HSL Receiver Device with Inducible Control of LuxR and a YFP Output device

IPTG induc
BBa_K653003 Version 1 (Component)
IPTG inducible Expression Platform

BBa_K1403001
BBa_K1403001 Version 1 (Component)
GFP generator in pSB1C3 under a weak promoter

BBa_K1132014
BBa_K1132014 Version 1 (Component)
Promoter (OmpR, positive) followed by RFP protein generator

BBa_K584023
BBa_K584023 Version 1 (Component)
Ice Neucleating Protein Generator (RBS + InaZ [K584021] + Terminator)

BBa_I769103
BBa_I769103 Version 1 (Component)
Feruloyl-CoA hydratase for vanillin biosynthesis protein generator

BBa_K584022
BBa_K584022 Version 1 (Component)
Ice Neucleating Protein Generator (RBS + InaZ [K584021] + Terminator)

BBa_K204040
BBa_K204040 Version 1 (Component)
C₄HSL generater

BBa_K511905
BBa_K511905 Version 1 (Component)
Repressible rtTA3 Transactivator Generator (Hef1a-LacO-rtTA3) MammoBlock Device

BBa_K944001
BBa_K944001 Version 1 (Component)
Transcriptional Regulator for Cyanide Inducible Promoter

BBa_K204022
BBa_K204022 Version 1 (Component)
3O₆HSL generater device

BBa_K1954007
BBa_K1954007 Version 1 (Component)
Green Light Inducible bacteriocin Device (GLID)

BBa_I718001
BBa_I718001 Version 1 (Component)
ech My new test generator part Feruloyl CoA hydratase for vanilin

BBa_K1323019
BBa_K1323019 Version 1 (Component)
Hfq expression cassette under a xylose inducible promoter

BBa_K2092002
BBa_K2092002 Version 1 (Component)
PalcA, improved alcR inducible promoter from A. nidulans

BBa_K590046
BBa_K590046 Version 1 (Component)
AAR-PSB3K3-Lac Inducible w/o LacI

BBa_K418001
BBa_K418001 Version 1 (Component)
From partsregistry.org IPTG inducible Lac promoter cassette

BBa_K1407004
BBa_K1407004 Version 1 (Component)
LasR/PAI1 Inducible promoter +RBS(B0034)+GFP+Terminator

BBa_R4030
BBa_R4030 Version 1 (Component)
PoPS/RiPS Generator composed of the Tet promoter and a strong RBS (R0040.E0030)

Prom/RBS
BBa_K262000 Version 1 (Component)
BBa_R0011 & BBa_B0034, IPTG-inducible promoter with Elowitz RBS.

BBa_K831006
BBa_K831006 Version 1 (Component)
Inducible MqsR toxin under the control of tetR promoter

BBa_K1088057
BBa_K1088057 Version 1 (Component)
T25 domain of bacterial two-hybrid system (IPTG inducible)

BBa_K1968009
BBa_K1968009 Version 1 (Component)
PglaA inducible promoter Phytobrick: glucoamylase gene promoter (PglaA) from Aspergillus niger

BBa_K563053
BBa_K563053 Version 1 (Component)
vector pYE, designed for inducible expression of recombinant proteins in S.cerevisivae.

pSBBs0K
BBa_K823026 Version 1 (Component)
pSB_Bs0K-P_spac (replicative Bacillus subtilis expression vector; IPTG inducible

Adapter Bi
BBa_K1807015 Version 1 (Component)
This device allows for the IPTG-inducible expression of lacZα peptide which in the presence of

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.