BBa_K209432
BBa_K209432 Version 1 (Component)
pCEP4 with AarI A-D sites in MCS

BBa_K255000
BBa_K255000 Version 1 (Component)
laci-cfp fusion

RBS-rev
BBa_K1616011 Version 1 (Component)
RBS reversed

MaoA
BBa_K1615022 Version 1 (Component)
Monoamine Oxidase A RFC25

OmpA
BBa_K103006 Version 1 (Component)
OmpA outer membrane protein A fused to linker; displays proteins on cell surface

HerE
BBa_K1615045 Version 1 (Component)
Heroin Esterase in RFC25

BBa_K117000
BBa_K117000 Version 1 (Component)
Lysis gene (promotes lysis in colicin-producing bacteria strain)

BBa_K678016
BBa_K678016 Version 1 (Component)
eCFP-TS

eCFP-GOI
BBa_K678048 Version 1 (Component)
eCFP-GOI

eGFP
BBa_K2075001 Version 1 (Component)
enhanced GFP

BBa_I13600
BBa_I13600 Version 1 (Component)
Tet with CFP reporter (without LVA tag)

BBa_K315010
BBa_K315010 Version 1 (Component)
lox2272 Reverse

BBa_K1634002
BBa_K1634002 Version 1 (Component)
pmyo2 ( a promoter in C.elegans )

BBa_K1051004
BBa_K1051004 Version 1 (Component)
Stop codon free mOrange in RFC[23] standard

EYFP
BBa_K1319000 Version 1 (Component)
RFC[25] version of E0030

MB-CFP
BBa_K774006 Version 1 (Component)
Mammalian-Bacterial promoter with eCFP reporter: CArG promoter sequence + BBaK216005 + BBa_E0420

BBa_K2120106
BBa_K2120106 Version 1 (Component)
reverse tetR

BBa_K1440066
BBa_K1440066 Version 1 (Component)
reverse CMV->reverse RFP->pTRE->shRNA

BBa_K1641205
BBa_K1641205 Version 1 (Component)
FRT(reverse)-pBAD(reverse)

BBa_K2120100
BBa_K2120100 Version 1 (Component)
reverse arac-pBAD

BBa_K331024
BBa_K331024 Version 1 (Component)
RBS with N-terminal Oligo Arginine - ECFP Fusion

BBa_K1509001
BBa_K1509001 Version 1 (Component)
A bi-directional promoter affected by SmtB protein

BBa_K733001
BBa_K733001 Version 1 (Component)
<i>Ptms</i> - a constitutive promoter in <i>E. coli</i> and <i>B. subtilis</i>.

BBa_K313013
BBa_K313013 Version 1 (Component)
loading sequence of RNA phage MS2 (reverse)

BBa_J18960
BBa_J18960 Version 1 (Component)
eCFP

eGFP
BBa_K678006 Version 1 (Component)
eGFP for mammalian cells

BBa_K331025
BBa_K331025 Version 1 (Component)
RBS with C-terminal Oligo Arginine - ECFP Fusion

BBa_K407010
BBa_K407010 Version 1 (Component)
RBS + ecfp

BBa_I746104
BBa_I746104 Version 1 (Component)
P2 promoter in agr operon from S. aureus

CFP
BBa_K411201 Version 1 (Component)
Cyan Fluorescent Protein (CFP)

BBa_K1641209
BBa_K1641209 Version 1 (Component)
flpe-ECFP

BBa_K309012
BBa_K309012 Version 1 (Component)
Unc-54 3' UTR (for use in C. elegans chassis)

TVEL5
BBa_K1615067 Version 1 (Component)
Laccase TVEL5 in RFC25

recAp-cheZ
BBa_K629010 Version 1 (Component)
recAp-cheZ-eGFP

eYFP
BBa_K678009 Version 1 (Component)
eYFP-module

BBa_K309016
BBa_K309016 Version 1 (Component)
eGFP (for use in C. elegans chassis)

BBa_K1418041
BBa_K1418041 Version 1 (Component)
CFP(ns)

BBa_I13602
BBa_I13602 Version 1 (Component)
Tet operator with CFP reporter (with LVA tag) [R/Tc+]

BBa_K1758377
BBa_K1758377 Version 1 (Component)
Biosensor device for detection of GHB and GBL

BBa_K093008
BBa_K093008 Version 1 (Component)
reverse BBa_R0011

scar
BBa_G0001 Version 1 (Component)
XbaI/SpeI mixed site, reverse

R5
BBa_K1442103 Version 1 (Component)
Reverse 5

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K322128
BBa_K322128 Version 1 (Component)
Phycocyanobilin synthesis genes + Red light detection system (EYFP as reporter)

Pr+CFP
BBa_S03464 Version 1 (Component)
Pr with CFP as a reporter

BBa_I13601
BBa_I13601 Version 1 (Component)
Lac operator with CFP reporter (without LVA tag) [R/Lc-]

BBa_K1634005
BBa_K1634005 Version 1 (Component)
pmyo3 (a promoter in C.elegans)

BBa_J70029
BBa_J70029 Version 1 (Component)
RBS + mCherry gene, optimized for expression in Me. florum and E. coli

BBa_K1087009
BBa_K1087009 Version 1 (Component)
Ptet-reverse lox-reverse PO promoter

BBa_M45905
BBa_M45905 Version 1 (Component)
Composite part for fluorescent detection of botulinum neurotoxin A (BoNT A)