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BBa_J64974

BBa_J64974 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_J64974

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: reid williams

Date created: 2007-03-20 12:00:00

Date modified: 2015-05-08 01:08:17

small molecule controlled, riboregulator based XOR gate

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_J64974_sequence (Version 1)

Description

This device uses the small molecules lac and tet as inputs and expresses GFP as an XOR function of the two.

Notes

The presence of either (but not both) of the keys unlocks a blocked RBS to allow translation of a CFP degradation tag fusion. The two keys have a greater affinity to each other than to the lock, so the presence of both does not unlock the blocked RBS.

Source

The Collins lab riboregulators and existing registry parts.

Components

Access	Instance	Definition
public public public public public public public public public public public public public	BBa_I14032 BBa_J64720 BBa_B0010 BBa_B0012 BBa_R0040 BBa_J64722 BBa_B0010 BBa_B0012 BBa_J23100 BBa_J64723 BBa_E0022 BBa_B0010 BBa_B0012	P(Lac) IQ BBa_J64720 BBa_B0010 BBa_B0012 p(tetR) BBa_J64722 BBa_B0010 BBa_B0012 BBa_J23100 BBa_J64723 ECFP BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I14032 BBa_J64720 BBa_B0010 BBa_B0012 BBa_R0040 BBa_J64722 BBa_B0010 BBa_B0012 BBa_J23100 BBa_J64723 BBa_E0022 BBa_B0010 BBa_B0012	1,37 46,149 158,237 246,286 295,348 357,460 469,548 557,597 606,640 649,700 707,1468 1477,1556 1565,1605	P(Lac) IQ BBa_J64720 BBa_B0010 BBa_B0012 p(tetR) BBa_J64722 BBa_B0010 BBa_B0012 BBa_J23100 BBa_J64723 ECFP BBa_B0010 BBa_B0012