BBa_K391001
BBa_K391001 Version 1 (Component)
Construct for characterization of P2 activity in the presence of AIP.

BBa_K391000
BBa_K391000 Version 1 (Component)
Construct for characterization of P2 activity in the presence of AIP.

BBa_I8035
BBa_I8035 Version 1 (Component)
Booperator aiia test 6: Effect of tetR on production of LuxR

BBa_I8033
BBa_I8033 Version 1 (Component)
Booperator aiia test 4: effect of tetR on production of RhlR

BBa_K1707028
BBa_K1707028 Version 1 (Component)
CFP-ssRA downstream of a cI regulated promoter

BBa_I744002
BBa_I744002 Version 1 (Component)
feruloyl-CoA hydratase for biosynthesis of vanillin

BBa_I8032
BBa_I8032 Version 1 (Component)
Booperator aiia test 3: effect of tetR on production of RhlR

BBa_I8034
BBa_I8034 Version 1 (Component)
Booperator aiia test 5: effect of tetR on production of LuxR

BBa_K731700
BBa_K731700 Version 1 (Component)
Platform for terminators analysis under the control of T7 promoter

BBa_I5410
BBa_I5410 Version 1 (Component)
Olmecs: Oscillator 2 of Synchro-Dualator

BBa_K195408
BBa_K195408 Version 1 (Component)
This part is composed of PelB,F10scFv and C-IgAP

BBa_K1420000
BBa_K1420000 Version 1 (Component)
Mer operon, biological system found to detoxify organic and inorganic forms of mercury

BBa_M36028
BBa_M36028 Version 1 (Component)
phiX174 gene D - mutated rbs and start codon of E, and rbs of J

BBa_K1180003
BBa_K1180003 Version 1 (Component)
Fusion protein of lamp 2b and pre-s1 for liver targeting of the exosome

BBa_K1332004
BBa_K1332004 Version 1 (Component)
The 5?? side of the intron(+exon fragment) from td gene of T4 phage

BBa_K1159304
BBa_K1159304 Version 1 (Component)
Signal Peptide of Ig Kappa chain from <i>Mus musculus</i> in RFC[25] N-part

BBa_J70031
BBa_J70031 Version 1 (Component)
Linker for BioScaffold Parts, example of Gamma BioScaffold part

BBa_J70634
BBa_J70634 Version 1 (Component)
Upstream genomic DNA fragment of Mesoplasma florum for recombination experiment

BBa_I739018
BBa_I739018 Version 1 (Component)
Learning System (Composite of BBa_I739006 and BBa_I739007)

BBa_K415109
BBa_K415109 Version 1 (Component)
PLuxR/cI-OR : RBS : pVIII

BBa_I2043
BBa_I2043 Version 1 (Component)
AHL regulated synthesis of LacI

BBa_K1523102
BBa_K1523102 Version 1 (Component)
The translational unit of yieF

BBa_J09280
BBa_J09280 Version 1 (Component)
motB under control of pLacI

HisTag-GFP
BBa_K1602023 Version 1 (Component)
Inducible generator of HisTag-GFP

BBa_I0040
BBa_I0040 Version 1 (Component)
tetR-regulatable expression of lacZalpha

BBa_K1935011
BBa_K1935011 Version 1 (Component)
DSIP under control of pU6

BBa_I739012
BBa_I739012 Version 1 (Component)
Composite of BBa_I739002 and BBa_I739003

BBa_I739020
BBa_I739020 Version 1 (Component)
Composite of BBa_I739018 and BBa_I739019

BBa_K415110
BBa_K415110 Version 1 (Component)
PLuxR/cI-OR : RBS : pIII

BBa_I739017
BBa_I739017 Version 1 (Component)
Composite of BBa_I739015 and BBa_I739016

BBa_K077044
BBa_K077044 Version 1 (Component)
AiiA undercontrol of cI and cI under control of HSL induction

BBa_K1982003
BBa_K1982003 Version 1 (Component)
CIBN(the N-terminal fragment of CIB1)

pSC101 ts
BBa_I50052 Version 1 (Component)
pSC101 temperature sensitive origin of replication

BBa_K783031
BBa_K783031 Version 1 (Component)
This is a MoClo converted version of BBa_J23114

BBa_K783032
BBa_K783032 Version 1 (Component)
This is a MoClo converted version of BBa_J23115

BBa_K783028
BBa_K783028 Version 1 (Component)
This is a MoClo converted version of BBa_J23110

BBa_K783024
BBa_K783024 Version 1 (Component)
This is a MoClo converted version of BBa_R0063

BBa_K2041017
BBa_K2041017 Version 1 (Component)
the binding site of miR-21

BBa_M31902
BBa_M31902 Version 1 (Component)
HpaI to BamHI section of M13K07 genome

BBa_K175001
BBa_K175001 Version 1 (Component)
trbK (entry exclusion) of IncP beta R751 plasmid

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_K1935007
BBa_K1935007 Version 1 (Component)
pFRQ - regulatory sequence of the circadian clock in Neurospora crassa

BBa_K395603
BBa_K395603 Version 1 (Component)
alcohol acetyltransferase; converts butanol or 2-methylbutanol to butyl acetate or 2-methylbutyl ace

BBa_K1390019
BBa_K1390019 Version 1 (Component)
Complete sMMO of Methylococcus capsulatus

BBa_K880002
BBa_K880002 Version 1 (Component)
GFP fluorescence reporter to assay the activity of FimE K137007 and HbiF K880000 recombinases.

BBa_K1945004
BBa_K1945004 Version 1 (Component)
N-terminal fragment of split T7 RNA polymerase

BBa_K1758333
BBa_K1758333 Version 1 (Component)
Lead repressor under control of constitutive promoter and strong RBS and lead responsive promoter wi

BBa_J70524
BBa_J70524 Version 1 (Component)
hard codes sequence of J70514 into a part

BBa_J70523
BBa_J70523 Version 1 (Component)
hard codes sequence of J70518 into a part

BBa_J70521
BBa_J70521 Version 1 (Component)
hard codes sequence of J70516 into a part