YFP
BBa_K592101 Version 1 (Component)
Yellow Fluorescent Protein (YFP)

BBa_I714888
BBa_I714888 Version 1 (Component)
double direction promoter (cI/cI434)

BBa_B0023
BBa_B0023 Version 1 (Component)
TE from coliphage T7, reversed

BBa_B0060
BBa_B0060 Version 1 (Component)
Terminator (Reverse B0050)

BBa_B0063
BBa_B0063 Version 1 (Component)
Terminator (Reverse B0053)

BBa_B0061
BBa_B0061 Version 1 (Component)
Terminator (Reverse B0051)

BBa_K1442302
BBa_K1442302 Version 1 (Component)
Reverse GFP codon optimised for E.coli

T7stop
BBa_J18913 Version 1 (Component)
T7 terminator (incl. STOP)

BBa_K731722
BBa_K731722 Version 1 (Component)
T1 terminator from E. coli rrnB

RBS-rev
BBa_K1616011 Version 1 (Component)
RBS reversed

BBa_I13401
BBa_I13401 Version 1 (Component)
GFP reporter for RHS of library test constructs

BBa_K315010
BBa_K315010 Version 1 (Component)
lox2272 Reverse

BBa_B1010
BBa_B1010 Version 1 (Component)
Terninator (artificial, large, %T~<10)

BBa_K2120106
BBa_K2120106 Version 1 (Component)
reverse tetR

BBa_K1641205
BBa_K1641205 Version 1 (Component)
FRT(reverse)-pBAD(reverse)

BBa_K2120100
BBa_K2120100 Version 1 (Component)
reverse arac-pBAD

GG108
BBa_K2145103 Version 1 (Component)
GFP/RFP reporter (Reverse/Reverse) separated by three dCas clamp sites

BBa_K313013
BBa_K313013 Version 1 (Component)
loading sequence of RNA phage MS2 (reverse)

BBa_K206008
BBa_K206008 Version 1 (Component)
pBAD reverse - terminator

BBa_K081014
BBa_K081014 Version 1 (Component)
RFP protein generator - PoPS->RFP

BBa_K1051003
BBa_K1051003 Version 1 (Component)
Stop codon free GFP in RFC[23] standard

mCherry
BBa_J06702 Version 1 (Component)
mCherry, bacterial with RBS and forward terminator

BBa_J31011
BBa_J31011 Version 1 (Component)
RBS-RFP reverse

BBa_K1758377
BBa_K1758377 Version 1 (Component)
Biosensor device for detection of GHB and GBL

BBa_K093008
BBa_K093008 Version 1 (Component)
reverse BBa_R0011

scar
BBa_G0001 Version 1 (Component)
XbaI/SpeI mixed site, reverse

R5
BBa_K1442103 Version 1 (Component)
Reverse 5

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.

rev RFP
BBa_K1463520 Version 1 (Component)
E1010 RFP in reverse

BBa_K208112
BBa_K208112 Version 1 (Component)
Bi-directional terminator for Rhodobacter sphaeroides

rev promot
BBa_K1463501 Version 1 (Component)
J23100 promoter in reverse

BBa_K1087009
BBa_K1087009 Version 1 (Component)
Ptet-reverse lox-reverse PO promoter

BBa_K1641214
BBa_K1641214 Version 1 (Component)
FRT(reverse)-pBAD(reverse)-Cre

revRBS
BBa_I742130 Version 1 (Component)
Reverse RBS

BBa_K1641226
BBa_K1641226 Version 1 (Component)
loxP(reverse)

RGE
BBa_K1442102 Version 1 (Component)
Reverse GFP for E.coli

BBa_K1641210
BBa_K1641210 Version 1 (Component)
mCherry-loxP(reverse)-GFP

BBa_P1009
BBa_P1009 Version 1 (Component)
CmR (reverse)

BBa_S03726
BBa_S03726 Version 1 (Component)
RFP(reverse)-TT

BBa_K772100
BBa_K772100 Version 1 (Component)
EpCAM Detection Device

BBa_B1003
BBa_B1003 Version 1 (Component)
Terminator (artificial, small, %T~=80)

ampR
BBa_P1006 Version 1 (Component)
ampicillin resistance cassette, reverse orientation

yfp
BBa_K165005 Version 1 (Component)
Venus YFP, yeast optimized for fusion

BBa_K2120107
BBa_K2120107 Version 1 (Component)
reverse lacIq

BBa_K1768000
BBa_K1768000 Version 1 (Component)
GFP with RBS Reverse Compliment

rev RBS
BBa_K1463560 Version 1 (Component)
Reverse RBS

Plac(rev)
BBa_I742124 Version 1 (Component)
Reverse complement Lac promoter

BBa_K530019
BBa_K530019 Version 1 (Component)
HHO1 Yeast UTR

BBa_K313012
BBa_K313012 Version 1 (Component)
lox66(reverse)

BBa_B1002
BBa_B1002 Version 1 (Component)
Terminator (artificial, small, %T~=85%)