Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: paul james
Date created: 2013-09-19 11:00:00
Date modified: 2015-05-08 01:09:35
CcaS, CcaR, PcpcG2, cI(lambda), magenta
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1173501_sequence (Version 1) |
Description
This is a composite part which is responsive to green light and controls the production of a magenta pigment. CcaS is a trans-membrane protein which responds to green light by phosphorylating CcaR when green light is present. CcaR-P then binds to a specific promoter (BBa_K592003), which allows the transcription of the cI protein. This in turn binds to a specific promoter, which prevents the transcription of a gene coding for a magenta pigment (BBa_K592012). Overall, this means that exposure of the cells to green light will halt the synthesis of magenta pigment. Inversely, lack of exposure to green light will allow the synthesis of magenta pigment.Notes
To prevent the accidental transcription of CcaR and the magenta pigment, these genes were reverse complement-ed. This should prevent unwanted production of magneta pigment. We also had to deconstruct BBa_C0051, as we needed the cI promoter to go before the magenta pigment.Source
Synthesised by IDT (gBlocks). Based on a variety of BioBricks.| Access | Instance | Definition |
| public public public public public public public public public public public public public public | BBa_K608002 BBa_K592001 BBa_B0015 BBa_K608002 BBa_K592002 BBa_B0015 BBa_K592003 BBa_B0034 BBa_C0051 BBa_B0015 BBa_R0051 BBa_B0034 BBa_K592012 BBa_B0015 | BBa_K608002 BBa_K592001 BBa_B0015 BBa_K608002 BBa_K592002 BBa_B0015 BBa_K592003 BBa_B0034 cI lam BBa_B0015 cI lam BBa_B0034 eforRed BBa_B0015 |