Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lin Ang Chieh
Date created: 2013-09-14 11:00:00
Date modified: 2015-05-08 01:09:30
pCI-mCherry-pConst-RhlR-pRhl-RhlR-CI
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1157013_sequence (Version 1) |
Description
This biosensor is constructed to sense C-4 AHL molecules through a method different from BBa_K1157012. This time when AHL molecules are sensed, it produces Rhl receptors which initiates production of CI. Therefore, CI inhibits pCI and the expression of reporter mCherry. When compared to control group, the results will be expected to express lower fluorescence level when there is existence of AHL molecules.Notes
The parts are built by the original biobricks provided by iGEM official, and there are no restriction enzyme sites in the inner part of the construct.Source
This is built by the biobricks provided by iGEM official| Access | Instance | Definition |
| public public public public public public public public public public public public public public | BBa_R0051 BBa_B0030 BBa_J06504 BBa_B0015 BBa_J23100 BBa_B0030 BBa_C0171 BBa_B0015 BBa_R0071 BBa_B0030 BBa_C0171 BBa_B0030 BBa_C0051 BBa_B0015 | cI lam BBa_B0030 mCherry BBa_B0015 BBa_J23100 BBa_B0030 rhIR BBa_B0015 RhlR+C4 BBa_B0030 rhIR BBa_B0030 cI lam BBa_B0015 |