Intein_assisted_Bisection_MappingIntein_assisted_Bisection_Mapping_collection Version 1 (Collection)Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.
BBa_K1189029BBa_K1189029 Version 1 (Component)TALE-A with a his tag linked to a K coil under the control of a LacI promoter
BBa_M45102BBa_M45102 Version 1 (Component)Cobalt detection Biobrick. RFP made in presence of Cobalt. Note the receptor also works in the prese
YFP_SPferBBa_K809314 Version 1 (Component)YFP + signal peptide of FER
BBa_M31513BBa_M31513 Version 1 (Component)Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III
BBa_M31516BBa_M31516 Version 1 (Component)Refactored portion of M13 genome from unique Hpal site on gene II to the BamHI site on gene III
iGEM Parts Registryigem_collection Version 1 (Collection)The iGEM Registry is a growing collection of genetic parts that can be mixed and matched to build synthetic biology devices and systems. As part of the synthetic biology community's efforts to make biology easier to engineer, it provides a source of genetic parts to iGEM teams and academic labs.
BBa_K987000BBa_K987000 Version 1 (Component)This part is a coding part that produces Vip3Ca3, a protein that can deals with different forms of p
BBa_I13035BBa_I13035 Version 1 (Component)3OC<sub>6</sub>HSL Receiver Device with Inducible Control of LuxR and a YFP Output device
BBa_I741109BBa_I741109 Version 1 (Component)Lambda Or operator region
BBa_K189060BBa_K189060 Version 1 (Component)Gp15 of Bacteriophage Mu
BBa_M11085BBa_M11085 Version 1 (Component)E coli outer membrane protein C (ompC) with BamHI RE site for insertion of gene to be expressed on o
BBa_K1676120BBa_K1676120 Version 1 (Component)Mutant 16 of Lactate Dehydrogenase
BBa_K1676114BBa_K1676114 Version 1 (Component)Mutant 10 of Lactate Dehydrogenase
BBa_K1361007BBa_K1361007 Version 1 (Component)Curli Fiber generator under the control of Pbad promoter with CsgA modified by His tag at a relative
BBa_K2082252BBa_K2082252 Version 1 (Component)RFP under the control of an optimized lacZ promoter with lambda cI binding site combined with SH2:cI
BBa_K1228001BBa_K1228001 Version 1 (Component)A fragment of loctoferrin
BBa_K2041010BBa_K2041010 Version 1 (Component)plac-RBS-Bxb1-T-plux-RBS-luxR-T-plux-recognition site of Bxb1-RBS-luxI-RBS-GFP-T
BBa_K809108BBa_K809108 Version 1 (Component)Efficiency test of Q0255 Terminator
RFP_SPtim2BBa_K809303 Version 1 (Component)RFP + signal peptide of TIM21
BBa_K337088BBa_K337088 Version 1 (Component)Fragment 7 of wt AAV6
BBa_K337060BBa_K337060 Version 1 (Component)Fragment 3 of wt AAV1
T25 domainBBa_K1088056 Version 1 (Component)T25 domain of CyaA from Bordetella pertussis
BBa_K1795400BBa_K1795400 Version 1 (Component)Truncated pSB1C3 with no Prefix or Suffix
BBa_K1351017BBa_K1351017 Version 1 (Component)SdpI with RBS: Immunity against the cannibalsim toxin sdpC of <i>B. subtilis</i>
BBa_M31786BBa_M31786 Version 1 (Component)1st half of Gene III- preBamHI cut
BBa_K648103BBa_K648103 Version 1 (Component)RecA with mutation of Arg 243
CsgDBBa_K1019001 Version 1 (Component)CsgD: positive regulator of curlin genes
BBa_K648102BBa_K648102 Version 1 (Component)RecA with mutation of Lys 286
BBa_K228013BBa_K228013 Version 1 (Component)(pSal PO) OR Gate - GFP
BBa_K1114003BBa_K1114003 Version 1 (Component)The MoClo format of BBa_J23103 with AB fusion sites.
PrtDEFBBa_K258007 Version 1 (Component)Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi
BBa_K831011BBa_K831011 Version 1 (Component)istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12
BBa_K831012BBa_K831012 Version 1 (Component)istR (inhibitor of SOS-induced toxicity by RNA) is small ncRNA of Escherichia coli K12
BBa_I733005BBa_I733005 Version 1 (Component)Produce GFP in presence of AHL
BBa_K1725317BBa_K1725317 Version 1 (Component)Member of the RBS library - derived from BBa_B0032
BBa_I12026BBa_I12026 Version 1 (Component)Test of BBa_R0011 (LacI regulated) using YFP
BBa_J11009BBa_J11009 Version 1 (Component)variant of J11005 (low temp color) with stronger RBS
BBa_J14461BBa_J14461 Version 1 (Component)Composite part comprised of R0051 and I13507
BBa_K1947017BBa_K1947017 Version 1 (Component)We verify the expression effect of Mms13.
BBa_J14463BBa_J14463 Version 1 (Component)Composite part comprised of J13002 and I13501
BBa_J14462BBa_J14462 Version 1 (Component)Composite part comprised of J13002 and J04650
BBa_K415011BBa_K415011 Version 1 (Component)PtetR : RBS : LuxR : Term : PluxR/cI-OR : RBS : mCherry : Term : Plux/cI-OR : RBS : LuxI
BBa_K1051262BBa_K1051262 Version 1 (Component)The measurement pathway of degradation tag K1051208.
BBa_K1051260BBa_K1051260 Version 1 (Component)The measurement pathway of degradation tag K1051206.
BBa_K1051261BBa_K1051261 Version 1 (Component)The measurement pathway of degradation tag K1051207.