Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: sarah hollingshead
Date created: 2007-10-21 11:00:00
Date modified: 2015-08-31 04:08:03
Vanillin Biosynthesis genes
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_I742140_sequence (Version 1) |
Description
Construct contains all five genes required to synthesise vanillin (the major component of vanilla scent) from tyrosine.The five genes in this pathway are, Sam5, Sam8, COMT, ech and fcs.
Details of the vanillin biosynthesis pathway may be found on the Edinburgh Yoghurt Wiki
Notes
COMT, ech and fcs were all synthesised by geneart.The genomic COMT clone contained introns, so we decided to have the cDNA sequence synthesised by geneart
Ech and Fcs genes both contain a very high GC content, which presented many difficulties during cloning. Therefore both genes were synthesised by geneart so they both had optimum codon usage for E. coli
Source
Both Sam genes (Sam5 and Sam8) were cloned from the bacterium from Saccharothrix espanaensisCOMT cDNA sequence is from Medicago sativa (the alfalfa plant), but the actual construct was synthesised by gene art.
Ech and Fcs genes both come from the bacterium Pseudomonas fluorescens.
References for these genes:
Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix esanaensis, Berner M, et al, Journal of Bacteriology 188(7): 2666, (2006)
Gowri G, et al, Molecular cloning and expression of alfalfa S-adenosyl-L-methionine: caffeic acid 3-0-methyltransferase, a key enzyme of lignin biosynthesis, Plant Physiology 97(1):7 (1991)
Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin, Guo D, et al, Plant Cell. January; 13(1): 73 (2001)
Cloning and Characterization of the Ferulic Acid Catabolic Genes of Sphingomonas paucimobilis SYK-6, E Masai, et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(9): 4416???4424 (2002)
Vanillin production using metabolically engineered Escherichia coli under non-growing conditions, P Barghini, et al, Microbial Cell Factories, 6:13 (2007)
Characterization of a bacterial tyrosine ammonia lyase, a biosynthetic enzyme for the photoactive yellow Protein, JA Kyndt et al, FEBS Letters 512: 240-244 (2002)
Vanilla flavour: production by conventional and biotechnological routes, S Ramachandra Rao & GA Ravishankar, Journal of the Science of Food and Agriculture, 80:289-304 (2000)
Two O-Methyltransferases Isolated from Flower Petals of Rosa chinensis var. spontanea Involved in Scent Biosynthesis W Shuiqin, et al, Journal of Bioscience and Bioengineering, 96(2): 119-128 (2003)
Applied biocatalysis for the synthesis of natural flavour compounds ??? current industrial processes and future prospects, J Schrader, Biotechnology Letters, 26: 463???472 (2004)
| Access | Instance | Definition |
| public public public public public public public public | BBa_I742105 BBa_I742106 BBa_J15001 BBa_I742107 BBa_J15001 BBa_I742114 BBa_J15001 BBa_I742112 | BBa_I742105 BBa_I742106 BBa_J15001 COMT BBa_J15001 BBa_I742114 BBa_J15001 BBa_I742112 |