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BBa_I715013

BBa_I715013 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_I715013

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Will DeLoache

Date created: 2007-07-04 11:00:00

Date modified: 2015-08-31 04:07:49

pLac-lox-RFP(reverse)-TT-lox-RBS-Tet (psB3K3)

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Details

Types	DnaRegion
Roles	engineered_region Reporter
Sequences	BBa_I715013_sequence (Version 1)

Description

This part is designed to report the presence of functional Cre Recombinase. We plan to put it into plasmid pSB3K3 and cotransform it with BBa_I715012 to test the functionality of our Cre Recombinase with a hixC site inserted between amino acids 190 and 191.

Notes

This part is in plasmid pSB1A2. We plan to put it into pSB3K3 to perform a cotransformation with BBa_I715012.

Source

See Subparts.

Components

Access	Instance	Definition
public public public public public public public public	BBa_R0010 BBa_J61046 BBa_J31008 BBa_B0010 BBa_B0012 BBa_J61046 BBa_B0034 BBa_J31007	LacI lox mRFPr BBa_B0010 BBa_B0012 lox BBa_B0034 tetA(C)f

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010 BBa_J61046 BBa_J31008 BBa_B0010 BBa_B0012 BBa_J61046 BBa_B0034 BBa_J31007	1,200 209,242 251,928 937,1016 1025,1065 1074,1107 1116,1127 1134,2324	LacI lox mRFPr BBa_B0010 BBa_B0012 lox BBa_B0034 tetA(C)f

Other Properties

igem#experience	Fails
igem#sampleStatus	Not in stock
igem#status	Unavailable
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_I715013/1