BBa_K861110BBa_K861110 Version 1 (Component)BcsB,regulator of cellulose synthetase
SEGASEGA_collection Version 1 (Collection)In the Standardized Genome Architecture (SEGA), genomic integration of DNA fragments is enabled by λ-Red recombineering and so-called landing pads that are a common concept in synthetic biology and typically contain features that i) enable insertion of additional genetic elements and ii) provide well-characterized functional parts such as promoters and genes, and iii) provides insulation against genome context-dependent effects. The SEGA landing pads allow for reusable homology regions and time-efficient construction of parallel genetic designs with a minimal number of reagents and handling steps. SEGA bricks, typically synthetic DNA or PCR fragments, are integrated on the genome simply by combining the two reagents (i.e. competent cells and DNA), followed by incubation steps, and successful recombinants are identified by visual inspection on agar plates. The design of the SEGA standard was heavily influenced by the Standard European Vector Architecture (SEVA). SEGA landing pads typically hosts two major genetic “control elements” that influence gene expression on the transcriptional (C1), and translational (C2) level. Furthermore, landing pads contain gadgets such as selection and counterselection markers.
BBa_K145301BBa_K145301 Version 1 (Component)lacI controlled by key/lock
BBa_I711024BBa_I711024 Version 1 (Component)Crotonyl-CoA -> butyryl-CoA ( butyryl coa dehydrogenase)
BBa_K407005BBa_K407005 Version 1 (Component)ACEYL CO REDUCTASE
Mj TyrRSBBa_K1492002 Version 1 (Component)Methanococcus jannaschii tyrosyl-tRNA synthetase
BBa_K823042BBa_K823042 Version 1 (Component)PsspK promoter regulated by sigma G
BBa_K249003BBa_K249003 Version 1 (Component)Lumazine Synthase
sigWBO_3421 Version 1 (Component) BBa_J64022BBa_J64022 Version 1 (Component)sicA RBS
BBa_K861500BBa_K861500 Version 1 (Component)FadL, Long-chain fatty acids transporter from <i>E.coli str. K12</i>
BBa_K1869002BBa_K1869002 Version 1 (Component)Inducible CFA Synthase
SigVBO_11457 Version 1 (Component) BBa_M11062BBa_M11062 Version 1 (Component)pfaC gene for omega-3 polyunsaturated fatty acid synthase
BBa_J37032BBa_J37032 Version 1 (Component)GFP expression controlled by LuxR promoter
BBa_I741018BBa_I741018 Version 1 (Component)Right facing promoter (for xylF) controlled by xylR and CRP-cAMP
BBa_K1994027BBa_K1994027 Version 1 (Component)Com-Sigma 54 fusion protein
BBa_K362004BBa_K362004 Version 1 (Component)sodA promoter is controlled by some transcription factors including SoxS.
BBa_K362005BBa_K362005 Version 1 (Component)sufA , this promoter is controlled by OxyR.
sigIBO_31604 Version 1 (Component) SigDBO_11405 Version 1 (Component) BBa_K861030BBa_K861030 Version 1 (Component)FadB, gene for fatty acid degradation from E.coli K12
sigEBO_31442 Version 1 (Component) SigOBO_11506 Version 1 (Component) SigEBO_11399 Version 1 (Component) sigOBO_28759 Version 1 (Component) BBa_I711022BBa_I711022 Version 1 (Component)Acetoacetyl-CoA -> beta-hydroxybutyryl-CoA (beta-hydroxybutyryl-CoA dehydrogenase)
BBa_K654068BBa_K654068 Version 1 (Component)Fatty ACP Reductase + Terminator
BBa_K546001BBa_K546001 Version 1 (Component)Lux pL controlled luxR with lux pR controlled AHL degrading enzyme AiiA(lva tag).
BBa_K115050BBa_K115050 Version 1 (Component)A-coA -> AA-coA
ButCoATBBa_K2052018 Version 1 (Component)Butyryl-CoA:acetate CoA-transferase
rhIIBBa_C0170 Version 1 (Component)autoinducer synthetase for N-butyryl-HSL (BHL) and HHL (no LVA)
SigZBO_10832 Version 1 (Component) SigYBO_9057 Version 1 (Component) SigGBO_11400 Version 1 (Component) SigMBO_9201 Version 1 (Component) SigBBO_11231 Version 1 (Component) sigBBO_30699 Version 1 (Component) sigHBO_2925 Version 1 (Component) SigLBO_9200 Version 1 (Component) sigZBO_30694 Version 1 (Component) SigXBO_11490 Version 1 (Component) BBa_K1507003BBa_K1507003 Version 1 (Component)Fatty Acid Desaturase 6 (FAD6)
BBa_K2057008BBa_K2057008 Version 1 (Component)Alginate lyase and microcin controlled by lasI promoter
BBa_K654069BBa_K654069 Version 1 (Component)Fatty Aldehyde Decarbonylase + Terminator
BBa_K258001BBa_K258001 Version 1 (Component)Lipase ABC transporter recognition domain (LARD 1)
BBa_K115051BBa_K115051 Version 1 (Component)AA-coA -> HMG-coA
BBa_K145300BBa_K145300 Version 1 (Component)Lactonase controlled by key/lock
sigLBO_28960 Version 1 (Component) BBa_K861042BBa_K861042 Version 1 (Component)FadA, gene for fatty acid degradation from <i>Salmonella enterica</i> LT2