Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Malcolm Campbell
Date created: 2009-05-28 11:00:00
Date modified: 2015-08-31 04:07:50
Burnt Pancake Tester with RE and backwards Tet resistance
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_I715065_sequence (Version 1) |
Description
This has backwards tet resistance (Tet_rev+RBS_rev) for testing backwards transcription from the promoter pLac. It also has a Hin-flippable promoter pLac.Notes
With the RE, recombination enhancer, the flipping goes faster. This is not the construct we used in our Burnt Pancake paper.Source
It comes from subparts in the registry.| Access | Instance | Definition |
| public public public public public public public public | BBa_J31006 BBa_J44001 BBa_J44000 BBa_R0010 BBa_J44000 BBa_B0034 BBa_E0040 BBa_J3101 | tetA(C)b BBa_J44001 hixC LacI hixC BBa_B0034 GFP RE |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J31006 BBa_J44001 BBa_J44000 BBa_R0010 BBa_J44000 BBa_B0034 BBa_E0040 BBa_J3101 | 1,1191 1200,1214 1223,1248 1257,1456 1465,1490 1499,1510 1517,2236 2245,2321 | tetA(C)b BBa_J44001 hixC LacI hixC BBa_B0034 GFP RE |