BBa_K783040
BBa_K783040 Version 1 (Component)
This is a MoClo converted version of BBa_J23110

BBa_K783034
BBa_K783034 Version 1 (Component)
This is a MoClo converted version of BBa_J23114

BBa_K346025
BBa_K346025 Version 1 (Component)
PmerT promoter mutant 88+RBS(B0030)+GFP(E0040)Terminator(B0010)+Terminator(B0012)

BBa_J47053
BBa_J47053 Version 1 (Component)
Constitutive device (medium transcription) for lacI repressor, strong RBS

BBa_K1675026
BBa_K1675026 Version 1 (Component)
P-atp2-B0034-lacz alpha

BBa_K200029
BBa_K200029 Version 1 (Component)
pLacI+RBS+RcsB+TT

BBa_K1084113
BBa_K1084113 Version 1 (Component)
SD6 LacZα dT

BBa_K228821
BBa_K228821 Version 1 (Component)
lacP(R0010)+GFP(E0840)

BBa_K876035
BBa_K876035 Version 1 (Component)
pLuxR-LasI-RBS-RFP

BBa_K896986
BBa_K896986 Version 1 (Component)
this is a gene about a T cell receptor

BBa_K136033
BBa_K136033 Version 1 (Component)
RBS lasI - gfp tripart

BBa_K1767005
BBa_K1767005 Version 1 (Component)
P(Lac)IQ RBS LuxR ter ter luxpR RBS tetR ter ter P(tetR) RBS mRFP1 ter ter

BBa_K136034
BBa_K136034 Version 1 (Component)
RBS lasI - gfp tripart

BBa_K132016
BBa_K132016 Version 1 (Component)
luxI+KanR-LVA+LacI+PL+KanR-LVA+aiiA+terminator

BBa_K346083
BBa_K346083 Version 1 (Component)
RBS(B0034)+lacZ alpha(BBa_I732006)

BBa_I756010
BBa_I756010 Version 1 (Component)
SV40 Introng with LacO Sites

BBa_K136056
BBa_K136056 Version 1 (Component)
RBS - lasI - ECFP Tripart (+LVA)

pCMV-ECFP-
BBa_I763023 Version 1 (Component)
LacI coding device with ECFP as a reporter regulated by pCMV

FBS-AceB+L
BBa_K1163999 Version 1 (Component)
Inverter composed of Fur Binding site from AceB promoter + LacI-LVA

BBa_K658021
BBa_K658021 Version 1 (Component)
GFP driven by lacl+pL

BBa_K294205
BBa_K294205 Version 1 (Component)
This is a coding sequence of heat shock protein from E.coli

BBa_K794001
BBa_K794001 Version 1 (Component)
CSP(derived from E. coli) - lasI

BBa_K1767003
BBa_K1767003 Version 1 (Component)
P(Lac)IQ RBS Aiia RBS LuxR ter ter luxpR RBS tetR ter ter P(tetR) RBS mRFP1 ter ter

BBa_K086000
BBa_K086000 Version 1 (Component)
unmodified Lutz-Bujard LacO promoter - YFP

BBa_K1657006
BBa_K1657006 Version 1 (Component)
It is called GAB. It have the resistance to glyphosate and glufosinate

BBa_K329005
BBa_K329005 Version 1 (Component)
Strong RBS (B0034) - LacZ-alpha fragment (I732006)

BBa_I733004
BBa_I733004 Version 1 (Component)
Produce LacZ alpha in response to AHL

BBa_I732091
BBa_I732091 Version 1 (Component)
Double Repoters (LacZ-alpha and GFP-AAV)

BBa_K564016
BBa_K564016 Version 1 (Component)
Upstream mutated chitoporin part fused with lacZ

BBa_K564017
BBa_K564017 Version 1 (Component)
Upstream mutated chitoporin part fused with lacZ

BBa_K737001
BBa_K737001 Version 1 (Component)
We got this part from the mutant of E.coli strain K12, DH5α,using PCR with the primers we desig

BBa_I715071
BBa_I715071 Version 1 (Component)
Lac promoter with Ribosomal Binding Site

BBa_K079021
BBa_K079021 Version 1 (Component)
LacI repressor and GFP reporter proteins under the control of the J23118 costitutive promoter and La

BBa_K1952012
BBa_K1952012 Version 1 (Component)
Hydrazine Synthase subunit alpha (Kust-2861) with LacZ reporter

BBa_K2150010
BBa_K2150010 Version 1 (Component)
This part consists of a gene encoding toxin 134 with a LacI gene, a Ptac promoter included

BBa_K831011
BBa_K831011 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_K831012
BBa_K831012 Version 1 (Component)
istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12

BBa_K1520509
BBa_K1520509 Version 1 (Component)
PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter

BBa_K584011
BBa_K584011 Version 1 (Component)
Lac-Lux hybrid promotor + CrtEBI + CI repressor + INP

BBa_J40000
BBa_J40000 Version 1 (Component)
Quorum sensing promoter with lac I and CFP

BBa_K1113411
BBa_K1113411 Version 1 (Component)
Targeting sequence for the delivery of the LacZ gene to the Carboxysome

BBa_K1222004
BBa_K1222004 Version 1 (Component)
pCam(T7 promoter+lac operator+CamR antisense 2+T7 terminator)

BBa_K726009
BBa_K726009 Version 1 (Component)
T7 driven lac operated inducer for the rhl quorum-sensing system

BBa_J22121
BBa_J22121 Version 1 (Component)
Lac Y gene under the rec A(SOS) promoter in plasmid pSB2K3

iGEM Parts Registry
igem_collection Version 1 (Collection)
The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.0 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are SynBioHub integration, local repositories, importing of parts/sequences from existing files, import and export of GenBank and FASTA files, extended role ontology support, the ability to partially open designs with multiple root ComponentDefinitions, backward compatibility with SBOL 1.1, and versioning.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.