BBa_K2044010
BBa_K2044010 Version 1 (Component)
Based on our project, <5,7> is the direct pathway from Site No.5to Site No.7 in the map we design.

BBa_M34567
BBa_M34567 Version 1 (Component)
converts PoPS to foobob

Adapter Bi
BBa_K1807000 Version 1 (Component)
Protein generator device suitable for blue-white screening and Gibson Assembly.

BBa_K187023
BBa_K187023 Version 1 (Component)
GFP in pBA, BioBytes plasmid

BBa_K187019
BBa_K187019 Version 1 (Component)
RFP in pAB, Biobytes plasmid

BBa_K187026
BBa_K187026 Version 1 (Component)
terminator in pBA, Biobytes plasmid

BBa_K112306
BBa_K112306 Version 1 (Component)
{lambda holin!} in BBb format

BBa_K137033
BBa_K137033 Version 1 (Component)
Device with GFP with (AC)21 repeat after start codon

BBa_K112617
BBa_K112617 Version 1 (Component)
< HA!.b1006 in BBb

pCMV-ECFP-
BBa_I763023 Version 1 (Component)
LacI coding device with ECFP as a reporter regulated by pCMV

BBa_K1321317
BBa_K1321317 Version 1 (Component)
Anderson J23112-RFP in pSEVA331-Bb

BBa_K187015
BBa_K187015 Version 1 (Component)
Chloramphenicol resistance in pAB, Biobytes plasmid

BBa_K187022
BBa_K187022 Version 1 (Component)
Ampicillin resistance in pBA, Biobytes plasmid

BBa_J04431
BBa_J04431 Version 1 (Component)
GFP Coding Device with promoter, RBS, GFP with LVA tag, and Terminator

BBa_K1866010
BBa_K1866010 Version 1 (Component)
Promoter+RibosomeBindingSite+NosZ+SuperYellowFluorescenceProtein (in pSB1C3)

BBa_K812233
BBa_K812233 Version 1 (Component)
sfGFP in pCS2+ with elastase promoter

BBa_K1866002
BBa_K1866002 Version 1 (Component)
Promoter+RibosomeBindingSite+NrfA+SYFP (in pSB1K3)

BBa_K1484005
BBa_K1484005 Version 1 (Component)
AmilCP chromoprotein in BBa and MoClo standard

BBa_J72078
BBa_J72078 Version 1 (Component)
{double terminator}, b0015 in BglBrick format

BBa_K187021
BBa_K187021 Version 1 (Component)
Kanamycin resistance gene in pBA, BioBytes plasmid

BBa_K2123117
BBa_K2123117 Version 1 (Component)
Novel RFP device regulated by mercury: MerR (regulatory protein) + Stationary phase with mer operato

BBa_K1616003
BBa_K1616003 Version 1 (Component)
VVD link to YC155 (YFP Cter split)

BBa_K1616004
BBa_K1616004 Version 1 (Component)
VVD linked to YN155 (YFP Nter split)

BBa_I733005
BBa_I733005 Version 1 (Component)
Produce GFP in presence of AHL

BBa_S03736
BBa_S03736 Version 1 (Component)
pLac-lox-RBS-Tet (in pSB1A2)

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

BBa_J04795
BBa_J04795 Version 1 (Component)
Riboswitch designed to turn "ON" a protein

BBa_K1510105
BBa_K1510105 Version 1 (Component)
sRNA targets histidine kinase 11 mRNA in S.mutans

SEGA
SEGA_collection Version 1 (Collection)
In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.

BBa_K1796201
BBa_K1796201 Version 1 (Component)
An unloaded sgRNA that contains BbsI cutting site, with a promoter and terminator.

BBa_K1321362
BBa_K1321362 Version 1 (Component)
sfGFP fused to CBDcex driven by LacI

BBa_K199165
BBa_K199165 Version 1 (Component)
pT7 + RBS + GFP + TT in pSB3T5

BBa_K187005
BBa_K187005 Version 1 (Component)
Sigma 70 25% promoter in pAB, BioBytes plasmid

BBa_K187007
BBa_K187007 Version 1 (Component)
Sigma 70 75% promoter in pAB, Biobytes plasmid

BBa_K187014
BBa_K187014 Version 1 (Component)
Sigma 70 100% promoter in pBA, Biobytes plasimd

BBa_K187006
BBa_K187006 Version 1 (Component)
Sigma 70 50% promoter in pAB, Biobytes plasmid

BBa_K187003
BBa_K187003 Version 1 (Component)
Sigma 70 1% promoter in pAB BioBytes plasmid

BBa_K187004
BBa_K187004 Version 1 (Component)
Sigma 70 10% promoter in pAB, Biobytes plasmid

BBa_K812133
BBa_K812133 Version 1 (Component)
sfGFP with kozak sequence for expression in Xenopus

BBa_K1947020
BBa_K1947020 Version 1 (Component)
This polypeptide will be fused with the recombinant protein which we want to purify.

BBa_K1615108
BBa_K1615108 Version 1 (Component)
mRFP fused to CBDclos driven by LacI promoter

BBa_K730002
BBa_K730002 Version 1 (Component)
The complete denitrification of nitrate by bacteria to dinitrogen (N,)

BBa_I758600
BBa_I758600 Version 1 (Component)
Screen for binding affinity of mutant cI lambda to promotor sites

BBa_K1154006
BBa_K1154006 Version 1 (Component)
Mating pheromone-induced IGPD and constitutive LDH expression in yeast

BBa_K1657006
BBa_K1657006 Version 1 (Component)
It is called GAB. It have the resistance to glyphosate and glufosinate

[OriTR]+[R
BBa_K1439002 Version 1 (Component)
This part contains a reporter gene BBa_J04450, combined with OriTR. Used to test plasmid mobility.

BBa_M11402
BBa_M11402 Version 1 (Component)
5' UTR and RBS of psbA2 gene in Synechocystis sp. PCC 6803

BBa_K2123112
BBa_K2123112 Version 1 (Component)
Tac promoter in tandem (3 repetition) with downstream mer operator + RFP (K081014)

BBa_K2020051
BBa_K2020051 Version 1 (Component)
wild type tyrosyl synthetase for use in E.coli with amber anticodon and Y32G