BBa_K323080
BBa_K323080 Version 1 (Component)
CMV ATG cYFP link Zif268 link His stop - part of mammalian split/FRET system

BBa_P2002
BBa_P2002 Version 1 (Component)
PoPS->Zif23-GCN4

BBa_K634014
BBa_K634014 Version 1 (Component)
Reversed J23100-alkS

BBa_M1016
BBa_M1016 Version 1 (Component)
PhaB with zif23-GCN4

BBa_M1019
BBa_M1019 Version 1 (Component)
Silver Fusion Compatible PhaB gene with zif23-GCN4

BBa_M1018
BBa_M1018 Version 1 (Component)
PhaB with zif23-GCN4

BBa_K818310
BBa_K818310 Version 1 (Component)
AmilGFP with alsT regulator

BBa_K1072004
BBa_K1072004 Version 1 (Component)
alpha-ALS Coding Region

BBa_K412004
BBa_K412004 Version 1 (Component)
Codes for transcription factor AlkS

BBa_M36250
BBa_M36250 Version 1 (Component)
PalkB inducible promoter activated by AlkS

BBa_M41004
BBa_M41004 Version 1 (Component)
AlkS coding sequence in Pseudomonas putida

BBa_P20020
BBa_P20020 Version 1 (Component)
Constitutive expression of Zif23-GCN4

BBa_M11082
BBa_M11082 Version 1 (Component)
ZntR:Zn(II)-responsive regulator of zntA (also MerR transcriptional regulator)

BBa_K165056
BBa_K165056 Version 1 (Component)
Zif268-HIV repressor reporter

BBa_K539652
BBa_K539652 Version 1 (Component)
promoter (lacI regulated) + a RBS (B0032)+Alss with a RBS (B0032)+ilvC

BBa_K110000
BBa_K110000 Version 1 (Component)
Gene that smells like ass

BBa_K1072005
BBa_K1072005 Version 1 (Component)
PT7+RBS+α-ALS+T7TM

BBa_M36919
BBa_M36919 Version 1 (Component)
ZntR:Zn(II)-responsive regulator of zntA (also MerR transcriptional regulator)

BBa_K1072006
BBa_K1072006 Version 1 (Component)
Lux pR+RBS+α-ALS+2TM

BBa_K1072021
BBa_K1072021 Version 1 (Component)
Lux PR+RBS+α-ALS+2TM

BBa_K539671
BBa_K539671 Version 1 (Component)
promoter (lacI regulated)+RBS+Alss with a RBS (B0030)+ilvC+a RBS (B0030)+ilvD

BBa_M1017
BBa_M1017 Version 1 (Component)
Silver Fusion Compatible PhaB gene with zif23-GCN4

BBa_K539691
BBa_K539691 Version 1 (Component)
promoter(lacI regulated)+Alss+ilvC+ilvD(each preceded by own RBS)and RNA thermometer+terminator

BBa_K165101
BBa_K165101 Version 1 (Component)
Zif268-HIV bs + LexA bs + mCYC + Zif268-HIV repressor (mCherryx2 tagged) on pRS304*

BBa_K887004
BBa_K887004 Version 1 (Component)
Plac+alsS+ilvC+ilvD(each preceded by own zinc-finger and RBS)+Ptet+B0032+kivD+B0015

BBa_K887002
BBa_K887002 Version 1 (Component)
Plac+B0034+alsS+B0034+ilvC+B0034+ilvD+37℃RBS+tetR+double terminator+Ptet+B0032+kivD+B0015

BBa_K357003
BBa_K357003 Version 1 (Component)
3-oxoadipate enol-lactonase (also known as beta-ketoadipate enol-lactone hydrolase)

BBa_M45102
BBa_M45102 Version 1 (Component)
Cobalt detection Biobrick. RFP made in presence of Cobalt. Note the receptor also works in the prese

BBa_K1072007
BBa_K1072007 Version 1 (Component)
Lux pI+RBS+LuxR+2TM+Lux pR+RBS+LuxI+2TM+Lux pR+RBS+GFP+2TM+Lux pR+RBS+α-ALS+2TM

Bacillus subtilis Collection
bsu_collection Version 1 (Collection)
This collection includes information about promoters, operators, CDSs and proteins from Bacillus subtilis. Functional interactions such as transcriptional activation and repression, protein production and various protein-protein interactions are also included.

SBOLDesigner CAD Tool
SBOLDesigner Version 3.1 (Agent)
SBOLDesigner is a simple, biologist-friendly CAD software tool for creating and manipulating the sequences of genetic constructs using the Synthetic Biology Open Language (SBOL) 2 data model. Throughout the design process, SBOL Visual symbols, a system of schematic glyphs, provide standardized visualizations of individual parts. SBOLDesigner completes a workflow for users of genetic design automation tools. It combines a simple user interface with the power of the SBOL standard and serves as a launchpad for more detailed designs involving simulations and experiments. Some new features in SBOLDesigner are the ability to add variant collections to combinatorial derivations, enumerating those collections, and the ability to view sequence features hierarchically. There are also some small changes to the way that preferences work in regards to saving a design with incomplete sequences.

Intein_assisted_Bisection_Mapping
Intein_assisted_Bisection_Mapping_collection Version 1 (Collection)
Split inteins are powerful tools for seamless ligation of synthetic split proteins. Yet, their use remains limited because the already intricate split site identification problem is often complicated by the requirement of extein junction sequences. To address this, we augmented a mini-Mu transposon-based screening approach and devised the intein-assisted bisection mapping (IBM) method. IBM robustly revealed clusters of split sites on five proteins, converting them into AND or NAND logic gates. We further showed that the use of inteins expands functional sequence space for splitting a protein. We also demonstrated the utility of our approach over rational inference of split sites from secondary structure alignment of homologous proteins. Furthermore, the intein inserted at an identified site could be engineered by the transposon again to become partially chemically inducible, and to some extent enabled post-translational tuning on host protein function. Our work offers a generalizable and systematic route towards creating split protein-intein fusions and conditional inteins for protein activity control.