BBa_K092901

BBa_K092901 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K092901

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: EPF-Lausanne Team 2008

Date created: 2008-10-21 11:00:00

Date modified: 2015-05-08 01:08:40

RhlR, PrhlR + LacI modified , PLac + TetR, pTetR + RFP and LuxI

Details

• Types: DnaRegion
• Roles: engineered_region; Project
• Sequences: BBa_K092901_sequence (Version 1)

Description

Sequence containing :
- RhlR controlled by a constitutive promoter
- LacI modified with a RhlR promoter
- TetR controlled by a Lac Promoter
- RFP controlled by a TetR promoter
- LuxI is added at the end of the cds of the RFP.
As no terminator was placed after the RFP, they both depends on the TetR promoter. The transcription of RFP and LuxI is inhibited when TetR is present. The production of TetR depends on the promoter you put in front of it.

Source

Source of LacIm : Basu et al."A synthetic multicelluar system for programmed pattern formation", Nature. 2005 Apr 28;434(7037):1130-4

Components

Access	Instance	Definition
public	BBa_K092900	BBa_K092900
public	BBa_B0015	BBa_B0015
public	BBa_R0010	LacI
public	BBa_B0015	BBa_B0015
public	BBa_K092700	BBa_K092700

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K092900	1,2102	BBa_K092900
BBa_B0015	2111,2239	BBa_B0015
BBa_R0010	2248,2447	LacI
BBa_B0015	3167,3295	BBa_B0015
BBa_K092700	2456,4869	BBa_K092700

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K092901/1

Member of these Collections

• iGEM Parts Registry