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BBa_J47900

BBa_J47900 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J47900

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Luc Weiss

Date created: 2007-06-07 11:00:00

Date modified: 2015-08-31 04:08:51

PSU iGEM Turn on motility and reporter with autoinducer

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Details

Types	DnaRegion
Roles	Device engineered_region
Sequences	BBa_J47900_sequence (Version 1)

Description

Receiver Device
BBa_I14032 LacIQ constit Promoter
BBa_B0034 RBS
BBa_C0062 LuxR (to bind to HSL)
BBa_B0010 Terminator
BBa_B0012 Terminator

Motility/Reporter
PluxR BBa_R0062 HSL+LuxR activates
BBa_B0033 Weak RBS
BBa_J09271 motB without RBS
BBa_J52008 Rluc Luciferase
BBa_B0015 Double Terminator

Notes

motB appears to be toxic if expressed in high levels

Source

motile bacterial strains

Components

Access	Instance	Definition
public public public public public public public public public public	BBa_I14032 BBa_B0034 BBa_C0062 BBa_B0010 BBa_B0012 BBa_R0062 BBa_B0033 BBa_J09271 BBa_J52008 BBa_B0015	P(Lac) IQ BBa_B0034 luxr BBa_B0010 BBa_B0012 lux pR BBa_B0033 BBa_J09271 BBa_J52008 BBa_B0015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I14032 BBa_B0034 BBa_C0062 BBa_B0010 BBa_B0012 BBa_R0062 BBa_B0033 BBa_J09271 BBa_J52008 BBa_B0015	1,37 46,57 64,819 853,932 941,981 990,1044 1053,1063 1070,1996 2003,2938 2947,3075	P(Lac) IQ BBa_B0034 luxr BBa_B0010 BBa_B0012 lux pR BBa_B0033 BBa_J09271 BBa_J52008 BBa_B0015