Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alişan Kayab??len
Date created: 2013-10-01 11:00:00
Date modified: 2015-05-08 01:09:39
Catalytically active CYP6G1 coding device
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1197015_sequence (Version 1) |
Description
This device was constructed to produce catalytically active CYP6G1 in B. subtilis and secrete it outside. Secretion is needed since CYP6G1 enzyme should degrade imidacloprid in bee midgut. To provide constitutively production and secretion of this enzyme, we used Pveg constitutive promoter, SpoVG RBS with SacB signal peptide (K541501) which is used by Fatih_Turkey Team in 2011.Also fCPR production is necessary to make CYP6G1 enzyme catalytically active. Thus, SpoVG RBS and SacB signal peptide was used before fCPR too.
Notes
Promoter and RBS are specific for expression and signal peptide is specific for secretion in B. subtilis.Source
CYP6G1 comes from Drosophila melanogaster genome and fCPR comes from Musca domestica.| Access | Instance | Definition |
| public public public public public | BBa_K541501 BBa_K1197013 BBa_K606061 BBa_K1197016 BBa_B0015 | BBa_K541501 BBa_K1197013 BBa_K606061 BBa_K1197016 BBa_B0015 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K541501 BBa_K1197013 BBa_K606061 BBa_K1197016 BBa_B0015 | 1,219 226,1803 1812,1823 1830,3950 3959,4087 | BBa_K541501 BBa_K1197013 BBa_K606061 BBa_K1197016 BBa_B0015 |